Induction of Smooth Muscle Cell Phenotype in Cultured Human Prostatic Stromal Cells

Poster Abstracts

“…Co-encapsulation of drugs in nanocarriers constitutes a promising approach for tumor drug delivery of fixed drug ratios aiming at achieving higher efficacy and reduced side effects [1,2]. Materials and methods: In vitro quantitative evaluation of combinations with conventional and innovative drugs, at different ratios and in free or nano-encapsulated form, was performed and cytotoxicity assessed by the resazurin assay [3]. Determination of synergism, additivity or antagonism, against the HRPC PC-3 cell line, was performed by the median effect analysis [4].…”

“…TGFß has been intensely studied, the factor is pleiotropic and its function defined by organ-and cell-type. In prostate, TGFß has been implicated in smooth muscle differentiation [3,4]. Prostates from one day old rats were removed and separated into individual lobes.…”

“…Tumor growth was measured weekly by transrectal ultrasonography, and plasma PSA concentrations were measured at regular intervals. Mice were sacrificed when the tumor volume exceeded 1000 mm 3 Single cells obtained from the androgensensitive human PCa xenograft PC346-P were first selected for their adherence to collagen I (a2-integrin+). Subsequently, CD133+ -cells were isolated by magnetic beads associated cell sorting (MACS).…”

Transforming growth factor-beta induces growth inhibition and IGF-binding protein-3 production in prostatic stromal cells: abnormalities in cells cultured from benign prostatic hyperplasia tissues

“…Prostatic tissues were obtained from radical prostatectomy or open prostatectomy specimens and processed for cell culture as previously described (Peehl 1992, Peehl & Sellers 1997. The remainder of the prostate was fixed and serially sectioned.…”

“…Non-specific binding was blocked with 10% horse serum, then the cells were incubated with a polyclonal rabbit antiserum against IGFBP-3 (1:400) (Diagnostic Systems). Binding of the primary antibody was detected with biotinylated antirabbit IgG, the avidin-biotin complex reagent, and the substrate diaminobenzidine as previously described (Peehl & Sellers 1997).…”