Induction of sister-chromatid exchanges by d(42 MeV)-Be neutrons in unstimulated human-blood lymphocytes

Abstract

The ability of d(42 Mev)-Be neutrons and 250 kV X rays to produce sister-chromatid exchanges (SCE) has been re-examined using unstimulated (G0) human-blood lymphocytes. Contrary to a previous report, the neutrons produced a significant and dose-dependent increase in SCE. X rays, as previously, produced no measurable increase at any absorbed dose. The relative biological effectiveness (RBE) for this end-point is therefore undefined and effectively infinite. In contrast to the findings of many workers, the between-cell distributions of SCE were, in most cases, underdispersed. This could imply that the SCE burden of a cell was not selectively neutral in these experiments.

Absence of genotoxicity in human blood cells exposed to 50 Hz magnetic fields as assessed by comet assay, chromosome aberration, micronucleus, and sister chromatid exchange analyses

“…As shown, no differences are evidenced among groups in the frequency of SCE. It is worthwhile to note that SCE are not induced by ionizing radiation exposure, as reported by other authors [Morgan and Crossen, 1980;Savage and Holloway, 1988]. A slight but statistically significant decrease is evidenced in PRI between sham control and ELF treated samples (P <.05) and between positive control and samples subjected to combined exposure (P <.01) (Fig.…”

Micronuclei and chromosome aberrations in subjects occupationally exposed to antineoplastic drugs: a multicentric approach

“…Similar to MN evaluation, CA slides were coded and the entire analysis was carried out blindly. An average 100 well-spread metaphases containing 46 (±1) centromeres were analyzed for each subject; CA were classified according to the International System of Cytogenetic Nomenclature (ISCN) (Savage and Holloway 1988). Total CA included chromosome-type (chromosome-type breaks, ring chromosomes, dicentrics) and chromatid-type (chromatid-type breaks and chromatid exchanges) aberrations (CsA and CtA, respectively).…”

Sister Chromatid Exchanges and Chromosome Aberration Frequencies in Plutonium Workers

“…1976) or those potentially induced by internal x-irradiation from 24 'Am contamination (Littlefield et al 1981) . In contrast, high-LET neutrons (Savage and Holloway 1988) or a-particles (Aghahamohammadi et al . 1988) were reported to cause significant increases in SCE in human G o lymphocytes exposed in vitro .…”