Induction of respiratory deficiency by repression of the respiratory system in a mutant of Saccharomyces cerevisiae

Negrotti, Teresa; Wilkie, D.

doi:10.1016/0005-2728(68)90079-0

Cited by 19 publications

(2 citation statements)

References 23 publications

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“…Labeled precursors such as amino acids are taken up rapidly and their rate of incorporation into various products can be followed expediently. (3) It permits the use of well-defined nuclear and cytoplasmic genotypes for the performance of genetic crosses and the determination of the influence of genetic conditions on mitochondriogenesis (Sherman and Slonimski, 1964;Mackler et al, 1965;Negrotti and Wilkie, 1968;Thomas and Wilkie, 1968a,b;. (4) Its growth, and the development of its mitochondria, are subject to physiological regulation; for instance, when yeast is grown on 1 % glucose, a period of time can be elected where aerobic glycolysis is replaced by terminal oxidation of ethanol (and acetate) as a carbon and energy source.…”

mentioning

confidence: 99%

Formation of yeast mitochondria. I. Kinetics of amino acid incorporation during derepression

Henson¹,

Weber²,

Mahler³

1968

Biochemistry

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mentioning

confidence: 99%

Formation of yeast mitochondria. I. Kinetics of amino acid incorporation during derepression

Henson¹,

Weber²,

Mahler³

1968

Biochemistry

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“…This could result in a structural change of the inner mitochondrial membrane that may also alter enzymes and/or the attachment site of mitochondrial DNA . It has been suggested that petite mutants of yeast can be obtained efficiently as a result of a hereditarily or transiently induced membrane abnormality (46,47) . A membrane change in the F cell is suggested by our preliminary experiments in which the rate of L-FIGURE 11 Mitochondrial DNA of EB-resistant F2, F4, F8, and F16 cells, maintained in the presence of 2, 4, 8, and 16 µg EB/ml, respectively.…”

Section: (B)mentioning

confidence: 99%

Establishment and Characterization of Ethidium Bromide Resistance in Simian Virus 40-Transformed Hamster Cells

Klietmann

Sato

Nass

1973

The Journal of Cell Biology

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This study describes the isolation and subsequent characterization of four mammalian cell lines resistant to ethidium bromide (EB) . Treatment of the simian virus 40-(SV40) transformed hamster cell line F5-1 first led to the establishment of the F2 cell line, which is resistant to 2 ug EB/ml . At this concentration cytochromes c and b are present in almost normal or only slightly diminished amounts, whereas cytochromes a + as show an obvious decrease . The mitochondria of the F2 cell show a normal ultrastructure, not distinct from the parental cell line F5-1, and contain closed circular DNA . The sensitive parental F5-1 cells, however, when exposed to the same dye concentration exhibit the typical EB-induced ultrastructural changes in the mitochondria, and no more component I mitochondrial DNA can be demonstrated . 1 yr after establishment we derived from the F2 cell three more cell lines, resistant against 4, 8, and 16,ug of EB/ml . These cell lines, termed F4, F8, and F16, respectively, also revealed relatively intact-appearing mitochondria, although distinguishable from F5-1 and F2 mitochondria by a more condensed or unorthodox cristae conformation . F4, F8, and F16 cell lines contained closed circular mitochondrial DNA in the same position as that of the parental F5-1 cells, when analyzed in an isopycnic CsCI-EB gradient . A small shoulder at the lower density side of the DNA I peaks was observed . The newly acquired drug resistance of the F cells is hereditarily transmitted to the progeny cells and retained even after a period of growth in EB-free medium .

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Der Atmungsdefekt bei Hefezellen: eine kritische Betrachtung seiner Ursachen II. Der „RD‐Zustand”︁ (petite‐Mutation) und verwandte Defekte

Kraepelin

1972

J of Basic Microbiology

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EinleitungI n der Zeit seit dem Erscheinen des ersten Teiles dieses Sammelreferates (KRAEPELIN 1967) hat der Atmungsdefekt, soweit er sich auf die sogenannte plasmatische ,,petite-Mutation" bezieht, noch keine definitive Kliirung gefunden. Obgleich es zu diesem Thema bereits mehrere zusammenfassende Darstellungen gibt, in denen die zur Zeit wesentlich erscheinenden Fakten diskutiert wurden, mag die in manchem abweichende Betrachtungsweise dieses Referat dennoch rechtfertigen. Insbesondere mag es dazu beitragen, bekannte aber scheinbar unkorrelierte Phiinomene einmal im Zusammenhang zu sehen. Drt es noch keineswegs erwiesen ist, daD der plasmatische Atmungsdefekt und seine Begleiterscheinungen auf einer Mutation genetischen Materials beruhen, sol1 hier der kausal weniger festgelegten Bezeichnung ,,RD-Zustand" (respiratory defective state) auch weiterhin der Vorzug gegeben werdena). 17'gen. n., the heterobasidiomycetous stage of several yeasts of the genus Candida. Antonie van Leeuwenhoek, 86,433-462. FIIL, A. and BRANTON, D., 1969. Changes in the plasma membrane of Escherichia coli during magnesium starvation.

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Induction of respiratory deficiency by repression of the respiratory system in a mutant of Saccharomyces cerevisiae

Cited by 19 publications

References 23 publications

Formation of yeast mitochondria. I. Kinetics of amino acid incorporation during derepression

Formation of yeast mitochondria. I. Kinetics of amino acid incorporation during derepression

Establishment and Characterization of Ethidium Bromide Resistance in Simian Virus 40-Transformed Hamster Cells

Der Atmungsdefekt bei Hefezellen: eine kritische Betrachtung seiner Ursachen II. Der „RD‐Zustand”︁ (petite‐Mutation) und verwandte Defekte

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