Induction of PAH-catabolism in mushroom compost and its use in the biodegradation of soil-associated phenanthrene

Reid, Brian J.; Fermor, Terry R; Semple, Kirk T.

doi:10.1016/s0269-7491(01)00239-1

Cited by 60 publications

(29 citation statements)

References 22 publications

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“…Aromatic degradation by the adapted microflora was lower than the degradation of the aliphatic fraction. These results confirm the relative recalcitrant degradation of AH fraction as described by several authors (Harayama et al 1999;Reid et al 2002). However Ozaki and Fujita (2006) described bacterial consortia which are able to degrade AH at higher rates.…”

Section: Efficiency Of Zco Degradation By Seawater Adapted Microflorasupporting

confidence: 89%

Molecular diversity analysis and bacterial population dynamics of an adapted seawater microbiota during the degradation of Tunisian zarzatine oil

et al. 2008

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The indigenous microbiota of polluted coastal seawater in Tunisia was enriched by increasing the concentration of zarzatine crude oil. The resulting adapted microbiota was incubated with zarzatine crude oil as the only carbon and energy source. Crude oil biodegradation capacity and bacterial population dynamics of the microbiota were evaluated every week for 28 days (day 7, day 14, day 21, and day 28). Results show that the percentage of petroleum degradation was 23.9, 32.1, 65.3, and 77.8%, respectively. At day 28, non-aromatic and aromatic hydrocarbon degradation rates reached 92.6 and 68.7%, respectively. Bacterial composition of the adapted microflora was analysed by 16S rRNA gene cloning and sequencing, using total genomic DNA extracted from the adapted microflora at days 0, 7, 14, 21, and 28. Five clone libraries were constructed and a total of 430 sequences were generated and grouped into OTUs using the ARB software package. Phylogenetic analysis of the adapted microbiota shows the presence of four phylogenetic groups: Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. Diversity indices show a clear decrease in bacterial diversity of the adapted microflora according to the incubation time. The Proteobacteria are the most predominant (>80%) at day 7, day 14 and day 21 but not at day 28 for which the microbiota was reduced to only one OTU affiliated with the genus Kocuria of the Actinobacteria. This study shows that the degradation of zarzatine crude oil components depends on the activity of a specialized and dynamic seawater consortium composed of different phylogenetic taxa depending on the substrate complexity.

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Section: Efficiency Of Zco Degradation By Seawater Adapted Microflorasupporting

confidence: 89%

Molecular diversity analysis and bacterial population dynamics of an adapted seawater microbiota during the degradation of Tunisian zarzatine oil

et al. 2008

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“…Therefore, improving techniques with compost application for most efficient PAH bioremediation is still a field of research, since the mechanisms underlying the observed effect of compost addition, e.g., the introduction of microorganisms, nutrients, primary substrates, or all of them, are not yet clear. Supplementing contaminated soils with various types of compost material or other organic supplements, e.g., yard waste compost (Carlstrom and Tuovinen 2003;Kästner and Mahro 1996;Kästner et al 1995;Winquist et al 2014;Wu et al 2013), spent mushroom substrate (Cajthaml et al 2002;Lau et al 2003;Reid et al 2002;Šašek et al 2003), animal manure (Atagana 2004;Wong et al 2002;Yuan et al 2009), and sludges (Hamdi et al 2006(Hamdi et al , 2007Petersen et al 2003), has been successfully applied in order to remove PAH; a detailed summary is given elsewhere (Loick et al 2009). For optimal PAH removal, fully rotted compost has often been found to be much more effective than fresh compost (Haderlein et al 2006;Plaza et al 2009), and mature compost is considered to provide available nutrients with low sorption potential for high-molecular-weight PAH (AntizarLadislao et al 2005;Hafidi et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The application of stable or radioisotopes has provided valuable process and source information in environmental systems (Gutierrez-Zamora and Manefield 2010). Research studies have been successfully conducted using 14 C-labeled PAH (Carlstrom and Tuovinen 2003;Cheng and Wong 2008;Haderlein et al 2001Haderlein et al , 2006Johnsen et al 2002;Kästner et al 1995Kästner et al , 1999Reid et al 2002) for investigating the fate of the PAH-derived carbon in pure soil, soil-compost mixtures, or composting systems. These studies, however, mainly focused on PAH mineralization.…”

Section: Introductionmentioning

confidence: 99%

Degradation of 13C-labeled pyrene in soil-compost mixtures and fertilized soil

Adam

Miltner

Kästner

2015

Appl Microbiol Biotechnol

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Polycyclic aromatic hydrocarbons (PAH) are toxic pollutants widely distributed in the environment due to natural and anthropogenic processes. In order to mitigate tar oil contaminations with PAH, research on improving bioremediation approaches, which are sometimes inefficient, is needed. However, the knowledge on the fate of PAH-derived carbon and the microbial degraders in particular in compost-supplemented soils is still limited. Here we show the PAH carbon turnover mass balance in microcosms with soil-compost mixtures or in farmyard fertilized soil using [(13)C6]-pyrene as a model PAH. Complete pyrene degradation of 100 mg/kg of soil was observed in all supplemented microcosms within 3 to 5 months, and the residual (13)C was mainly found as carbon converted to microbial biomass. Long-term fertilization of soil with farmyard manure resulted in pyrene removal efficiency similar to compost addition, although with a much longer lag phase, higher mineralization, and lower carbon incorporation into the biomass. Organic amendments either as long-term manure fertilization or as compost amendment thus play a key role in increasing the PAH-degrading potential of the soil microbial community. Phospholipid fatty acid stable isotope probing (PLFA-SIP) was used to trace the carbon within the microbial population and the amount of biomass formed from pyrene degradation. The results demonstrate that complex microbial degrader consortia rather than the expected single key players are responsible for PAH degradation in organic-amended soil.

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“…The lengths of lag times are often used to express the degree of adaptation to degradation of organic contaminants (Macleod & Semple, 2002;Reid et al, 2002;Lee et al, 2003). It has previously been suggested that microbial adaptation leads to a reduction in the lag phase and consequently faster rates of degradation (Gonod et al, 2003).…”

Section: Sterile Controlmentioning

confidence: 99%

“…Several authors have stated that increasing soil-contaminant contact time ('ageing') allows the adaptation of microbial communities to degrade specific contaminants (Spain et al, 1980;Spain & VanVeld, 1983;Misra et al, 1996;Carmichael & Pfaender, 1997;Hwang & Cutright, 2002;Macleod & Semple, 2002;Reid et al, 2002;Lee et al, 2003). For example, Macleod & Semple (2002) and Reid et al (2002) reported increased catabolic behaviour in soils towards pyrene and phenanthrene, respectively, with increased soil-PAH contact time. In this study, [UL-14 C] 2,4-dichlorophenol degradation was significantly greater in 4-week aged soils; degradation began without an acclimation period and plateaued after c. 5 days, although this varied between treatments.…”

Section: Sterile Controlmentioning

confidence: 99%

Biodegradation of 2,4-dichlorophenol in the presence of volatile organic compounds in soils under different vegetation types

Rhodes

Owen

Semple

2007

FEMS Microbiology Letters

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It has been suggested that monoterpenes emitted within the soil profile, either by roots or by decaying biomass, may enhance the biodegradation of organic pollutants. The aim of this study was to evaluate the effect of biogenic volatile organic compounds (VOCs) on the catabolism of 2,4-dichlorophenol in soils. Soils were collected from areas surrounding monoterpene (woodland) and nonmonoterpene (grassland)-emitting vegetation types. Soils were spiked with [UL-14C] 2,4-dichlorophenol at 10 mg kg(-1) and amended with alpha-pinene, p-cymene or a mix of monoterpenes (alpha-pinene, limonene and p-cymene in 1:1:1 ratio). The effects of monoterpene addition on the catabolism of [UL-14C] 2,4-dichlorophenol to 14CO2 by indigenous soil microbial communities were assessed in freshly spiked and 4-week-aged soils. It was found that aged woodland soils exhibited a higher level of [UL-14C] 2,4-dichlorophenol degradation, which was subsequently enhanced by the addition of monoterpenes (P<0.001), with the VOC mix and alpha-pinene amendments showing increased [UL-14C] 2,4-dichlorophenol catabolism. This study supports claims that the addition of biogenic VOCs to soils enhances the degradation of xenobiotic contaminants.

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Induction of PAH-catabolism in mushroom compost and its use in the biodegradation of soil-associated phenanthrene

Cited by 60 publications

References 22 publications

Molecular diversity analysis and bacterial population dynamics of an adapted seawater microbiota during the degradation of Tunisian zarzatine oil

Molecular diversity analysis and bacterial population dynamics of an adapted seawater microbiota during the degradation of Tunisian zarzatine oil

Degradation of 13C-labeled pyrene in soil-compost mixtures and fertilized soil

Biodegradation of 2,4-dichlorophenol in the presence of volatile organic compounds in soils under different vegetation types

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