Induction of Plantlets from Anthers of^|^lsquo;Trovita^|^rsquo;Orange

Abstract

SummaryAnther culture of `Trovita' orange, a cultivar of sweet orange (Citrus sinensis Osbeck), was attempted.Anthers with microspores at uninucleate stage were cultured on the Murashige and Skoog's medium(9) supplemented with or without indole-3-acetic acid (IAA) and kinetin at 28°C in darkness.Embryoids appeared directly from within anthers after about 10 weeks of culture.The media containing kinetin and IAA both at 0.002 or 0.02 mg/1 were effective in embryoid formation.In addition, 0.2 or 2.0 mg/1 kinetin and 0.2 mg/1 IAA seemed to be also favorable when combined with 0.002 or 0.02 mg/1 IAA and 0.002 or 0.02 mg/1 kinetin, respectively.Calli were formed well on the media with kinetin at 2.0 mg/1 followed by those with IAA at 2.0 mg/l.After incubation in light with a 16-hour photoperiod, some embryoids differentiated shoots.Induction of roots was stimulated by transfer of embryoids from the embryoid induction media containing growth regulators to a medium without them.Some root tip cells of differentiated plantlets had a diploid chromosome number of 18.

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

“…In particular, anther culture has produced somatic regenerants in C. aurantium (Hidaka et al 1981;Germanà 2005); C. sinensis (Hidaka 1984); C. aurantifolia (Chaturvedi & Sharma 1985); C. madurensis (Ling et al 1988); C. reticulata (Germanà et al 1994;Germanà 2005); P. trifoliata, and the hybrid 14 of C. ichangensis £ C. reticulata (Deng et al 1992). In these cases, anther culture can be regarded as an additional method for achieving somatic embryogenesis.…”