Induction of somatic embryogenesis in Pinus roxburghii Sarg.

Arya, Sarita; Kalia, Rajwant K.; Arya, I. D.

doi:10.1007/s002990000197

Cited by 39 publications

(20 citation statements)

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“…is a wind-pollinated, monoecious subtropical pine species. It is found in a fairly continuous distribution throughout the monsoon belt between 72 • E and 95 • E longitude and 27 • N and 35 • N latitude; and is abundant on the hill slopes of both the western and eastern Himalayas between altitudes of 450 m to 2 300 m above sea level (Arya et al, 2000). Naturally, it is distributed from Bhutan (only in drier areas), Northern India (Jammu and Kashmir, Punjab, Himanchal Pradesh, Uttarakhand, Sikkim), Nepal, south of Tibet and Pakistan to Afghanistan (Dogra, 1985;Yi and Raven, 1999).…”

Section: Introductionmentioning

confidence: 99%

Genetic similarity of natural populations and plantations of Pinus roxburghii Sarg. in Nepal

et al. 2009

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Abstract• Genetic structures of five population pairs each consisting of one natural population and one neighbouring plantation of Pinus roxburghii Sarg. in Nepal were assessed using nuclear (nSSRs) and chloroplast microsatellite loci (cpSSRs).• The mean number of alleles at nSSRs loci in natural populations was 5.0 compared to 4.93 in plantations while the average observed heterozygosities were the same in both groups (H 0 = 0.50). Most of the alleles were common to all the populations, indicating that the populations correspond to a single genetic entity. Similarly forty-seven haplotypes were observed in natural populations compared to fifty haplotypes in plantations. Mean haplotype diversities of natural populations (0.953) and plantations (0.955) were very similar. Genetic diversity of Pinus roxburghii was relatively high with low or no evidence of inbreeding while genetic differentiation among all populations was very low (about 1%).• The very low differentiation among natural populations indicates efficient long-distance gene flow among populations resulting in homogeneous genetic structures at least at selectively neutral loci. Even though the harvest and production of seeds and seedlings was largely uncontrolled, genetic structures of most plantations show no signs of reduced variation, inbreeding or other negative effects compromising the adaptedness or adaptability of planted forests. • Les structures génétique de cinq paires de populations composées chacune d'une population naturelle et d'une plantation voisine de Pinus roxburghii Sarg. au Népal ont été évalués à l'aide de loci nucléaires (nSSRs) et de loci microsatellites chloroplastiques (cpSSRs). Mots• Le nombre moyen d'allèles à loci nSSRs dans les populations naturelles était de 5,0 comparé à 4,93 dans les plantations alors que la moyenne des hétérozygoties observées étaient les mêmes dans les deux groupes (H 0 = 0,50). La plupart des allèles étaient communs à toutes les populations, indiquant que les populations correspondent à une seule entité génétique. De même, quarante-sept haplotypes ont été observés dans les populations naturelles par rapport à cinquante haplotypes dans les plantations. La diversité moyenne des haplotypes des populations naturelles (0,953) et des plantations (0,955) étaient très similaires. La diversité génétique de Pinus roxburghii a été relativement importante, avec peu ou pas de preuve de consanguinité tandis que la différenciation génétique entre les populations était très faible (environ 1 %).• Le très faible différenciation entre les populations naturelles indique des flux de gènes efficaces à longue distance entre les populations issues de structures génétiques homogènes avec au moins une sélectivité neutre des loci. Même si la récolte et la production de semences et de plants ont été largement incontrôlées, les structures génétiques de la plupart des plantations ne montrent aucun signe de réduction de la variation de la consanguinité ou d'autres effets négatifs compromettant la faculté d'adaptation des forêts plant...

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Section: Introductionmentioning

confidence: 99%

Genetic similarity of natural populations and plantations of Pinus roxburghii Sarg. in Nepal

et al. 2009

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“…Este resultado es consistente con el reportado por Rebolledo et al (2006), quienes ensayaron los mismos agentes desinfestantes y tiempos de inmersión pero en semillas de Pinus jaliscana y Pinus pseudostrobus. Para otras especies de Pinus también se reporta el uso de otros agentes desinfectantes con los que se obtienen resultados satisfactorios como es el cloruro de mercurio (Tamta y Palmi 2004;Oliveira et al, 2011) y el peróxido de hidrógeno (Arya et al, 2000;Zacarias et al, 2006;Robledo et al, 2009). …”

Section: Resultados Y Discusión Desinfección Y Establecimiento In Vitrounclassified

“…Particularmente, para la familia Pinaceae existen antecedentes del empleo de estas técnicas para la propagación de algunas especies como: Pinus maximartinezii (Zacarias et al, 2006;Paz et al, 2009), Pinus pseudostrobus y Pinus jaliscana (Rebolledo et al, 2006); Pinus roxburghii (Arya et al, 2000); Picea chihuahuana (López et al, 2000), pero solo se documenta la propagación in vitro de Cedrus deodara (Rebolledo et al, 2006).…”

Section: Introductionunclassified

“…This result is consistent with that reported by Rebolledo et al (2006), who tested the same disinfectant agents and immersion times but in seeds of Pinus jaliscana and Pinus pseudostrobus. For other pinus species also reported the use of other disinfecting agents with which obtained satisfactory results such as mercuric chloride (Tamta and Palmi 2004;Oliveira et al, 2011) and hydrogen peroxide (Arya et al, 2000;Zacarias et al, 2006;Robledo et al, 2009). contaminados entre el total de explantes sembrados.…”

Section: Disinfection and In Vitro Establishmentmentioning

confidence: 99%

“…Particularly for the Pinaceae family there is a history of the use of these techniques for the propagation of some species such as Pinus maximartinezii (Zacarias et al, 2006Paz et al, 2009, Pinus pseudostrobus and Pinus jaliscana (Rebolledo et al, 2006); Pinus roxburghii (Arya et al, 2000); Picea chihuahuana (López et al, 2000); but only in vitro propagation of Cedrus deodara is documented (Rebolledo et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Estudio preliminar para la propagación in vitro de Cedrus atlantica mediante yemas axilares

Montes-Salazar

Sepúlveda-Jiménez

Evangelista‐Lozano

et al. 2017

Remexca

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ResumenCedrus atlantica es una planta ornamental utilizada para la decoración de parques y jardines. Con el fin de micropropagar material de calidad de esta planta, se implementó un protocolo de multiplicación in vitro. Para ello, como explantes se usaron las yemas axilares que se desinfestaron con hipoclorito de sodio al 10% por 15 min, tratamiento con lo que se obtuvo un 83% de material aséptico. El cultivo de los explantes se realizó en el medio basal McCown's Woody Plant (WPM) adicionado con carbón activado (1 g L -1) y cisteína (25 mg L -1 ), lo que permitió obtener cultivos sin rasgos de oxidación. En la etapa de multiplicación con el uso de la bencil amino purina (5 mg L -1 ) se obtuvieron hasta 5 brotes por explante, que presentaron un crecimiento de 1.4 cm. Sin embargo, el ácido indol butírico a 2.5 y 5 mg L -1 no indujo la formación de raíces en los brotes in vitro.Palabras clave: cedro, brotación, micropropagación, plantas leñosas. IntroducciónCedrus atlantica es una especie arbórea perteneciente a la familia Pinacea conocido como el verdadero cedro. La planta se introdujo a México como una planta ornamental. AbstractCedrus atlantica is an ornamental plant used to decorate parks and gardens. In order to micropropagate quality material of this plant, an in vitro multiplication protocol was implemented. For this, the axillary buds were used as explants which were disinfected with sodium hypochlorite 10% for 15 min, treatment with which obtained 83% aseptic material. Explants culture was performed in the basal medium McCown's Woody Plant (WPM) added with activated carbon (1g L -1 ) and cysteine (25 mg L -1 ), which allowed to obtain crops without oxidation features. In the multiplication stage with the use of benzyl amino purine (5 mg L -1 ) obtained up to 5 shoots per explant, which showed an increase of 1.4 cm. However, indole butyric acid at 2.5 and 5 mg L -1 did not induce root formation in vitro shoots.

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Somatic Embryogenesis in Pinus nigra Arn.: Some Physiological, Structural and Molecular Aspects

Salaj

Moravčíková

Salaj

Plant Cell Monographs

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Induction of somatic embryogenesis in Pinus roxburghii Sarg.

Cited by 39 publications

References 0 publications

Genetic similarity of natural populations and plantations of Pinus roxburghii Sarg. in Nepal

Genetic similarity of natural populations and plantations of Pinus roxburghii Sarg. in Nepal

Estudio preliminar para la propagación in vitro de Cedrus atlantica mediante yemas axilares

Somatic Embryogenesis in Pinus nigra Arn.: Some Physiological, Structural and Molecular Aspects

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