Induction of specific cell responses to a Ca3SiO5-based posterior restorative material

Laurent, Patrick; Camps, Jean; Méo, Michel De; Déjou, Jacques; About, Imad

doi:10.1016/j.dental.2008.02.020

Cited by 266 publications

(232 citation statements)

References 49 publications

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“…The purpose of our study was to simulate the use of the product by following the manufacturer's recommendations for clinical application in human teeth, which indicated using the undiluted product and a specific recommended dose. The higher frequency of micronuclei in our study compared with the results reported by Laurent et al 5 may have occurred because we did not dilute the Ca 3 SiO 5 .…”

Section: Discussioncontrasting

confidence: 57%

“…A study by Laurent et al 5 evaluated the genotoxicity and cytotoxicity of Ca 3 SiO 5 in addition to its effects on specific functions in target cells. These authors showed that the mortality rate of cells exposed to this cement was similar to that of cells exposed to other biocompatible materials.…”

Section: Discussionmentioning

confidence: 99%

“…An in vivo test can assess the metabolic and pharmacodynamic effects of a product and can help evaluate the influence of gut microbiota on the substance 23, 24 . Therefore, we decided to conduct an in vivo evaluation of the genotoxic and mutagenic properties of Ca 3 SiO 5 . Our results also demonstrate the importance of performing both in vitro and in vivo tests to better evaluate the genotoxic and mutagenic potential of new products.…”

Section: Discussionmentioning

confidence: 99%

“…use to stimulate the proliferation of pulp cells and gingival fibroblasts 5,6,7 . Mori et al 8 evaluated the in vivo biocompatibility of Biodentine and concluded that this cement was not an irritant when in contact with the subcutaneous tissue of rats.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement

et al. 2016

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Ca 3 SiO 5 is new cement based on the composition of Portland that has been developed to have superior physicochemical and biological properties. In a clinical evaluation, the cement did not appear to have cytotoxic properties and allowed for the proliferation of pulp cells and gingival fibroblasts. However, no previous studies have evaluated the genotoxicity or the mutagenicity of Ca 3 SiO 5 in vivo. Therefore, the goal of this study is to evaluate the genotoxic and mutagenic potential of Ca 3 SiO 5 -based cement in vivo. Twenty-four male Wistar rats were divided into 3 groups (n = 8). Group A rats received subcutaneous implantation of Ca 3 SiO 5 in the dorsum. Group B rats received a single dose of cyclophosphamide (positive control). Group C rats received subcutaneous implantation of empty tubes in the dorsum (negative control). After 24 hours, all animals were euthanized and the bone marrow of the femurs was collected for use in the comet assay and the micronucleus test. The comet assay revealed that the Ca 3 SiO 5 group had a tail intensity of 23.57 ± 7.70%, the cyclophosphamide group had a tail intensity of 27.43 ± 7.40%, and the negative control group had a tail intensity of 24.75 ± 5.55%. The average number of micronuclei was 6.25 (standard deviation, SD = 3.53) in the Ca 3 SiO 5 group, 9.75 (SD = 2.49) in the cyclophosphamide group, and 0.75 (SD = 1.03) in the negative control group. There was an increase in the micronuclei frequency in the Ca 3 SiO 5 group compared to that of the negative control group (p < 0.05). Our data showed that exposure to the Ca 3 SiO 5 -based cement resulted in an increase in the frequency of micronuclei, but no genotoxicity was detected according to the comet assay.

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Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement

et al. 2016

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“…One of those materials, Biodentine TM (BD), has become a commercially available dentine substitute. [5] Due to the effects of tricalcium silicate based cements on the proliferation and odontogenic differentiation of human dental pulp cells, they were considered to be ideal capping materials in the primary dentition. [6] In this study, we aimed to compare the clinical and radiographic success of BD, a highly recommended-new material for pulp amputations with FS, which is a frequently used medication in current amputation therapies.…”

Section: Introductionmentioning

confidence: 99%

Clinical Assessment of Pulp Therapy for Primary Molars Performed Under General Anesthesia, Using Two Pulpotomy Agents-A Retrospective Cohort Study

Çarıkçıoğlu¹,

Derelioğlu²,

Yılmaz³

2017

IJDSR

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The aim of this retrospective study is to clinically and radiographically assess the ferric sulfate and tricalcium silicate (Biodentine TM ) pulpotomies. The study consisted a total of 25 children-9 girls and 16 boys aged between 3-6 yrs. From the patients' medical records we determined that 35 primary molars were pulpotomied with Biodentine TM and 65 were pulpotomied with ferric sulfate (FS). Patients were recalled in the post-op 6 th month for clinical examination and in the post-op 12 th month for both clinical and radiographic examination. While a statistically significant difference was found between the factors of age, gender and pulpotomy agents (P<0,05), no statistically significant difference was found between the jaw and teeth types (P>0,05). As a result, recently developed tricalcium silicate based agents (Biodentine TM ) can be used as an alternative to FS in pulpotomy.

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