Induction of rat liver cytochrome P450 isoenzymes CYP 1A and CYP 2B by different fungicides, nitrofurans, and quercetin

Antibiotic Furazolidone Induces CYP1A But Not CYP2E1 Subfamily in Rat Liver

“…Then, we performed Western blot analysis, and clarified that the increase in reverent colonies in the Ames test using the FZtreated S-9 fraction was due to the induction of CYP1A1 apoprotein. Our present data on CYP1A1 are consistent with those of Rahden-Staron et al [19], showing that CYP1A was responsible for the enhanced mutagenicity in the Ames test of the S-9 fraction of rats treated intraperitoneally with FZ. We also demonstrated that the CYP1A1 isozyme was regulated transcriptionally, showing induction Sudan III-treated rat livers.…”

“…Cytochrome P450 (CYP) participates in the phase I metabolism of numerous drugs, and is reported to be especially affected by furazolidone treatment in rats and chickens [4,6,8,19,20]. In previous studies, multiple oral doses of FZ (25-200 mg/kg for 4 days) resulted in an increase in hepatic microsomal CYP concentrations in rats [20].…”

“…The authors did not determine the isoform of CYP induced, though the elevation of aniline hydroxylation suggests CYP2E1 induction. In addition, recently, administration of FZ (80 mg/kg by intraperitoneal injection for 3 days) showed induction or inducibility of the hepatic CYP1A subfamily in the Ames test using ethidium bromide (EtBr) as the mutagen [19]. Considering the above reports, it is speculated that repeated administration of FZ may result in the weak induction of hepatic CYP1A and/or CYP2E1 subfamily isoenzymes in rats.…”

Inhibitory effects of phthalimide derivatives on the activity of the hepatic cytochrome P450 monooxygenases CYP2C9 and CYP2C19

“…Later an impact of this fungicide on the activity of various CYPs was confirmed in mouse liver microsomes [24]. Incubation with captafol caused the same effects on different CYPs in the liver of rats [26,27]. Also indobufen was found to inhibit CYPs in experiments with rats, but only to toxicological tolerable extents [28].…”

International regulatory needs for development ofan IATA for non-genotoxic carcinogenic chemical substances

“…Induction of CYP 2B10 associated with CAR activation (Ren et al, 2010) In vitro liver microsomal CYP 1A2 induction (Lang et al, 1996(Lang et al, , 1997 Assessment of effects on CYP 1A1 and CYP 2B (Rahden-Staron et al, 2001) Level 0 (Q)SAR and structure activity information (Benigni, 2012;Serafimova et al, 2007; SAR and grouping based upon mammary gland tumor induction by various striazine compounds in rats (US EPA, 2002) Existing literature data (Rakitsky et al, 2000;NTP, 2008NTP, , 2011IARC, 1991 is important, also low probability of false negatives is critical.…”