Induction of ovarian follicular wave emergence and ovulation in progestin-based timed artificial insemination protocols for Bos indicus cattle

Filho, Manoel Francisco de Sá; Baldrighi, J.M.; Sales, J. N. S.; Crepaldi, Gabriel Armond; Carvalho, João Batista Pereira de; Bó, G. A.; Baruselli, Pietro Sampaio

doi:10.1016/j.anireprosci.2011.12.005

Cited by 52 publications

(28 citation statements)

References 42 publications

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“…In this way, the association of estrus detection and FTAI protocols could be beneficial to improve pregnancy rates, as observed in our study. A greater number of heifers displaying estrus in the conventional (61.4%, 62/101) compared to J-Synch protocol (38.6%, 39/101, P= 0.003) was observed, in agreement with previous studies (Perry and Perry, 2008;Sa Filho et al, 2011a;Pereira et al, 2013). Administration of ECP can increase pre-ovulatory estradiol concentration, increasing the number of animals in estrus (Perry and Perry, 2008).…”

Section: Resultssupporting

confidence: 89%

“…Estrous cycle synchronization programs have been used worldwide to increase reproductive efficiency of beef cattle (Sa Filho et al, 2010;Pereira et al, 2014). Fixed-time artificial insemination (FTAI) allows an increase in the number of cows inseminated without estrous detection (Sa Filho et al, 2011a), facilitating the use of artificial insemination (AI) (Cavalieri et al, 2004) and minimizing time and labor costs (Dadarwal et al, 2013). The success of FTAI programs depends on the manipulation of several physiological processes, such as the recruitment of a new follicular wave, follicular dominance (Cerri et al, 2009) and moment of ovulation (Dadarwal et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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J-Synch protocol associated with estrus detection in beef heifers and non-lactating cows

Mion

Bonotto²,

Farias

et al. 2019

Med. Vet. (UFRPE)

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The aim of this study was to compare the J-Synch and conventional protocols associated with estrus detection in beef heifers and to compare pregnancy rate between non-lactating cows displaying estrus or not during the J-Synch protocol. In Experiment 1, heifers were subjected to timed artificial insemination (AI) in a conventional protocol with ECP (n=147) or J-Synch protocol plus eCG (n=149). The AI occurred 12 hours after estrus expression; or 48 (Conventional protocol) and 72 hours (J-Synch protocol) after device removal for animals not displaying estrus. The J-Synch group received 10 µg of GnRH at AI. In Experiment 2, the J-Synch was performed (n=116 cows), but without eCG injection, and estrus was monitored. Pregnancy rate was not different between protocols in Experiment 1 (Conventional: 50.68%; J-Synch: 60.4%). Heifers that displayed estrus had higher pregnancy rate only in the conventional protocol. In Experiment 2, pregnancy rate was not different between cows that displayed estrus or not. Therefore, performing AI earlier according to estrus expression increases pregnancy rate in conventional protocol, however it does not increase pregnancy rate in the J-Synch protocol.Keywords: early artificial insemination; cattle; estrus behavior; GnRH-based protocol. ResumoOs objetivos desse estudo foram comparar o protocolo J-Synch e o protocolo convencional associados com detecção de estro em novilhas de corte e comparar a taxa de prenhez entre vacas não lactantes que demonstraram ou não estro após o protocolo J-Synch. No experimento 1, as novilhas foram submetidas à inseminação artificial (IA) em tempo fixo em um protocolo convencional com ECP (n=147) ou através do protocolo J-Synch com eCG (n=149). A IA ocorreu 24 horas após o estro; ou 48 (Convencional) e 72 horas (J-Synch) após a remoção do dispositivo naqueles animais que não demonstraram estro. O grupo J-Synch recebeu 10 µg de GnRH no momento da IA. No experimento 2, foi aplicado o protocolo J-Synch (n=116 vacas) sem administração de eCG e o estro foi monitorado. No Experimento 1, a taxa de prenhez não foi diferente entre os protocolos (convencional: 50,68%; J-Synch: 60,4%). Novilhas que demonstraram estro tiveram maior taxa de prenhez apenas no protocolo convencional. No Experimento 2, a taxa de prenhez não foi diferente entre as vacas que demonstraram ou não estro. Assim, antecipar o momento da IA de acordo com o estro aumenta a taxa de prenhez no protocolo convencional, contudo não aumenta a taxa de prenhez no protocolo J-Synch.Palavras-chave: antecipação da inseminação artificial; bovinos; comportamento estral; protocolos baseados em GnRH.

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Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

J-Synch protocol associated with estrus detection in beef heifers and non-lactating cows

Mion

Bonotto²,

Farias

et al. 2019

Med. Vet. (UFRPE)

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“…Although E2/P4-based FTAI protocols are widely used in B. taurus and B. indicus (Martinez et al, 2000;Ayres et al, 2008;Meneghetti et al, 2009;Souza et al, 2009;Sá Filho et al, 2011;Pereira et al, 2013b;Monteiro Jr et al, 2015b), the metabolism of E2 is different between B. taurus and B. indicus . For example, in a 3 x 2 Latin Square design study (Bastos et al, 2011), the effect of dose of estradiol benzoate (EB; 1, 2 or 4 mg), given at the same time as a P4 intravaginal insert, on the synchronization of follicular wave emergence was evaluated in non-lactating Nelore (n = 13) and Holstein (n = 11) cows receiving a maintenance diet.…”

Section: Effectiveness Of E2/p4-based Protocols For Follicle Wave Emementioning

confidence: 99%

Endocrine and metabolic differences between Bos taurus and Bos indicus cows andimplications for reproductive management

Sartori

Monteiro

Wiltbank³

2016

Anim Reprod

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Based on the considerable differences in ovarian morphology and function, as well as circulating hormones and metabolites between Bos indicus (B. indicus) and Bos taurus (B. taurus), researchers are using this acquired knowledge to optimize protocols for fixed-time artificial insemination (FTAI), and production of in vivo derived embryos by multiple ovulation or by in vitro embryo production (IVP). In B. indicus, at the time of follicle deviation, the dominant follicle is smaller and acquires ovulatory capacity at a smaller diameter than B. taurus. Moreover, despite ovulating smaller follicles and having smaller corpora lutea (CL), circulating concentrations of estradiol (E2) and progesterone (P4) are greater in B. indicus than B. taurus. These physiological differences may be related to greater circulating cholesterol, insulin and IGF1 in B. indicus than in B. taurus. For both genetic groups there is a negative relationship between circulating P4 and ovulatory response to the first GnRH treatment of a fixed-time AI (FTAI) protocol. Moreover, despite lower clearance rates of steroid hormones in B. indicus than B. taurus, the dose of 2 mg estradiol benzoate seems to be the most effective either for Nelore (B. indicus beef), Angus (B. taurus beef), or Holstein (B. taurus dairy) cows at the initiation of an E2/P4-based FTAI protocol to optimize synchronization and pregnancy per AI (P/AI). Several studies have shown that only one recommended dose of PGF2α at a FTAI protocol may be insufficient for adequate luteolysis in B. indicus and B. taurus. When submitted to multiple ovulation and embryo transfer, B. indicus cows and heifers need less FSH than B. taurus to achieve superovulation. Moreover, IVP has been more successful in B. indicus than B. taurus due to greater antral follicle count and anti-mullerian hormone, and better oocyte quality.

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“…Moreover, T1 group showing a larger ovulatory follicle had shorter interval after the second GnRH compared with the control group, which was consistent with the results in cows (Sá Filho et al., ). As many studies reported, the increased diameter of ovulatory follicle could enhance ovulation response and improve fertility caused by post‐ovulation formed larger corpora lutea that secrete more progesterone (P4) (Mussard et al., ; Sá Filho, Baldrighi et al., ). On the contrary, ovulated small and premature follicles in cows might reduce pregnancy rates and increase embryonic mortality (Santos, Thatcher, Chebel, Cerri, & Galvao, ; Vasconcelos, Sartori, Oliveira, Guenther, & Wiltbank, ).…”

Section: Discussionmentioning

confidence: 99%

Nasal immunization with inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis for improving ovarian responses and fertility in cross‐bred buffaloes

Liu

Rehman

et al. 2016

Reprod Domestic Animals

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This study was conducted to determine the effect of immunization with inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis on ovarian responses and fertility in cross-bred buffaloes. A total of 134 cross-bred buffaloes were divided into four groups: groups T1 (n = 34), T2 (n = 35) and T3 (n = 31) were nasal immunized twice a day with 10 ml of 1 × 10 CFU/ml of the C501 (pVAX-asd-IS) vaccine for 5, 3 and 1 day, respectively. Group C (n = 34) was nasal immunized with 10 ml PBS for 5 days. All animals were immunized twice with an interval of 14 days and administered with 200 μg of a GnRH analogue on day 28, 0.5 mg PGF on day 35 and 200 μg of the same GnRH analogue on day 37. TAI was performed at 18 and 24 hr after the second GnRH treatment. Fourteen days after primary immunization, C501 (pVAX-asd-IS) elicited significant immune responses, and anti-inhibin IgG antibody titres in group T1 were significantly higher (p < .01) than groups T3 and C. After the second GnRH treatment, the growth speed of the dominant follicles in group T1 was significantly faster (p < .05) than groups T3 and C. The number and diameter of large follicles (≥10 mm) as well as ovulatory follicles in group T1 were the greatest in all groups, resulting in a greater conception rate in buffaloes with positive anti-inhibin antibodies. These results demonstrate that immunization with the C501 (pVAX-asd-IS) vaccine, coupled with the Ovsynch protocol, could be used as an alternative approach to improve reproductive performance in cross-bred buffaloes.

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Induction of ovarian follicular wave emergence and ovulation in progestin-based timed artificial insemination protocols for Bos indicus cattle

Cited by 52 publications

References 42 publications

J-Synch protocol associated with estrus detection in beef heifers and non-lactating cows

J-Synch protocol associated with estrus detection in beef heifers and non-lactating cows

Endocrine and metabolic differences between Bos taurus and Bos indicus cows andimplications for reproductive management

Nasal immunization with inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis for improving ovarian responses and fertility in cross‐bred buffaloes

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