Induction of resistance to antibody-mediated cytotoxicity. H-2, Ia, and Ig antigens are independent entities in the membrane of mouse lymphocytes.

Hauptfeld,; Hauptfeld, Miroslav; Klein, Jan

doi:10.1084/jem.141.5.1047

Cited by 43 publications

(19 citation statements)

References 17 publications

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“…The concordant reactions of several typing sera of high quality is strong evidence that the HLA-A, B, and C antigens were not affected by the "stripping" procedure. Although it is not known whetfier resistance to cytolysis in such experiments is actually due to removal (stripping) of the molecules as in antigen redistribution observed by immunofluorescence, it is well established that the method has a high degree ofspecificity (27,28). Thus, E antigens appear to be distinct and most likely located in separate molecules on the surface of E cells.…”

Section: Introductionmentioning

confidence: 99%

“…Antigen redistribution tests by the resistance-induction method. The procedure to test for the molecular independence of antigens on the surface of E cells was based on development of specific resistance to antibody-mediated lysis as described by Bemoco and co-workers (27) and more recently by Hauptfeld and co-workers (28 Antigen redistribution experiments were performed by the resistance-induction method (27,28) to investigate whether E antigens are separate from the products of the HLA-A, B, and C loci. In these experiments, resistance to lysis developed after treatment with specific anti-E antibodies, but the treated E cells remained susceptible to the cytotoxic effects of anti-HLA-A, B, and C reagents.…”

Section: Introductionmentioning

confidence: 99%

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A New Antigen System Expressed in Human Endothelial Cells

Moraes¹,

Šťastný²

1977

J. Clin. Invest.

125

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A B S T R A C T Kidney transplant recipients were previously found to have antibodies that reacted with cells isolated from the endothelium of umbilical cord veins and which were not cytotoxic for lymphocytes from the same donors. Results of the present experiments indicate that endothelial (E) antigens are different from previously known HLA antigens and also from Ia-like antigens of bone marrow-derived (B) lymphocytes. Attempts to absorb E antibodies with lymphocytes from E-positive donors failed in most cases. Antigen redistribution experiments shoved that E antigens were located in separate molecules from the products of HLA-A, B, and C. Thus, E cells treated with E antibody became resistant to lysis by the antibody used, but remained susceptible to the effects of typing sera for alleles of HLA-A, B, and C. Antibodies to E cells were also cytotoxic for blood monocytes. Moreover, monocytes were able to absorb E-antibody reactions, indicating that similar antigens were expressed in both cells. E antibodies did not react with B lymphocytes isolated from peripheral blood. In that regard E antibodies were different from antibodies to human Ialike antigens which reacted with E cells, monocytes, and isolated B lymphocytes. Thus it appears that E antigens constitute a system of human alloantigens which has not been previously identified. The possibility that these antigens play a role in kidney allograft rejection should now be investigated since matching can be performed using monocytes isolated from the blood of recipients and donors.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A New Antigen System Expressed in Human Endothelial Cells

Moraes¹,

Šťastný²

1977

J. Clin. Invest.

125

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“…Serological analysis of these antigens indicates that a product of each H-2K or H-2D allele is characterized by one private antigen, i.e., antigen specific for the particular allele (6), and an array of public antigens, i.e., antigens shared by products of several alleles (7). It should be emphasized that all antigens coded for by a particular allele are apparently carried by the same molecule or molecular complex (8). BiochemiciAl studies of the H-2K and H-2D antigens have revealed that thb antigens are glycoproteins with a molecular weight of 44,000 (9,10) which are attached noncovalently to ,B2-microglobulin (11)(12)(13)(14)(15), a 12,000-molecularweight protein devoid of carbohydrate (15).…”

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confidence: 99%

The partial amino-acid sequence of an H-2K molecule.

Vitetta¹,

Capra²,

Klapper³

et al. 1976

Proc. Natl. Acad. Sci. U.S.A.

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Eighteen of the NH2-terminal 27 amino acids of a murine H-2K molecule have been assigned. The approach used was to label murine splenocytes with a single radioactive amino acid, isolate the H-2K molecule by specific immunoprecipitation, electrophorese the dissolved precipitate on sodium dodecyl sulfate polyacrylamide gels, and subject the isolated H-2K peak to amino-acid analysis and automated sequencing.

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“…In the absence of reliable biochemical data, it could provide the missing link between serology and genetics in the study of a complex antigenic system . Summary Molecular relationship of public H-2 antigens 1, 5,6,8,11,13,25, and 28 to private antigens controlled by K and D regions was studied using the technique of antibody-induced resistance to complement-mediated cytotoxicity . The results indicate physical association in the cell membrane between H-2 antigens 1…”

Section: Discussionmentioning

confidence: 99%

“…Antibody-Mediated Induction of Resistance to Cytotoxicity (AMIRC) Technique. The method of AMIRC was described previously (11) . Briefly, spleen cells were suspended in Hanks' BSS and divided into aliquots, each containing 5 x 108 cells.…”

Section: Methodsmentioning

confidence: 99%

Molecular relationship between private and public H-2 antigens as determined by antigen redistribution method.

Hauptfeld¹,

Klein²

1975

The Journal of Experimental Medicine

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Classical H-2 antigens can be divided into two, not very sharply defined groups, private and public . Private H-2 antigens (1) have a restricted strain distribution : among inbred strains they are found in only one haplotype of independent origin ; public H-2 antigens (2), on the other hand, are shared by at least two, and quite often by a large number of unrelated haplotypes . Private H-2 antigens are believed to be controlled by alleles of two loci at the opposite ends of the H-2 complex, the H-2K locus in the K region, and the H-2D locus in the D region . In agreement with this belief, the antigens can be arranged in two series, where members of each series are mutually exclusive (3) . No such arrangement is possible for the public H-2 antigens and the nature of the genetic control of these antigens has long been a matter of controversy .According to the traditional interpretation of the H-2 complex (4), the public antigens are controlled by separate regions located between the K and D regions within the complex . According to the two-locus model (5), public H-2 antigens reside in the same molecules as the private ones and are controlled either by the H-2K or H-2D loci, or both . The two-locus model fits better the available data than the traditional multi-locus model (6) . Also, the former model is supported by the results of transplantation analysis demonstrating that only the peripheral regions of the H-2 complex are involved in rapid skin-graft rejection (7) . However, a formal proof that the serologically detectable public H-2 antigens are coded for by the K and D regions has never been provided . As a matter of fact, most recent developments (8, 9, cf. Discussion) suggest that an across the board assignment of public antigens to K and D regions could not be justified . Despite these developments, many investigators accept the two-locus model as an established fact and rely on it in their interpretation of the H-2 system . At this stage it is, therefore, critical to test whether the serologically detectable public H-2 antigens conform to the predictions of the two-locus model .We have recently developed a method that can be used to examine molecular relationships among various cell membrane antigens . The method is based on the observation that under certain circumstances, antigens coated with alloanti-* This investigation was supported by grant

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Induction of resistance to antibody-mediated cytotoxicity. H-2, Ia, and Ig antigens are independent entities in the membrane of mouse lymphocytes.

Cited by 43 publications

References 17 publications

A New Antigen System Expressed in Human Endothelial Cells

A New Antigen System Expressed in Human Endothelial Cells

The partial amino-acid sequence of an H-2K molecule.

Molecular relationship between private and public H-2 antigens as determined by antigen redistribution method.

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