Induction of protective immunity by aerosol or oral application of candidate vaccines in a dose-controlled pig aerosol infection model

Hensel, Andreas; Leengoed, L.A.M.G. van; Szostak, Michael P.; Windt, H.; Weissenböck, Herbert; Stockhofe-Zurwieden, Norbert; Katinger, Astrid; Stadler, Maria; Ganter, Martin; Bunka, S; Pabst, Reinhard; Lubitz, Werner

doi:10.1016/0168-1656(95)00150-6

Cited by 44 publications

(27 citation statements)

References 31 publications

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“…BALT might have been the entry site for live or attenuated Actinobacillus pleuropneumoniae applied as a vaccine in aerosol form which protected the pigs from a considerable dose of these bacteria in an exposure [44]. After infection of pigs with Mycoplasma hyopneumonia, hyperplasia of BALT is the most significant change.…”

Section: Porcinementioning

confidence: 99%

“…Functional studies on lymphocyte traffic revealed major differences in the extent of immigrating lymphocytes: large numbers of lymphocytes immigrate into JPP, whereas little cell traffic is found in the IPP with the exception of the 10 to 20 cm next to the ileocecal junction where entry is as high as in JPP [87]. The protective effect of the orally applied lungspecific bacterium Actinobacillus pleuropneumoniae in a subsequent aerosol exposure of an LD 50 of this bacterium can be taken as an example of the integrated mucosal immune system and its relevance in future vaccine strategies in farm animals [44]. In a recently published study, four different subsets of dendritic cells were characterized in JPP, lamina propria, gut lymph and mesenteric lymph nodes of pigs, and the functional relevance is outlined for future vaccine studies [14].…”

Section: Histology and Lymphocyte Subsetsmentioning

confidence: 99%

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MALT structure and function in farm animals

2006

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-Mucosa-associated lymphoid tissue (MALT) is defined as an organized lymphoid tissue in the mucosa that samples antigens. The morphological characteristics that distinguish MALT from lymphoid infiltrates are discussed. MALT has been extensively investigated in laboratory animals, while knowledge in cattle, sheep, goats, pigs and horses that are summarized under the term farm animals in this review is fragmentary. Literature data about the distribution, morphology, function and involvement in infectious diseases of MALT in farm animals are described. The understanding of specific features of MALT in other species than laboratory animals is important for comparative research, in order to understand pathological and immunological processes in the respective species and as a potential route of vaccination of mucosal surfaces.

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Section: Porcinementioning

confidence: 99%

Section: Histology and Lymphocyte Subsetsmentioning

confidence: 99%

MALT structure and function in farm animals

2006

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“…They also prevented colonization of the lungs and tonsils which indicated that immunization with BGs is superior to treatment with bacterins. 43 More importantly, no clinical side-effects have been reported.…”

Section: Bgs-applicationsmentioning

confidence: 99%

“…The mucosal application of App BGs as oral immunization 44 or as aerosols 43,45 induced sterile immunity and cross-protection against other serotypes in pigs 45 whereas intramuscular immunization 46 fully prevented the vaccinated pigs against the disease after lethal challenge but did not confer sterile immunity because the challenge bacteria could be re-isolated from the tonsils from the vaccinated pigs.…”

Section: Bgs-applicationsmentioning

confidence: 99%

The bacterial ghost platform system

Langemann¹,

et al. 2010

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The Bacterial Ghost (BG) platform technology is an innovative system for vaccine, drug or active substance delivery and for technical applications in white biotechnology. BGs are cell envelopes derived from Gram-negative bacteria. BGs are devoid of all cytoplasmic content but have a preserved cellular morphology including all cell surface structures. Using BGs as delivery vehicles for subunit or DNA-vaccines the particle structure and surface properties of BGs are targeting the carrier itself to primary antigenpresenting cells. Furthermore, BGs exhibit intrinsic adjuvant properties and trigger an enhanced humoral and cellular immune response to the target antigen. Multiple antigens of the native BG envelope and recombinant protein or DNA antigens can be combined in a single type of BG. Antigens can be presented on the inner or outer membrane of the BG as well as in the periplasm that is sealed during BG formation. Drugs or supplements can also be loaded to the internal lumen or periplasmic space of the carrier. BGs are produced by batch fermentation with subsequent product recovery and purification via tangential flow filtration. For safety reasons all residual bacterial DNA is inactivated during the BG production process by the use of staphylococcal nuclease A and/or the treatment with β-propiolactone. After purification BGs can be stored long-term at ambient room temperature as lyophilized product. The production cycle from the inoculation of the pre-culture to the purified BG concentrate ready for lyophilization does not take longer than a day and thus meets modern criteria of rapid vaccine production rather than keeping large stocks of vaccines. The broad spectrum of possible applications in combination with the comparably low production costs make the BG platform technology a safe and sophisticated product for the targeted delivery of vaccines and active agents as well as carrier of immobilized enzymes for applications in white biotechnology.

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“…51 Pigs are a good model for human diseases and these studies encourage using BG aerosols also in humans (e.g., for Chlamydia pneumoniae) either as vaccine or drug carrier. The oral immunization of rabbits with V. cholerae ghosts induced protective immunity against O1 challenge in the RITARD model and conferred cross protection against the recently emerging O139 strain.…”

confidence: 99%