Induction of programmed cell death (apoptosis) in mature lymphocytes

Lucas, Miguel; Solano, Francisca; Sanz, Aureo

doi:10.1016/0014-5793(91)80239-y

Cited by 28 publications

(12 citation statements)

References 14 publications

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“…A well-studied model is induction of thymocyte apoptosis by exogenously administered glucocorticoids [38]. Most of this work has been done in mammals using rodent [13-151 or human [39] lymphocytes. Until recently, little data were available for the avian system.…”

Section: Discussionmentioning

confidence: 99%

Thymocyte apoptosis induced by elevated endogenous corticosterone levels

et al. 1994

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A well-known model of apoptosis is induction in thymocytes by injection of pharmacological doses of exogenous steroids. The aim of this study was to investigate whether this process also occurs under physiological conditions, i.e. by stimulation of endogenous glucocorticoid release, using the chicken as an experimental model. Endogenous glucocorticoid levels can be elevated by immunization with exogenous antigens or by injection of conditioned medium, e.g. supernatant of mitogen-stimulated spleen cells. This effect is mediated by so-called glucocorticoid-increasing factors, and is considered to act as an immunoregulatory principle. Thymocyte DNA of so treated birds showed a typical "ladder" pattern after electrophoresis in a 1.8% agarose gel, and degradation could be prevented by RU 38,486. This provides evidence that apoptosis can be induced by elevating endogenous corticosterone levels in vivo. By means of in situ nick translation (ISNT) and simultaneous immunofluorescence tests, it was possible to analyze various thymic subpopulations during apoptosis after treatment with exogenous glucocorticoids. Additionally, using confocal microscopical techniques, apoptosis of the same cells as analyzed by ISNT is shown. The possible role of elevated concentrations of endogenous glucocorticoids in regulating thymocyte cell death and autoimmune diseases is discussed.

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Section: Discussionmentioning

confidence: 99%

Thymocyte apoptosis induced by elevated endogenous corticosterone levels

et al. 1994

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“…These include uterine epithelium (51), prostate (50), erythroid progenitor cells (54), keratinocytes (48), peripheral blood lymphocytes (104), isolated leukemia cells (70, 71), macrophages (88), and the targets of cytotoxic T lymphocytes and natural killer cells (105). Since the initial discovery of internucleosomal DNA cleavage in glucocorticoid-treated thymocytes, this process has been observed in almost all of the instances of apoptosis that have been studied.…”

Section: A Chromatin Degradationmentioning

confidence: 99%

Apoptosis: The Biochemistry and Molecular Biology of Programmed Cell Death*

1993

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“…The life-span of the lymphocyte includes a ®nal step in the secondary lymphoid tissue. Many cells survive and the rest are committed to apoptotic cell death [9,23]. Activation of an endogenous endonuclease has been described associated with apoptosis in many cell types.…”

Section: Introductionmentioning

confidence: 99%