Induction of secondary carotenogenesis in new halophile microalgae from the genus Dunaliella (Chlorophyceae)

Solovchenko, Alexei; Selivanova, E. A.; Chekanov, Konstantin; Sidorov, Roman A.; Немцева, Н. В.; Лобакова, Е. С.

doi:10.1134/s0006297915110139

Cited by 22 publications

(13 citation statements)

References 22 publications

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“…At the first "green" phase favourable conditions are created for high growth rate maintenance, thereby, biomass accumulation occurs. Then, at the second "red" phase, carotenogenesis is induced in D. salina cells by different stress factors, such as salinity and illumination increase, mineral nutrient limiting (Solovchenko et al, 2015;Lv et al, 2016;Minhas, Hodgson, Barrow, & Adholeya, 2016;Hamed, Ak, Isık, & Uslu, 2017;Achour, Doumandji, Bouras, Sabaou, & Assunҫão, 2019).…”

Section: Introductionmentioning

confidence: 99%

Production Characteristics of Dunaliella salina at Two-phase Pilot Cultivation (Crimea)

Боровков¹,

Gudvilovich²,

Avsiyan³

et al. 2020

Turk. J. Fish. Aquat. Sci.

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Productional and biochemical characteristics of green alga Dunaliella salina (Dunal) Teodoresco were studied during pilot cultivation at the southwest of Crimean Peninsula, Russia. Two-phase cultivation was carried out in ponds located in the greenhouse module. In the "green" phase, biomass concentration reached 1.2 g/l, with maximum cell density of 1.69•10 6 cell/ml and the maximum productivity of 0.08 g/(l•day) (7 g/(m 2 •day)). Twofold culture dilution was applied for carotenogenesis induction. The maximum cell density in the "red" phase was 0.84•10 6 cell/ml, while biomass reached 0.95 g/l. Mean net carotenoid accumulation rate at the second stage (1-6 day) was 0.65 mg/(l•day). Carotenoids content in culture doubled compared to initial values and reached about 20 mg/l or 800 mg/m 2. Carotenoids/chlorophyll a ratio of 11.6 was observed by the end of the "red" phase. Night biomass loss was higher in the "red" phase (up to 9.5% of biomass) compared to the "green" phase (up to 5.6%). Optimal duration of both the first and the second cultivation stages was found to be 10-12 days for the studied weather conditions, which enables production of 3 g of carotenoids from 1 m 2 (100 l) of starter culture. In conclusion, Crimea is a prospective region for D. salina algobiotechnology.

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Section: Introductionmentioning

confidence: 99%

Production Characteristics of Dunaliella salina at Two-phase Pilot Cultivation (Crimea)

Боровков¹,

Gudvilovich²,

Avsiyan³

et al. 2020

Turk. J. Fish. Aquat. Sci.

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“…RCC802, for example, does not grow below salinities of 15 g·L −1 NaCl and accumulates high amounts of carotenoids under this low-salinity condition. The induction of carotenogenesis by salinity has been documented in other microalgae such as Haemotococcus or Dunaliella [ 33 , 34 ]. Carotenoid accumulation, in contrast, is enhanced under high temperature in OTTH595, a strain that was isolated from the Thau Lagoon, an environment marked by large temperature fluctuations ( Table 3 and Table S3 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Culture Conditions for the Optimization of Carotenoid Production in Several Strains of the Picoeukaryote Ostreococcus

et al. 2018

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Microalgae are promising sources for the sustainable production of compounds of interest for biotechnologies. Compared to higher plants, microalgae have a faster growth rate and can be grown in industrial photobioreactors. The microalgae biomass contains specific metabolites of high added value for biotechnology such as lipids, polysaccharides or carotenoid pigments. Studying carotenogenesis is important for deciphering the mechanisms of adaptation to stress tolerance as well as for biotechnological production. In recent years, the picoeukaryote Ostreococcus tauri has emerged as a model organism thanks to the development of powerful genetic tools. Several strains of Ostreococcus isolated from different environments have been characterized with respect to light response or iron requirement. We have compared the carotenoid contents and growth rates of strains of Ostreococcus (OTTH595, RCC802 and RCC809) under a wide range of light, salinity and temperature conditions. Carotenoid profiles and productivities varied in a strain-specific and stress-dependent manner. Our results also illustrate that phylogenetically related microalgal strains originating from different ecological niches present specific interests for the production of specific molecules under controlled culture conditions.

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“…Рассмотрим процесс синтеза вторичных каротиноидов при лимитирования роста D. salina биогенными элементами питательной среды и избытке солнечной энергии. В таких условиях фотобиосинтез клеток направлен в сторону увеличения содержания резервных липидов, углеводов, а также каротиноидов [18,19]. На рисунке 2 представлены данные по динамике содержания хлорофиллов, суммарных каротиноидов и плотности культуры.…”

Section: модель 3 накопление вторичных каротиноидовunclassified

Modeling the Dynamics of Pigment Content in Cells of Dunaliella Salina Teod. Unicellular Alga at the Stage of Carotenogenesis

Lelekov¹,

Боровков²,

Новикова³

et al. 2019

Math.Biol.Bioinf.

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The work is focused on modeling of chlorophyll and carotenoids content dynamics in the in cells of the unicellular algae D. salina, living in salt water, at carotenogenesis induction phase. A mathematical model of pigments content in microalgae cells, which experience excess of light energy and the limit of nutrient medium mineral components, is proposed. The model is based on assumption, that observed rate of variation in pigment concentration is an algebraic sum of the rates of synthesis, photodestruction and partial recovery of photo-oxidized pigments. The rate of secondary carotenoids synthesis does not depend on external conditions and is determined by the quantity of key enzyme complex and its turnover rate. The rate of secondary carotenoids and chlorophyll photodestruction depends on the effective light intensity and is proportional to the amount of absorbed photosynthetically active radiation energy. The verification of the derived equations was conducted in the course of D. salina cultivation at the carotenogenesis stage. The specific rate of chlorophyll a photodestruction was determined, which resulted in 0.12 days–1. The secondary carotenoids concentration increases up to the maximum value, which is determined by the ratio of synthesis and photodestruction specific rates, as well as the maximum culture density. Under conditions of natural light in the Sevastopol region, the maximum concentration of carotenoids was 18.33 mg/l or 0.73 g/m2.

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Induction of secondary carotenogenesis in new halophile microalgae from the genus Dunaliella (Chlorophyceae)

Cited by 22 publications

References 22 publications

Production Characteristics of Dunaliella salina at Two-phase Pilot Cultivation (Crimea)

Production Characteristics of Dunaliella salina at Two-phase Pilot Cultivation (Crimea)

Comparative Analysis of Culture Conditions for the Optimization of Carotenoid Production in Several Strains of the Picoeukaryote Ostreococcus

Modeling the Dynamics of Pigment Content in Cells of Dunaliella Salina Teod. Unicellular Alga at the Stage of Carotenogenesis

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