Induction of secondary metabolite production in transformed callus of Ammi majus L. grown after electromagnetic treatment of the culture medium

Królicka, Aleksandra; Kartanowicz, Rafał; Wosiński, Stanisław; Szpitter, A.; Kamiński, Marian; Łojkowska, Ewa

doi:10.1016/j.enzmictec.2006.03.042

Cited by 27 publications

(7 citation statements)

References 32 publications

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“…In the present work, we evaluated Ammi majus L. fruits as a source of furanocoumarins, especially IsoP, which was isolated and quantitatively analyzed in extracts obtained using PLE/ASE. Since pressurized liquid extraction was previously shown to give the highest yield of simple coumarins and furanocoumarins from plants from the Apiaceae family [ 7 , 20 , 40 , 41 , 42 ], this eco-friendly technique (less time, less energy, and a solvent-consuming procedure) was used to find the optimal conditions for the extraction of target compounds. Based on conventional heating in an oven at elevated temperatures (normally exceeding the boiling point of the extracting solvent) and at pressures up to 200 bar, this dynamic extraction requires a very a short time and a small volume of the solvent and ensures complete extraction in short time with a small volume of an organic solvent due to the better penetration of the sample [ 7 , 20 , 40 , 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, the PLE/ASE/HPLC methodology for quantitative or preparative purposes has not been used for A. majus fruit matrix to date, which was confirmed by the search in multiple databases (SciFinder CAS, Science Direct, Web of Science Clarivate, Medline, Pub-Med) (years 1990–2024; search topics; “(Ammi majus) and ASE”; ‘(Ammi majus) and PLE”). There is only one report by Królicka et al on the use of the ASE method for the evaluation of the components in transformed Ammi majus callus cultures [ 20 ], where the content of two coumarins, umbelliferon and bergapten, was estimated with the ASE/HPLC method.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Analysis of Isopimpinellin from Ammi majus L. Fruits and Evaluation of Its Biological Effect on Selected Human Tumor Cells

Bartnik,

Sławińska-Brych,

Mizerska-Kowalska

et al. 2024

Molecules

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Ammi majus L. (Apiaceae) is a medicinal plant with a well-documented history in phytotherapy. The aim of the present work was to isolate isopimpinellin (5,8-methoxypsoralen; IsoP) from the fruit of this plant and evaluate its biological activity against selected tumor cell lines. The methanol extract obtained with the use of an accelerated solvent extraction (ASE) method was the most suitable for the quantitative analysis of coumarins in the A. majus fruit matrix. The coumarin content was estimated by RP-HPLC/DAD, and the amount of IsoP was found to be 404.14 mg/100 g dry wt., constituting 24.56% of the total coumarin fraction (1.65 g/100 g). This, along with the presence of xanthotoxin (368.04 mg/100 g, 22.36%) and bergapten (253.05 mg/100 g, 15.38%), confirmed A. majus fruits as an excellent source of these compounds. IsoP was isolated (99.8% purity) by combined liquid chromatography/centrifugal partition chromatography (LC/CPC) and tested for the first time on its antiproliferative activity against human colorectal adenocarcinoma (HT29, SW620), osteosarcoma (Saos-2, HOS), and multiple myeloma (RPMI8226, U266) cell lines. MTT assay results (96 h incubation) demonstrated a dose- and cell line-dependent decrease in cell proliferation/viability, with the strongest effect of IsoP against the Saos-2 cell line (IC50; 42.59 µM), medium effect against U266, HT-29, and RPMI8226 (IC50 = 84.14, 95.53, and 105.0 µM, respectively), and very weak activity against invasive HOS (IC50; 321.6 µM) and SW620 (IC50; 711.30 µM) cells, as well as normal human skin fibroblasts (HSFs), with IC50; 410.7 µM. The mechanistic study on the Saos-2 cell line showed that IsoP was able to reduce DNA synthesis and trigger apoptosis via caspase-3 activation. In general, IsoP was found to have more potency towards cancerous cells (except for HOS and SW620) than against healthy cells. The Selective Index (SI) was determined, underlining the higher selectivity of IsoP towards cancer cells compared to healthy cells (SI = 9.62 against Saos-2). All these results suggest that IsoP might be a promising molecule in the chemo-prevention and treatment of primary osteosarcoma.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Isopimpinellin from Ammi majus L. Fruits and Evaluation of Its Biological Effect on Selected Human Tumor Cells

Bartnik,

Sławińska-Brych,

Mizerska-Kowalska

et al. 2024

Molecules

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“…They suggested that protein accumulation may be related to the synthesis of stress tolerance proteins and alleviation of stress conditions. Krolicka et al [25] presume that ADR-4 through its influence on water in transformed callus culture of Ammi majus L. stimulated the plant's defence system that resulted in an increase of coumarin and furanocoumarin production.…”

Section: Discussionmentioning

confidence: 99%

Effects of Electromagnetic Fields and their Shielding on the Quality of Carrot (Daucus Carota L.) Seeds

Gorski

Rosińska

Szopińska

et al. 2019

Ecological Chemistry and Engineering S

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The aim of the study was to determine the effect of electromagnetic fields and their shielding on carrot seed quality. Three sectors were separated on the device emitting electromagnetic fields: “E” - sector emitting electromagnetic radiation with the predominance of the electrical component, “EM” - sector emitting electromagnetic radiation without domination of its components and “M” - sector with a predominance of magnetic component. Fields generated by the device were also shielded with ADR TEX screen, based on a nanocomposite in which the electric component of the electromagnetic radiation is absorbed by water dispersed in a dielectric matrix in various ways. The composites exhibit high dielectric absorption and shield electric fields within the frequency range from ~100 mHz to ~100 kHz. Seed germination and vigour were evaluated at 20 °C in darkness. Mycological analysis was performed using a deep-freeze blotter test. Exposure of seeds to radiation with the predominance of the electrical component and electromagnetic radiation without domination of its components combined with shielding of electromagnetic fields with ADR TEX (E+ADR TEX and EM+ADR TEX) increased seed germination energy and germination capacity compared to these treatments without shielding and control. The percentage of abnormal diseased seedlings in treatments with shielding of electromagnetic fields with ADR TEX (E+ADR TEX, EM+ADR TEX and M+ADR TEX) was significantly lower than in the treatments without shielding and in control. None of the treatments affected seed vigour. Generally, exposure of seeds to electromagnetic radiation did not influence the incidence of fungi.

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“…Aunque tiene potencial ornamental, la investigación actual se ha enfocado en sus propiedades medicinales, debidas que es fuente importante de cumarinas y furanocumarinas, substancias conocidas por su efecto curativo en diversos padecimientos de la piel en seres humanos y por su efecto fungicida y bactericida en plantas (Ojala et al, 2000;Królicka et al, 2006). La mayoría de los trabajos se han realizado en cultivos in vitro (Królicka et al, 2001;Deepshikha et al, 2002;Staniszewska et al, 2003) y algunos con partes aéreas de la planta (Hamerski et al, 1990;Nasser, 1998).…”

Section: Introductionunclassified

“…Although it has ornamental potential, current research has focused on its medicinal properties, due to a significant source of coumarins and furanocoumarins, known for its healing effect on various skin conditions in humans and their fungicidal and bactericidal effect on plants (Ojala et al, 2000;Królicka et al, 2006). Most studies were performed in vitro cultures (Królicka et al, 2001;Deepshikha et al, 2002;Staniszewska et al, 2003), and some aerial plant parts (Hamerski et al, 1990;Nasser, 1998).…”

Section: Introductionmentioning

confidence: 99%

Micronutrientes y rendimiento en Ammi majus como respuesta a la aplicación de composta

Ruiz-Bello

Trejo-Téllez

Pérez-Vázquez

2018

Remexca

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La especie Ammi majus tiene potencial ornamental pero el principal motivo de estudio es su contenido de cumarinas y furanocumarinas, substancias utilizadas como base en productos para padecimientos de la piel. El objetivo de este trabajo es conocer el rendimiento y absorción de micronutrientes con la aplicación de composta. El experimento se realizó en 2013 en Texcoco, México. Los tratamientos consistieron en mezcla suelo:composta 1:1 y suelo solo; a dos de las mezclas se les agregó 20 y 30% agrolita y a una tercera se le adicionaron 100 g de fertilizante N-P-K generando cinco tratamientos. Las plantas se cosecharon a los cinco meses de edad; se secaron a 71 °C, se pesaron y se molieron para el análisis de Fe, Cu, Mn y Zn. Se analizó pH, conductividad eléctrica, materia orgánica y capacidad de intercambio catiónico de los sustratos. La composta no tuvo efecto en la producción de materia seca, pero afectó significativamente el pH, la CE y la CIC de los sustratos. Las mezclas con agrolita y la mezcla con fertilizante, produjeron los rendimientos más altos; en estos, el pH subió y la CE bajó, ambos significativamente. Solo el Mn manifestó diferencias significativas, su absorción aumentó con la composta y con la disminución del pH inducida por el fertilizante. Se concluye que la adición de composta incrementó el rendimiento de Ammi majus, debido a los cambios inducidos en el sustrato y no necesariamente al aporte de nutrientes.

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Induction of secondary metabolite production in transformed callus of Ammi majus L. grown after electromagnetic treatment of the culture medium

Cited by 27 publications

References 32 publications

Quantitative Analysis of Isopimpinellin from Ammi majus L. Fruits and Evaluation of Its Biological Effect on Selected Human Tumor Cells

Quantitative Analysis of Isopimpinellin from Ammi majus L. Fruits and Evaluation of Its Biological Effect on Selected Human Tumor Cells

Effects of Electromagnetic Fields and their Shielding on the Quality of Carrot (Daucus Carota L.) Seeds

Micronutrientes y rendimiento en Ammi majus como respuesta a la aplicación de composta

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