1992
DOI: 10.1083/jcb.116.4.1035
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Induction of proliferation or hypertrophy of chondrocytes in serum-free culture: the role of insulin-like growth factor-I, insulin, or thyroxine.

Abstract: Abstract. In bone forming cartilage in vivo, cells undergo terminal differentiation, whereas most of the cells in normal articular cartilage do not . Chondrocyte hypertrophy can be induced also in vitro by diffusible signals. We have identified growth factors or hormones acting individually on 17-d chick embryo sternal chondrocytes cultured in agarose gels under strictly serumfree conditions. Insulin-like growth factor I or insulin triggered the first steps of chondrocyte maturation, i.e., cell proliferation a… Show more

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Cited by 165 publications
(115 citation statements)
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“…Current data suggest that the two phases of chondrocyte growth are regulated separately; IGF-I stimulates proliferation, whereas T3 induces hypertrophic differentiation (115). The fact that T4 is also active in this system is noteworthy.…”
Section: Hormone Action In Skeletal Cellsmentioning
confidence: 95%
“…Current data suggest that the two phases of chondrocyte growth are regulated separately; IGF-I stimulates proliferation, whereas T3 induces hypertrophic differentiation (115). The fact that T4 is also active in this system is noteworthy.…”
Section: Hormone Action In Skeletal Cellsmentioning
confidence: 95%
“…They also postulated that insulin acts directly on chondrocyte maturation without utilizing the secondary pathway of binding to IGF-I receptors. Indeed, in vivo (17) but not in vitro (24), the local production of IGF-I is apparently necessary for the growth-promoting role of insulin. Quarto et al (25) also showed that FGF-2 induces chondrocyte proliferation if associated with insulin.…”
Section: Discussionmentioning
confidence: 99%
“…These doses of individual factors were within the range between effective dose 75 and effective dose 100 of their proper effects on chondrocytes, according to dose-response curves in the previous reports (11,12,15,16,(21)(22)(23)(24)(25)(26)(27)(28) cells in 5 l of 0.8% atelocollagen gel) were cultured in Dulbecco's modified Eagle's medium/F-12 containing the combinations for 2 weeks, and the GAG accumulation was measured as described below. Parameter estimates of the GAG accumulation by one factor or two were calculated from the F values that represent the effects of the individual factors and the interaction terms of two factors by the analysis of variance using the software above.…”
Section: Methodsmentioning
confidence: 99%