Induction of precocious females in the protandrous barramundi (Lates calcarifer) through implants containing 17β-estradiol - effects on gonadal morphology, gene expression and DNA methylation of key sex genes

Banh, Quyen Q.; Guppy, Jarrod L.; Domingos, Jose A.; Budd, Alyssa M.; Pinto, Ricardo Camurça Correia; Marc, Adrien F.; Jerry, Dean R.

doi:10.1016/j.aquaculture.2021.736601

Cited by 17 publications

(12 citation statements)

References 131 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, while DNAm can cause changes in hormone production and hormone receptor expression, hormones can also cause changes in DNAm, for example, through the regulation of their associated enzymes (DNMTs; Baumbach & Zovkic, 2020). In barramundi specifically, estrogen treatment resulted in decreased DNAm and increased expression of cyp19a1a , as well as early male‐to‐female sex change (Banh et al, 2021). It remains unknown whether changes in DNAm occur prior to or following changes in gene expression and natural hormone production, which lead to sex change in fish.…”

Section: Discussionmentioning

confidence: 99%

“…To analyze DNAm levels of the region surrounding the start codon of cyp19a1a , esr1 , dmrt1 , and nr5a2 , a targeted bisulphite amplicon sequencing (BSAS) approach, adapted from Masser et al (2013), was applied. These regions were selected based on our previous work, both published ( cyp19a1a and nr5a2 ) and unpublished ( esr1 and dmrt1 ), to identify those showing significant correlations with phenotypic sex (Banh et al, 2021; Budd, 2020; Domingos et al, 2018). While the region surrounding the transcription start site is typically the most informative (Piferrer et al, 2019), not all sex‐related genes show sex‐specific DNAm patterns in amplicons near this region (e.g., sox9 ; Figure ).…”

Section: Methodsmentioning

confidence: 99%

Section: Bisulphite Conversion Of Gdna and Amplicospecific Pcrmentioning

confidence: 99%

See 2 more Smart Citations

Epigenetics underpins phenotypic plasticity of protandrous sex change in fish

Budd

Robins

Whybird³

et al. 2022

Ecology and Evolution

Self Cite

View full text Add to dashboard Cite

Phenotypic plasticity is an important driver of species resilience. Often mediated by epigenetic changes, phenotypic plasticity enables individual genotypes to express variable phenotypes in response to environmental change. Barramundi (Lates calcarifer) are a protandrous (male‐first) sequential hermaphrodite that exhibits plasticity in length‐at‐sex change between geographic regions. This plasticity is likely to be mediated by changes in DNA methylation (DNAm), a well‐studied epigenetic modification. To investigate the relationships between length, sex, and DNAm in a sequential hermaphrodite, here, we compare DNAm in four conserved vertebrate sex‐determining genes in male and female barramundi of differing lengths from three geographic regions of northern Australia. Barramundi first mature as male and later sex change to female upon the attainment of a larger body size; however, a general pattern of increasing female‐specific DNAm markers with increasing length was not observed. Significant differences in DNAm between males and females of similar lengths suggest that female‐specific DNAm arises rapidly during sex change, rather than gradually with fish growth. The findings also reveal that region‐specific differences in length‐at‐sex change are accompanied by differences in DNAm and are consistent with variability in remotely sensed sea temperature and salinity. Together, these findings provide the first in situ evidence for epigenetically and environmentally mediated sex change in a protandrous hermaphrodite and offer significant insight into the molecular and ecological processes governing the marked and unique plasticity of sex in fish.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Bisulphite Conversion Of Gdna and Amplicospecific Pcrmentioning

confidence: 99%

See 1 more Smart Citation

Epigenetics underpins phenotypic plasticity of protandrous sex change in fish

Budd

Robins

Whybird³

et al. 2022

Ecology and Evolution

Self Cite

View full text Add to dashboard Cite

show abstract

“…Steroid hormones are natural and induce sex change processes [ 50 ], among which E2 is one of the most potent estrogens impacting sex differentiation [ 51 ] and MT is one of the most potent androgens impacting sex differentiation [ 52 ]. For instance, E2 induces sex reversal in P. sinensis and the protandrous barramundi ( Lates calcarifer ) [ 53 , 54 ], and MT controls secondary sexual characteristics in Siniperca chuatsi as well [ 55 ]. The same phenomenon has been confirmed in mammals and fish [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Expression and Characterization of the Spats1 Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Luo

Zhu

Chen

et al. 2022

Animals

View full text Add to dashboard Cite

Spats1 (spermatogenesis-associated, serinerich 1) has been characterized as a male-biased gene which acts an important role in the germ cell differentiation of mammals. Nevertheless, the function of Spats1 in the Chinese soft-shelled turtle (P. sinensis) has not yet been reported. To initially explore the expression of Spats1 in P. sinensis and its response to sex steroid treatment, we cloned the CDS of Spats1 for the first time and analyzed its expression profile in different tissues, including the testes in different seasons. The Spats1 cDNA fragment is 1201 base pairs (bp) in length and contains an open reading frame (ORF) of 849 bp, which codes for 283 amino acids. Spats1 mRNA was highly expressed in the testes (p < 0.01) and barely detectable in other tissues. In P. sinensis, the relative expression of Spats1 also responsive to seasonal changes in testis development. In summer (July) and autumn (October), Spats1 gene expression was significantly higher in the testes than in other seasons (p < 0.05). Spats1 mRNA was found to be specifically expressed in germ cells by chemical in situ hybridization (CISH), and it was mainly located in primary spermatocytes (Sc1), secondary spermatocytes (Sc2) and spermatozoa (St). Spats1 expression in embryos was not significantly changed after 17α-methyltestosterone (MT)and 17β-estradiol (E2) treatment. In adults, MT significantly induced Spats1 expression in male P. sinensis. However, the expression of Spats1 in testes was not responsive to E2 treatment. In addition, the expression of Spats1 in females was not affected by either MT or E2 treatment. These results imply that Spats1 is a male-specific expressed gene that is mainly regulated by MT and is closely linked to spermatogenesis and release in P. sinensis.

show abstract

“…The experimental results suggest that estradiol treatment induced the upregulation of CsFOXL2. It indicates that estradiol may play a role in ovarian development by stimulating FOXL2 to induce estrogenic feedback mechanism (Narisato et al, 2006;Li et al, 2014;Banh Quyen et al, 2021). However, the expression level of CsFOXL2 was decreased at estradiol concentration of 50 µg/L compared with estradiol concentration of 5 µg/L.…”

mentioning

confidence: 93%

Characterization, expression profiling, and estradiol response analysis of DMRT3 and FOXL2 in clam Cyclina sinensis

Yan¹,

Xu²,

Xie³

et al. 2023

Front. Mar. Sci.

View full text Add to dashboard Cite

The clam Cyclina sinensis is one of the important economical aquaculture shellfish in China. However, the mechanisms of sex determination and differentiation in C. sinensis have not been fully studied. In this study, full-length cDNAs of DMRT3 and FOXL2 were cloned and functionally characterized. The ORF region of CsDMRT3 consists of 1137 nucleotides, which encode 378 amino acids contains a conserved DM domain of DMRT family. The ORF region of CsFOXL2 is 1245 bp, encodes 414 amino acids, and contains a conserved FH domain. Tissue-specific expression results showed that the higher expression level of CsDMRT3 and CsFOXL2 was found in the ovary and testis of C. sinensis. The expression levels of CsDMRT3 and CsFOXL2 also peaked at the maturation stage of male and female gonadal development, respectively. Moreover, the expression levels of CsDMRT3 and CsFOXL2 were significantly higher in the trochophore and D-larval stages than in other stages. The transcript levels of CsDMRT3 reached the highest level at 11 months of age, while the CsFOXL2 reached the highest level at 7 months of age. In estradiol-treated experiments, the expression levels of CsDMRT3 and CsFOXL2 in the gonads were highest at 5 µg/L estradiol treatment, and histologically, it was observed that the oocytes diameters became larger with increasing estradiol concentration. These results suggest that CsDMRT3 and CsFOXL2 play an important role in gonadal development and sex differentiation of C. sinensis.

show abstract

Induction of precocious females in the protandrous barramundi (Lates calcarifer) through implants containing 17β-estradiol - effects on gonadal morphology, gene expression and DNA methylation of key sex genes

Cited by 17 publications

References 131 publications

Epigenetics underpins phenotypic plasticity of protandrous sex change in fish

Epigenetics underpins phenotypic plasticity of protandrous sex change in fish

Expression and Characterization of the Spats1 Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Characterization, expression profiling, and estradiol response analysis of DMRT3 and FOXL2 in clam Cyclina sinensis

Contact Info

Product

Resources

About