Induction of Pathogen Resistance in Barley by Abiotic Stress

Wiese, Joachim; Kranz, Thorsten M.; Schubert, Sven

doi:10.1055/s-2004-821176

Cited by 115 publications

(69 citation statements)

References 39 publications

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“…In addition, Mohr and Cahill (2003) reported that treatment of Arabidopsis with ABA conferred partial loss of resistance against an avirulent isolate of P. syringae pv tomato. Contrary to these reports, Wiese et al (2004) recently described a positive role for ABA in pathogen resistance. The authors showed that treatment of barley with ABA primed for papillae-mediated resistance against powdery mildew fungus (Blumeria graminis f sp hordei ).…”

Section: Aba Regulates Baba-induced Protection Against Salt Stress Anmentioning

confidence: 67%

Dissecting the β-Aminobutyric Acid–Induced Priming Phenomenon in Arabidopsis

et al. 2005

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Plants treated with the nonprotein amino acid β-aminobutyric acid (BABA) develop an enhanced capacity to resist biotic and abiotic stresses. This BABA-induced resistance (BABA-IR) is associated with an augmented capacity to express basal defense responses, a phenomenon known as priming. Based on the observation that high amounts of BABA induce sterility in Arabidopsis thaliana, a mutagenesis screen was performed to select mutants impaired in BABA-induced sterility (ibs). Here, we report the isolation and subsequent characterization of three T-DNA–tagged ibs mutants. Mutant ibs1 is affected in a cyclin-dependent kinase–like protein, and ibs2 is defective in AtSAC1b encoding a polyphosphoinositide phosphatase. Mutant ibs3 is affected in the regulation of the ABA1 gene encoding the abscisic acid (ABA) biosynthetic enzyme zeaxanthin epoxidase. To elucidate the function of the three IBS genes in plant resistance, the mutants were tested for BABA-IR against the bacterium Pseudomonas syringae pv tomato, the oomycete Hyaloperonospora parasitica, and BABA-induced tolerance to salt. All three ibs mutants were compromised in BABA-IR against H. parasitica, although to a different extent. Whereas ibs1 was reduced in priming for salicylate (SA)-dependent trailing necrosis, mutants ibs2 and ibs3 were affected in the priming for callose deposition. Only ibs1 failed to express BABA-IR against P. syringae, which coincided with a defect in priming for SA-inducible PR-1 gene expression. By contrast, ibs2 and ibs3 showed reduced BABA-induced tolerance to salt, which correlated with an affected priming for ABA-inducible gene expression. For all three ibs alleles, the defects in BABA-induced sterility and BABA-induced protection against P. syringae, H. parasitica, and salt could be confirmed in independent mutants. The data presented here introduce three novel regulatory genes involved in priming for different defense responses.

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Section: Aba Regulates Baba-induced Protection Against Salt Stress Anmentioning

confidence: 67%

Dissecting the β-Aminobutyric Acid–Induced Priming Phenomenon in Arabidopsis

et al. 2005

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“…For example, both salt stress and exogenous ABA enhance the resistance of barley (Hordeum vulgare) to the biotrophic powdery mildew fungus Blumeria graminis (Wiese et al, 2004). Likewise, ABA application enhances resistance of Arabidopsis to the oomycete Pythium irregulare, while ABA-deficient and ABA-insensitive mutants are more susceptible (Adie et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Powdery Mildew Resistance Conferred by Loss of the ENHANCED DISEASE RESISTANCE1 Protein Kinase Is Suppressed by a Missense Mutation in KEEP ON GOING, a Regulator of Abscisic Acid Signaling

et al. 2008

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Loss-of-function mutations in the Arabidopsis (Arabidopsis thaliana) ENHANCED DISEASE RESISTANCE1 (EDR1) gene confer enhanced resistance to infection by powdery mildew (Golovinomyces cichoracearum). EDR1 encodes a protein kinase, but its substrates and the pathways regulated by EDR1 are unknown. To identify components of the EDR1 signal transduction pathway(s), we conducted a forward genetic screen for mutations that suppressed edr1-mediated disease resistance. Genetic mapping and cloning of one of these suppressor mutations revealed a recessive missense mutation in the KEEP ON GOING gene (KEG; At5g13530), which we designated keg-4. KEG encodes a multidomain protein that includes a RING E3 ligase domain, a kinase domain, ankyrin repeats, and HERC2-like repeats. The KEG protein has previously been shown to have ubiquitin ligase activity and to negatively regulate protein levels of the transcription factor ABCISIC ACID INSENSITIVE5. KEG mRNA levels were found to be 3-fold higher in edr1 mutant plants compared to wild type. Loss-of-function mutations in KEG are seedling lethal and are hypersensitive to glucose and abscisic acid (ABA). The keg-4 mutation, in contrast, conferred resistance to 6% glucose and suppressed edr1-mediated hypersensitivity to ABA, suggesting that the keg-4 mutation suppresses ABA signaling by altering KEG function. Several ABA-responsive genes were found to be further up-regulated in the edr1 mutant following ABA treatment, and this up-regulation was suppressed by the keg-4 mutation. We conclude that edr1-mediated resistance to powdery mildew is mediated, in part, by enhanced ABA signaling.

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“…Treatment of barley roots with ABA activated resistance in response to Blumeria graminis f. sp. hordei, and the pustule number was reduced by more than 50% [39]. Furthermore, the exogenous application of ABA-induced resistance of Arabidopsis against Alternaria brassicicola and Plectosphaerella cucumerina in wild-type plants [36].…”

Section: Introductionmentioning

confidence: 99%