Induction of Phenylalanine Ammonia-Lyase Activation and Isoflavone Glucoside Accumulation in Suspension-Cultured Cells of Red Bean, Vigna angularis, by Phytoalexin Elicitors, Vanadate, and Elevation of Medium pH

Hattori, Tsukaho; Ohta, Yoshimoto

doi:10.1093/oxfordjournals.pcp.a077006

Cited by 49 publications

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“…Nigericin showed the same kinetics as orthovanadate in increasing PAL activity. The effect of these elicitors is slower than that ofbiotic elicitors, which is in accordance with earlier work (10,12,26). Increase in PAL activity is generally due to de novo synthesis of the enzyme ( 19).…”

supporting

confidence: 91%

“…Inhibition of the plasma membrane ATPase by orthovanadate would reduce the proton electrochemical gradient across this membrane. This idea has been corroborated by the finding that an external pH shift can give rise to elicitation also (10,12). The proton gradient consists of a ApH (acidic outside) and a At (positive outside).…”

mentioning

confidence: 78%

“…This has been done by addition of nigeran, a polyglucan isolated from Aspergillus niger, and orthovanadate, an abiotic elicitor (see also ref. 12). Changes in the activity of the PPP were followed by measuring the activity of the enzyme PAL, a key enzyme of the general part of this pathway.…”

mentioning

confidence: 99%

“…The elicitor is recognized by and bound to a specific receptor protein on the plasma membrane (23). Then it has been suggested that inhibition of the plasma membrane ATPase is the next step in elicitation (12) because orthovanadate, an inhibitor of plasma membrane ATPases (3), can act as an elicitor as well (10,12). Inhibition of the plasma membrane ATPase by orthovanadate would reduce the proton electrochemical gradient across this membrane.…”

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confidence: 99%

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Effect of Elicitors on the Plasmamembrane of Petunia hybrida Cell Suspensions

Hagendoorn¹,

Poortinga²,

Sang³

et al. 1991

Plant Physiol.

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Primary processes during elicitation of the phenylpropanoid pathway (PPP) were studied in Petunia hybrida cell suspensions. We tested the hypothesis that decrease of the proton gradient across the plasma membrane activates the PPP. Induction of the PPP was determined by measuring phenylalanine ammonia Iyase activity. A variety of ATPase inhibitors and ionophores were tested for the ability to elicit the PPP. The ATPase inhibitors orthovanadate and NN'-dicyclohexylcarbodiimide and the ionophores carbonyl cyanide-4-trifluoromethoxyphenylhydrazone and nigericin were all effective elicitors. Carbonyl cyanide-4-trifluoromethoxyphenylhydrazone and nigericin elicit also when used in combination with NN'-dicyclohexylcarbodiimide. Valinomycin had little effect on phenylalanine ammonia Iyase activity. Treatment with orthovanadate or nigericin led to the formation of lignin. Alkalinization of the external medium by NN'-dicyclohexylcarbodiimide, carbonyl cyanide-4-trifluoromethoxyphenylhydrazone, and nigericin was observed directly with the use of a sensitive pH electrode and internal acidification was deduced from the changes in emission intensity of the fluorescent probe bis [3-propyl-5-oxoisoxazol-4-yl] pentamethineoxonol. These data indicate that changes in the activity of the plasmamembrane H+-ATPase, and subsequent decrease of the proton gradient (particularly of the pH gradient) by itself are sufficient to influence phenylalanine ammonia Iyase activity of P. hybrida cells and are therefore important intermediates in signal transduction.In plant cell and tissue cultures, generally no significant production of secondary metabolites is observed. To initiate this production, many strategies have been followed, such as treatment with so-called elicitors (see ref. 8 and references therein). For example, biotic elicitors, isolated from plant pathogens, induced the production of isoflavonoid phytoalexins in soybean (7). Also abiotic elicitors, such as high medium osmolarity (15), are reported to induce the production of secondary metabolites.

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confidence: 91%

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confidence: 78%

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confidence: 99%

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confidence: 99%

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Effect of Elicitors on the Plasmamembrane of Petunia hybrida Cell Suspensions

Hagendoorn¹,

Poortinga²,

Sang³

et al. 1991

Plant Physiol.

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“…Abiotic stress factors like CuC12 or UV light (Munn and Drysdale 1975) as well as biotic substances like an elicitor from cell walls of Phytophthora megasperma var. sojae (Hattori and Ohta 1985) can induce PAL activity in Viona cell-suspension cultures. In these cases, the induction of PAL was accompanied by phytoalexin synthesis, and Ralton et al (1988) report a correlation between induced PAL activity and resistance of different V. sinensis varieties to Phytophthora vignae.…”

Section: Introductionmentioning

confidence: 96%

Early defence responses of cowpea (Vigna sinensis L.) induced by non-pathogenic rust fungi

Fink

Haug

Deising

et al. 1991

Planta

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Abstract. In cowpea (Vigna s&ensis L.) leaves the pathogenic rust fungus Uromyces vignae and the non-pathogens U. appendiculatus and U. viciae-fabae developed similarly to give rise to more than 80% haustorial mother cells. Whereas U. vignae was able to sporulate, the nonpathogens were stopped either after formation of some haustoria (U. appendiculatus) or immediately after formation of haustorial mother cells (U. viciae-fabae).Approximately 30% of the cells in contact with haustorial mother cells of the two non-pathogens showed autofluorescence and deposition of phloroglucinol/HC1-positive material. The early defence reactions of V. sinensis include induction of phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) and extracellular peroxidase (POD, EC 1.11.1.7) activity as early as 10h and 24h after inoculation, respectively. Probing Western blots with heterologous monospecific anti-PAL serum showed that pathogenesis-induced increases in enzyme activity are the result of de novo synthesis. Native polyacrylamide gel electrophoresis revealed the specific induction of two extracellular acidic POD forms in cowpea inoculated with the non-pathogens. Both PAL and apoplasmic POD activities were highest in U. viciae-fabae-, intermediate in U. appendiculatus-and low in U. vignae-inoculated or talcum-treated control plants. The timing of increasing PAL and extracellular POD activities in relation to deposition of lignin or lignin-like material in mesophyll cell walls indicates the involvement of lignification in the early defence of V. sinensis against biotrophic fungal parasites. Analysis of the substrate specificity of the inducible POD forms, separated and partially purified by chromatofocusing, showed that apoplasmic PODs preferentially oxidize the naturally occurring substrate coniferyl alcohol. These results support the assumption that POD-mediated lignification is involved in the expression of non-host resistance of cowpea to rust fungi. * To whom correspondence should be addressed Abbreviations: IWF = intercellular washing fluid; PAL = phenylalanine ammonia-lyase; p.i. = post inoculation ; POD = peroxidase; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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2020

Secondary Metabolites of Medicinal Plants

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Induction of Phenylalanine Ammonia-Lyase Activation and Isoflavone Glucoside Accumulation in Suspension-Cultured Cells of Red Bean, Vigna angularis, by Phytoalexin Elicitors, Vanadate, and Elevation of Medium pH

Cited by 49 publications

References 0 publications

Effect of Elicitors on the Plasmamembrane of Petunia hybrida Cell Suspensions

Effect of Elicitors on the Plasmamembrane of Petunia hybrida Cell Suspensions

Early defence responses of cowpea (Vigna sinensis L.) induced by non-pathogenic rust fungi

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