Induction of sexual reproduction in Sclerotium rolfsii, Ustilago cynodontis, and Cintractia limitata by Cyperus rotundus rhizomes

Abstract

Cyperus rotundus rhizomes induced sexual states in three fungi belonging to different genera, viz. Sclerotium rolfsii, Ustilago cynodontis, and Cintractia limitata. Rhizome meal and methanolic extract induced the formation of the sexual state in S. rolfsii while aqueous extract of Cyperus rotundus was most effective in inducing sexual reproduction in U. cynodontis and Cintractia limitata. Keywords: Cyperus rotundus, fungi, rhizomes, sexual reproduction.

Antifungal efficacy of some ethyl acetate extract fractions ofCyperus rotundusrhizomes against spore germination of some fungi

“…Singh et al (1996) reported that the rhizome meal as well as methanolic extract of rhizomes of C. rotundus induced basidial stage (perfect stage) formation in Sclerotium rolfsii, Ustilago cynodontis and Cintractia limitata. Similarly, the ethyl acetate extract of C. rotundus rhizomes inhibited spore germination of Fusarium udum .…”

Induction of Sexual Stage and Colony Morphology of Some Isolates ofSclerotium rolfsiiCausing Spotted Leaf Rot in Plants

“…Induction of sexual stage in S. rolfsii is infrequent in nature (West, 1947) but has been induced in culture (Mundkur, 1934; Mishra and Haque, 1960; Punja, 1985; Singh et al, 1996). The extent of hymenial development was influenced by the medium on which isolates were grown, the conditions of inoculation, the isolate, diameter of mycelial plugs and light intensity (Punja et al, 1982; Prithiviraj et al, 2000).…”

“…Tu and Kimbrough (1978) described Athelia rolfsii (Curzi) Tu & Kimbrough as the perfect stage of S. rolfsii while studying Rhizoctonia-Sclerotium complex which is the currently accepted binomial for the sexual stage of S. rolfsii (Punja et al, 1982). Basidial stage of S. rolfsii has been reported as of infrequent occurrence in nature (West, 1947; Ghosh and George, 1955; Kulkarni and Ahmed, 1967) but can be induced in culture (Goto, 1930; Mundkur, 1934; Misra and Haque, 1960; Ahmed and Kulkarni, 1966; Kulkarni and Ahmed, 1967; Ahmed et al, 1966; Punja et al, 1982; Singh et al, 1996). Singh et al (1996) were able to induce sexual stage of this fungus within a week on Cyperus rotundu s rhizome powder supplemented water agar medium.…”

“…Basidial stage of S. rolfsii has been reported as of infrequent occurrence in nature (West, 1947; Ghosh and George, 1955; Kulkarni and Ahmed, 1967) but can be induced in culture (Goto, 1930; Mundkur, 1934; Misra and Haque, 1960; Ahmed and Kulkarni, 1966; Kulkarni and Ahmed, 1967; Ahmed et al, 1966; Punja et al, 1982; Singh et al, 1996). Singh et al (1996) were able to induce sexual stage of this fungus within a week on Cyperus rotundu s rhizome powder supplemented water agar medium. The athelial stage induction of S. rolfsii on C. rotundus rhizome meal agar (CRMA) medium has been confirmed by some other worker also (Singh, 2002).…”

Role of air and Light in Sclerotial Development and Basidiospore Formation in Sclerotium Rolfsii

“…However, in all these reports, the time required for basidiospore formation ranged from two weeks to a few months. Singh et al (1996) developed a very simple new medium (6% Cyperus rotundus rhizome powder, agar agar 20 g and 1 000 cm 3 distilled water) on which basidiospores were formed in 6-8 days. In the present experiment we report the effect of air and light on sclerotial development and basidiospore formation of S. rolfsii.…”