Induction of peritoneal adhesions with small intestinal ischaemia and distention in the foal

Abstract

Summary Twenty‐two foals were divided into groups of intestinal distension and intestinal ischaemia as methods to induce peritoneal adhesions. In the first group, the lumen of a segment of distal small intestine was occluded without extramural vascular compromise and distended with lactated Ringer's solution to a constant pressure of 25 cm H2O for 2 h within the abdomen. The ischaemic group underwent 70 mins total vascular occlusion of identical segments of bowel. Serosal biopsies were obtained before and after each experimental procedure and following 60 mins of reperfusion. Similar biopsies were harvested from a control group of foals with no bowel occlusions. The foals were destroyed 10 days after surgery and tissues collected for histological and ultrastructural evaluation. Experimental and control mesothelial surfaces were denuded histologically immediately after experimental occlusions. Serosal oedema and cellular infiltration were observed following reperfusion of the ischaemic segments but were present immediately after 2 h of distension. All foals had developed bowel‐to‐bowel and bowel‐to‐mesentery adhesions of the experimental segments. Control foals under 30 days old exhibited mesenteric contraction and thickening of the isolated segment whereas those older than 30 days had little or no mesenteric thickening or contraction. Histologically, in the experimental segments, fibrous tissue had formed on the outer boundary of the original serosa, and new mesothelial‐like cells were present on the surface of fibrous tissue in some areas. Some serosal fibrosis was also seen in most of the control segments.

Indications, complications, and outcome of horses undergoing repeated celiotomy within 14 days after the first colic surgery: 95 cases (2005–2013)

“…Arguments for early intervention with a second celiotomy include reduction of ongoing daily costs for supportive therapy and avoiding the deleterious effects of continued small intestinal distension. [14][15][16][17][18] However, because there is a chance of spontaneous resolution of postoperative ileus and considering cost and morbidities associated with a second celiotomy, surgical intervention might be delayed for 48 to 72 hours, depending on surgeons' preference. In a study 19 investigating postoperative ileus after small intestinal surgery, mean duration of postoperative ileus was 64 ± 58 hours (median, 40 hours; range, 1 to 240 hours).…”

Avaliação histomorfométrica e ultra-estrutural da mucosa do cólon menor eqüino submetido a distensão

“…Experimentos realizados com distensão intraluminal do jejuno de eqüinos também revelaram lesões discretas na mucosa e lesões mais evidentes na seromuscular (Allen et al 1988, Lundin et al 1989, Parks et al 1989, Dabareiner et al 1993, 2001a. Este achado poderia ser explicado pelo tipo de constituição tecidual das camadas.…”

“…Vários estudos têm demonstrado, de formas clínica e experimental, que o aumento de pressão intraluminal, e conseqüente distensão, têm associação direta com o prognóstico e com complicações pós-operatórias como aderências (Lundin et al 1989) e íleo paralítico (Malone & Kannan 2001).…”

Deneysel ileusda indometasin kullanımının intestinal permeabilite ve bakteriyel translokasyon üzerine etkileri The effects ofindomethacine on intestinal permeability and bacterial translocation in in

“…Enjeksiyonları izleyerek hayvanlar tek tek metabolik kafeslere yerleştirildiler ve sıvı kısıtlaması yapıl-maksızın 6 saat süresince idrar örnekleri toplandı. Her bir hayvanın, oral yolla verilen radyoaktif 51 Cr EDTA yı yüzde kaç oranında idrarla attığının belirlenebilmesi için idrar örneklerin içerdiği radyoaktivite gama sayacında ölçüldü. Ölçümler Ankara Üniversitesi Nükleer Tıp Anabilim Dalı Laboratuvarında DPC Gambyt CR (95-3/1160-1992) cihazı ile gerçekleştirildi.…”

“…Intestinal obstruction period and indomethacine dosages were same as in group II and III, respectively. Intestinal mucosal permeability to 51 Cr labeled ethylen diamin tetraacetic acid ( 51 Cr EDTA) was measured by collecting urine samples in metabolic cages for six hours following 24 hours of intestinal obstruction period. After six hours re-laparatomy was performed.…”