Induction of proliferation of basal epidermal keratinocytes by cold atmospheric-pressure plasma

Hasse, Sybille; Tran, Tri; Hahn, Olga; Kindler, Stefan; Metelmann, H.‐R.; Woedtke, Thomas von; Masur, Kai

doi:10.1111/ced.12735

Cited by 103 publications

(87 citation statements)

References 24 publications

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“…This is in agreement with the literature where no histological damage was observed in human skin after 1 min [58] or 10 min of plasma treatment [59]. The absence of expression of a panel of tumor markers in liver, lung, brain, and ear tissue using qPCR suggests that plasma can support wound healing [45] without alterations in the tissue architecture and/or function.…”

Section: Discussionsupporting

confidence: 91%

One Year Follow-Up Risk Assessment in SKH-1 Mice and Wounds Treated with an Argon Plasma Jet

Schmidt

Woedtke

Stenzel³

et al. 2017

IJMS

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Multiple evidence in animal models and in humans suggest a beneficial role of cold physical plasma in wound treatment. Yet, risk assessment studies are important to further foster therapeutic advancement and acceptance of cold plasma in clinics. Accordingly, we investigated the long-term side effects of repetitive plasma treatment over 14 consecutive days in a rodent full-thickness ear wound model. Subsequently, animals were housed for 350 days and sacrificed thereafter. In blood, systemic changes of the pro-inflammatory cytokines interleukin 1β and tumor necrosis factor α were absent. Similarly, tumor marker levels of α-fetoprotein and calcitonin remained unchanged. Using quantitative PCR, the expression levels of several cytokines and tumor markers in liver, lung, and skin were found to be similar in the control and treatment group as well. Likewise, histological and immunohistochemical analysis failed to detect abnormal morphological changes and the presence of tumor markers such as carcinoembryonic antigen, α-fetoprotein, or the neighbor of Punc 11. Absence of neoplastic lesions was confirmed by non-invasive imaging methods such as anatomical magnetic resonance imaging and positron emission tomography-computed tomography. Our results suggest that the beneficial effects of cold plasma in wound healing come without apparent side effects including tumor formation or chronic inflammation.

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Section: Discussionsupporting

confidence: 91%

One Year Follow-Up Risk Assessment in SKH-1 Mice and Wounds Treated with an Argon Plasma Jet

Schmidt

Woedtke

Stenzel³

et al. 2017

IJMS

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“…Daily short plasma applications (up to 40 s) to murine superficial skin wounds showed significantly increased epidermal cell regeneration, granulation tissue hyperplasia, and collagen deposition [93]. This was also confirmed by plasma application on human skin biopsies where short CAP exposure (1-3 min) was already able to induce the proliferation of keratinocytes [94]. Concomitantly, longer exposures might lead to overdosing of the plasma application inducing apoptotic cell death with a further disturbed wound healing process [93,94].…”

Section: Plasmamentioning

confidence: 87%

“…This was also confirmed by plasma application on human skin biopsies where short CAP exposure (1-3 min) was already able to induce the proliferation of keratinocytes [94]. Concomitantly, longer exposures might lead to overdosing of the plasma application inducing apoptotic cell death with a further disturbed wound healing process [93,94]. Chernets et al [95] examined appendage regeneration stimulated by NTP dependent on reactive oxidative species.…”

Section: Plasmamentioning

confidence: 90%

Skin Wound Healing: An Update on the Current Knowledge and Concepts

Sorg

Tilkorn²,

Hager³

et al. 2016

Eur Surg Res

948

691

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Background: The integrity of healthy skin plays a crucial role in maintaining physiological homeostasis of the human body. The skin is the largest organ system of the body. As such, it plays pivotal roles in the protection against mechanical forces and infections, fluid imbalance, and thermal dysregulation. At the same time, it allows for flexibility to enable joint function in some areas of the body and more rigid fixation to hinder shifting of the palm or foot sole. Many instances lead to inadequate wound healing which necessitates medical intervention. Chronic conditions such as diabetes mellitus or peripheral vascular disease can lead to impaired wound healing. Acute trauma such as degloving or large-scale thermal injuries are followed by a loss of skin organ function rendering the organism vulnerable to infections, thermal dysregulation, and fluid loss. Methods: For this update article, we have reviewed the actual literature on skin wound healing purposes focusing on the main phases of wound healing, i.e., inflammation, proliferation, epithelialization, angiogenesis, remodeling, and scarring. Results: The reader will get briefed on new insights and up-to-date concepts in skin wound healing. The macrophage as a key player in the inflammatory phase will be highlighted. During the epithelialization process, we will present the different concepts of how the wound will get closed, e.g., leapfrogging, lamellipodial crawling, shuffling, and the stem cell niche. The neovascularization represents an essential component in wound healing due to its fundamental impact from the very beginning after skin injury until the end of the wound remodeling. Here, the distinct pattern of the neovascularization process and the special new functions of the pericyte will be underscored. At the end, this update will present 3 topics of high interest in skin wound healing issues, dealing with scarring, tissue engineering, and plasma application. Conclusion: Although wound healing mechanisms and specific cell functions in wound repair have been delineated in part, many underlying pathophysiological processes are still unknown. The purpose of the following update on skin wound healing is to focus on the different phases and to brief the reader on the current knowledge and new insights. Skin wound healing is a complex process, which is dependent on many cell types and mediators interacting in a highly sophisticated temporal sequence. Although some interactions during the healing process are crucial, redundancy is high and other cells or mediators can adopt functions or signaling without major complications.

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“…Its sterilization effect does not depend on the pathogen species and can even resist multidrug-resistant bacteria [74] . Some reports indicate that NAP can enhance the proliferation rate of basal keratinocytes and endothelial cells [75,76] . The 350 downregulated and 400 upregulated transcripts of keratinocytes after NAP treatment highlighted its powerful ability to influence gene expression [77] .…”

Section: Hydrogen Peroxide: Implications For Chronic Wound Treatmentmentioning

confidence: 99%

Hydrogen Peroxide: A Potential Wound Therapeutic Target

Zhu¹,

Wang²,

Lu³

et al. 2017

Med Princ Pract

114

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Hydrogen peroxide (H2O2) is a topical antiseptic used in wound cleaning which kills pathogens through oxidation burst and local oxygen production. H2O2 has been reported to be a reactive biochemical molecule synthesized by various cells that influences biological behavior through multiple mechanisms: alterations of membrane potential, generation of new molecules, and changing intracellular redox balance, which results in activation or inactivation of different signaling transduction pathways. Contrary to the traditional viewpoint that H2O2 probably impairs tissue through its high oxidative property, a proper level of H2O2 is considered an important requirement for normal wound healing. Although the present clinical use of H2O2 is still limited to the elimination of microbial contamination and sometimes hemostasis, better understanding towards the sterilization ability and cell behavior regulatory function of H2O2 within wounds will enhance the potential to exogenously augment and manipulate healing.

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Induction of proliferation of basal epidermal keratinocytes by cold atmospheric-pressure plasma

Abstract: We investigated the effect of cold plasma on human skin by detecting molecules for growth and apoptosis, and found that both processes are dependent on treatment time. Therefore, this approach offers promising results for further applications of cold plasma in clinical dermatology.

Cited by 103 publications

References 24 publications

One Year Follow-Up Risk Assessment in SKH-1 Mice and Wounds Treated with an Argon Plasma Jet

One Year Follow-Up Risk Assessment in SKH-1 Mice and Wounds Treated with an Argon Plasma Jet

Skin Wound Healing: An Update on the Current Knowledge and Concepts

Hydrogen Peroxide: A Potential Wound Therapeutic Target

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