Induction of oil palm (Elaeis guineensis Jacq. var. Tenera) callogenesis and somatic embryogenesis from young leaf explants

Constantin, Mondjeli; Nchu, Walter Ajambang; Godswill, Ntsomboh-Ntsefong; Wiendi, Ni Made Armini; Wachjar, Ade; Frank, Ngando Ebongue Georges

doi:10.7324/jabb.2015.3402

Cited by 10 publications

(8 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar response was reported by [23] to the induction of maximum callus at 89% in contribution of lower concentrations of auxin 2, 4-D and picloram on oil palm. However, our result was contradicted to [24] whom reported the application of higher concentrations of PGRs to initiate callus from young leaf explant of tenera palm.…”

Section: Discussioncontrasting

confidence: 99%

Embryogenic Callus Induction of Arenga pinnata Wurmb Merr. (Sugar palm) from Basal Stem Explant

2016

IJAAEE

View full text Add to dashboard Cite

The present study investigated the induction of embryogenic callus from basal stem explant of sugar palm (Arenga pinnata) obtained from in vitro seedlings. The primary callus induction from basal stem explant was highly dependent on genotype and concentrations of plant growth regulators (PGRs) used. Optimum primary callus induction was determined on MS [1] medium supplemented with 2, 4-Dichlorophenoxyacetic acid (2,4-D) in combination with Kinetin (Kin). Embryogenic calli were obtained on similar medium after 12 weeks incubation. The primary callus induced was in the form of translucent white floss and gradually grown as embryogenic compact callus within 4-8 weeks of culture. The optimum callus induction frequency was recorded at 70% with the optimum mean diameter of 0.850±0.17 cm and mean fresh weight of 0.450±0.06 g. Embryogenic calli were proliferated in silver nitrate (AgNO 3) added medium.

show abstract

Section: Discussioncontrasting

confidence: 99%

Embryogenic Callus Induction of Arenga pinnata Wurmb Merr. (Sugar palm) from Basal Stem Explant

2016

IJAAEE

View full text Add to dashboard Cite

show abstract

“…Indeed, EC induction is greatly influenced by concentrations of auxins with or without activated charcoal (AC) (9–10 μM without AC or 450 μM with 2.5–3 g/L AC) (Table 1). Furthermore, the embryogenic callus is usually transferred to MS (Murashige and skoog’s) medium supplemented with a decreased auxin (1-naphthaleneacetic acid (NAA)) concentration for the induction and maturation of somatic embryos [3,10,27,28] (Table 1). In addition to NAA, some protocols have described the supplementation of cytokinins in negligible amounts for somatic embryogenesis (SE) induction and as well as for maturation.…”

Section: Oil Palm Propagation Methodsmentioning

confidence: 99%

“…Micropropagation of oil palm via somatic embryogenesis has been successfully achieved from different explants, such as mature zygotic embryos [25,34,35,36,37] (Table 1), immature zygotic embryos [38,39] (Table 1), immature male inflorescence [10] (Table 1), immature female inflorescence [40,41] (Table 1), mature leaves [20] (Table 1), immature leaves [27,28,31,42,43], young plantlets [10,44,45] (Table 1), and shoots [46] (Table 1). Most of the above-mentioned reports described the propagation of oil palm through indirect somatic embryogenesis, in which the callus is the intervening phase.…”

Section: Oil Palm Propagation Methodsmentioning

confidence: 99%

Progress in Tissue Culture and Genetic Transformation of Oil Palm: An Overview

Yarra

Jin

Zhao

et al. 2019

IJMS

View full text Add to dashboard Cite

Oil palm (Elaeis guineensis, Jacq.) is a prominent vegetable-oil-yielding crop. Cultivating high-yielding oil palm with improved traits is a pre-requisite to meet the increasing demands of palm oil consumption. However, tissue culture and biotechnological approaches can resolve these concerns. Over the past three decades, significant research has been carried out to develop tissue culture and genetic transformation protocols for oil palm. Somatic embryogenesis is an efficient platform for the micropropagation of oil palm on a large scale. In addition, various genetic transformation techniques, including microprojectile bombardment, Agrobacterium tumefaciens mediated, Polyethylene glycol mediated mediated, and DNA microinjection, have been developed by optimizing various parameters for the efficient genetic transformation of oil palm. This review mainly emphasizes the methods established for in vitro propagation and genetic transformation of oil palm. Finally, we propose the application of the genome editing tool CRISPR/Cas9 to improve the various traits in this oil yielding crop.

show abstract

“…For induction and maturation of SEs, EC are transferred to a medium with reduced auxin concentration. Sometimes, the concentration of auxins is gradually reduced with each subculture step (Constantin et al, 2015; Jayanthi et al, 2015; Hashim et al, 2018). The auxins picloram and 2,4-D that are commonly used during the EC initiation phase, are often replaced by the weaker auxin 1-naphthaleneacetic acid (NAA).…”

Section: Oil Palm Somatic Embryogenesismentioning

confidence: 99%

“…Because of the sturdy architecture of the oil palm, there is very limited choice of explants that can be used for initiation of embryogenic cultures. Typically, immature leaf explants are being used for commercial production of tissue culture plantlets (Schwendiman et al, 1988; Constantin et al, 2015; Corrêa et al, 2016), but not without difficulties.…”

Section: Problems Associated With Oil Palm Somatic Embryogenesismentioning

confidence: 99%

Tissue Culture of Oil Palm: Finding the Balance Between Mass Propagation and Somaclonal Variation

Weckx¹,

Inzé

Maene³

2019

Front. Plant Sci.

View full text Add to dashboard Cite

The oil palm ( Elaeis guineensis Jacq.) is typically propagated in vitro by indirect somatic embryogenesis, a process in which somatic cells of an explant of choice are, via an intermediate phase of callus growth, induced to differentiate into somatic embryos. The architecture of the oil palm, lacking axillary shoots, does not allow for vegetative propagation. Therefore, somatic embryogenesis is the only alternative to seed propagation, which is hampered by long germination times and low germination rates, for the production of planting material. The current oil palm somatic embryogenesis procedure is associated with several difficulties, which are described in this review. The limited availability of explants, combined with low somatic embryo initiation and regeneration rates, necessitate the proliferation of embryogenic structures, increasing the risk for somaclonal variants such as the mantled phenotype. Several ways to improve the efficiency of the tissue culture method and to reduce the risk of somaclonal variation are described. These include the use of alternative explants and propagation techniques, the introduction of specific embryo maturation treatments and the detection of the mantled abnormality in an early stage. These methods have not yet been fully explored and provide interesting research field for the future. The development of an efficient oil palm micropropagation protocol is needed to keep up with the increasing demand for palm oil in a sustainable way. Mass production of selected, high-yielding palms by tissue culture could raise yields on existing plantations, reducing the need for further expansion of the cultivated area, which is often associated with negative environmental impacts.

show abstract

Induction of oil palm (Elaeis guineensis Jacq. var. Tenera) callogenesis and somatic embryogenesis from young leaf explants

Cited by 10 publications

References 1 publication

Embryogenic Callus Induction of Arenga pinnata Wurmb Merr. (Sugar palm) from Basal Stem Explant

Embryogenic Callus Induction of Arenga pinnata Wurmb Merr. (Sugar palm) from Basal Stem Explant

Progress in Tissue Culture and Genetic Transformation of Oil Palm: An Overview

Tissue Culture of Oil Palm: Finding the Balance Between Mass Propagation and Somaclonal Variation

Contact Info

Product

Resources

About