Induction of rosmarinic acid accumulation in cell suspension cultures of Orthosiphon aristatus after treatment with yeast extract

Sumaryono, Wahono; Proksch, Peter; Hartmann, Thomas; Nimtz, Manfred; Wray, Victor

doi:10.1016/0031-9422(91)83190-v

Cited by 84 publications

(37 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since the first report that RA production could be enhanced by the addition of YE to the cell cultures of Orthosiphon arisatus (Sumaryono et al 1991), biochemical and molecular biological characterization of elicitation of RA biosynthesis has been extensively investigated using L. erythrorhizon (Boraginaceae) and Coleus blumei (Lamiaceae) cell cultures. We have shown that PAL in the phenylpropanoid pathway and HPR in the tyrosine-derived pathway were responsible for the elicitor-enhanced RA production in L. erythrorhizon cells (Mizukami et al 1993).…”

mentioning

confidence: 99%

Elicitor-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures: Activities of rosmarinic acid synthase and the final two cytochrome P450-catalyzed hydroxylations

Ogata

Tsuruga

Matsuno

et al. 2004

Plant Biotechnology

View full text Add to dashboard Cite

A drastic increase in rosmarinic acid (RA) accumulation is induced in cultured cells of Lithospermum erythrorhizon after their exposure to yeast extract or methyl jasmonate. Addition of the elicitors to the cell cultures only slightly enhanced the activity of rosmarinic acid synthase, which catalyzes formation of 4-coumaroyl-4Ј-hydroxyphenyllactic acid (CHPL), the first reaction specific to RA biosynthesis. In contrast, the two-step cytochrome P450-catalyzed hydroxylations of CHPL to form RA were dramatically up-regulated by the elicitor treatments, indicating that these hydroxylation activities are likely to play a key regulatory role in elicitation of RA biosynthesis.

show abstract

mentioning

confidence: 99%

Elicitor-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures: Activities of rosmarinic acid synthase and the final two cytochrome P450-catalyzed hydroxylations

Ogata

Tsuruga

Matsuno

et al. 2004

Plant Biotechnology

View full text Add to dashboard Cite

show abstract

“…The registered amounts of p-coumaric and cinapic acids were insignificant. To our knowledge, rosmarinic acid was produced in suspension culture of species of the Laminaceae (2,5,6,7,9,11), but this is the first report on cell culture from Lavandula vera, producing rosmarinic acid .…”

Section: - ------------- -120mentioning

confidence: 91%

“…Cultivation was performed in Erlenmeyer flasks with 1/5 net volume on the shaker (11,6 rad.sec-1 ) for 12 days. For inoculation 20 % (V IV) of the respective cell suspension was used, cultivated under the described conditions for 7 days.…”

Section: Methodsmentioning

confidence: 99%

“…1, during the growth cycle the cells secreted into the medium about 50-80 mg.dm-3 phenolic compounds, but their possible excretion in greater amounts can therefore not be excluded. The observed low level of extracellular phenolics (and particularly of the blue pigment) was presumably due to their continuous degradation by the enzymes of culture medium (11,12).…”

Section: - ------------- -120mentioning

confidence: 97%

See 1 more Smart Citation

Growth and Phenolics Production of Cell Suspension Culture ofLavandula Vera MM

Илиева¹,

Pavlov²,

Kovatcheva³

et al. 1995

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

The time course of growth and changes of phenolics formation were examined in Lavandula vera-lvflv! suspension culture. After 9 days of cultivation, 11 g.dm-3 of dry biomass was accumulated, which contained a sufficient amount of phenolic compounds (230 mg.dm-3 ). The HPLC analysis identified rosmarinic and caffeic acids as main components. To our knowledge this is the first report on cell culture from Lavandula vera producing rosmarinic acid.

show abstract

“…As plant cell cultures of a variety of pharmaceutical and essential oil plants produced disappointing yields when compared to the intact tissue (Charlwood, 1993) elicitation was propagated as a novel strategy to overcome the obvious metabolic restrictions. Examples were the increased production of rosmarinic acid (Sumaryono et al, 1991 ), of thiopene (Buitelaar et al, 1992), or of essential oils (Fauconnier et al, 1993). An elicitor from Botrytis was successfully applied on an industrial scale for the production of the antiflammatory compound sanguinarin by Papaver somniferum cells (Park etal., 1990).…”

Section: Introductionmentioning

confidence: 99%

Elicitation of volatile compounds in photomixotrophic cell culture of Petroselinum crispum

Reil

Berger

1996

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Photomixotrophic cells of Petroselinum crispum accumulated > 500 mg chlorophyll per kg wet weight and grew well in a broad range of phytoeffector conditions. Autoclaved fungal cells were lethal for photoheterotrophic cells, but induced in photomixotrophic cells the formation of volatile n-alkanes, phthalides, coumarins, and elemicine. Most of the compounds elicited reached a concentration maximum between 20 and 30 h after addition of the mycelium, whereas the group of n-alkanes increased steadily during the 90 h monitored. Maximum concentrations were: 12 mg of graveolone, 1 mg of bergapten, 0.5 mg of sedanenolide, and 0.5 mg of n-tetradecane per 1 nutrient medium. A dose/effect relationship was found; 10 to 25 g of fungal wet weight per 1 culture medium resulted in maximum accumulation of volatiles. The formation of volatiles by photomixotrophic in vitro cells is discussed as an integral part of plant responses to ecological stress.

show abstract

Induction of rosmarinic acid accumulation in cell suspension cultures of Orthosiphon aristatus after treatment with yeast extract

Cited by 84 publications

References 17 publications

Elicitor-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures: Activities of rosmarinic acid synthase and the final two cytochrome P450-catalyzed hydroxylations

Elicitor-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures: Activities of rosmarinic acid synthase and the final two cytochrome P450-catalyzed hydroxylations

Growth and Phenolics Production of Cell Suspension Culture ofLavandula Vera MM

Elicitation of volatile compounds in photomixotrophic cell culture of Petroselinum crispum

Contact Info

Product

Resources

About