Induction of rat liver tumor using the Sleeping Beauty transposon and electroporation

Park, June-Shine; Kim, Bae-Hwan; Park, Sung Goo; Lee, Do Hee; Son, Woo‐Chan

doi:10.1016/j.bbrc.2013.03.119

Cited by 6 publications

(6 citation statements)

References 17 publications

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“…When the mobile elements of the SB transposons that encode the oncogenes or silence tumor suppressor genes are expressed simultaneously within a cell, tumorigenesis can be induced. Many tumors, including liver [ 10 , 12 ], intestine [ 18 ], pancreas [ 19 ] and skin [ 20 , 21 ] tumors, have been generated using the SB transposon system in mice or rats. Although the SB system has been used to induce many types of tumors in mice, investigators have had difficulty generating prostate cancers with the SB system [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

“…Although various types of SB transposon-induced tumors have been reported, several types of tumors, such as tumors of the lung, mammary gland and prostate, have not yet been generated with the SB transposon system in the mouse model [ 5 , 12 ]. Low insertional mutagenesis efficiency would be one of the factors that are preventing a successful model of these tumors from being created.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Mouse Model of Prostate Cancer Using the Sleeping Beauty Transposon and Electroporation

et al. 2018

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The Sleeping Beauty (SB) transposon system is non-viral and uses insertional mutagenesis, resulting in the permanent expression of transferred genes. Although the SB transposon is a useful method for establishing a mouse tumor model, there has been difficulty in using this method to generate tumors in the prostate. In the present study, electroporation was used to enhance the transfection efficiency of the SB transposon. To generate tumors, three constructs (a c-Myc expression cassette, a HRAS (HRas proto-oncogene, GTPase) expression cassette and a shRNA against p53) contained within the SB transposon plasmids were directly injected into the prostate. Electroporation was conducted on the injection site after the injection of the DNA plasmid. Following the tumorigenesis, the tumors were monitored by animal PET imaging and identified by gross observation. After this, the tumors were characterized by using histological and immunohistochemical techniques. The expression of the targeted genes was analyzed by Real-Time qRT-PCR. All mice subjected to the injection were found to have prostate tumors, which was supported by PSA immunohistochemistry. To our knowledge, this is the first demonstration of tumor induction in the mouse prostate using the electroporation-enhanced SB transposon system in combination with c-Myc, HRAS and p53. This model serves as a valuable resource for the future development of SB-induced mouse models of cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a Mouse Model of Prostate Cancer Using the Sleeping Beauty Transposon and Electroporation

et al. 2018

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“…On the other hand, it is possible to generate disease models by stably introducing genes that cause disorders. This has been directly done for various cancer models induced by SB‐mediated oncogene transposition including sarcomatoid carcinoma, [ 115,116 ] prostate cancer, [ 117 ] hepatocellular carcinoma, [ 118 ] peritoneal carcinomatosis, [ 119 ] glioma, [ 120 ] HPV16 + oral tumors [ 121 ] but also for non‐cancer‐related disorders such as spinocerebellar ataxia type‐1, [ 122 ] age‐related renal pathologies, [ 123 ] and nephronophthisis‐related ciliopathies. [ 124 ] Additionally, with increasing demand of gene therapies and a climbing number of successful transitions of preclinical research into clinical trials and approved medicinal products, SB's attractive features have further widened its implementation in the fields of basic research and preclinical studies ( Table 1 ).…”

Section: Preclinical and Basic Research Applications Using The Sleepimentioning

confidence: 99%

Latest Advances for the Sleeping Beauty Transposon System: 23 Years of Insomnia but Prettier than Ever

Amberger

Ivics

2020

BioEssays

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The Sleeping Beauty transposon system is a nonviral DNA transfer tool capable of efficiently mediating transposition-based, stable integration of DNA sequences of choice into eukaryotic genomes. Continuous refinements of the system, including the emergence of hyperactive transposase mutants and novel approaches in vectorology, greatly improve upon transposition efficiency rivaling viral-vector-based methods for stable gene insertion. Current developments, such as reversible transgenesis and proof-of-concept RNA-guided transposition, further expand on possible applications in the future. In addition, innate advantages such as lack of preferential integration into genes reduce insertional mutagenesis-related safety concerns while comparably low manufacturing costs enable widespread implementation. Accordingly, the system is recognized as a powerful and versatile tool for genetic engineering and is playing a central role in an ever-expanding number of gene and cell therapy clinical trials with the potential to become a key technology to meet the growing demand for advanced therapy medicinal products.

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“…64 With the help of electroporation method, SB transposon system can also deliver c-Myc, H-RAS, and shp53 oncogenes into rats to produce liver tumor. 65 In addition, the SB100X transposon system has been ever used for enzyme-catalyzed gene integration into the embryonic porcine genome. 66 Thus, SB transposon system can be applied to construct a variety of animal models for genetic etiological research of cancers.…”

Section: -55mentioning

confidence: 99%

Sleeping Beautytransposon system for genetic etiological research and gene therapy of cancers

Hou¹,

Deng³

et al. 2014

Cancer Biology & Therapy

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Induction of rat liver tumor using the Sleeping Beauty transposon and electroporation

Cited by 6 publications

References 17 publications

Development of a Mouse Model of Prostate Cancer Using the Sleeping Beauty Transposon and Electroporation

Development of a Mouse Model of Prostate Cancer Using the Sleeping Beauty Transposon and Electroporation

Latest Advances for the Sleeping Beauty Transposon System: 23 Years of Insomnia but Prettier than Ever

Sleeping Beautytransposon system for genetic etiological research and gene therapy of cancers

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