Induction of systemic resistance in tomato by the autochthonous phylloplane resident Bacillus cereus

Halfeld-Vieira, B. de A.; Júnior, José Roberto Vieira; Silva, Reginaldo Barboza da; Silva, Harllen Sandro Alves; Baracat‐Pereira, Maria Cristina

doi:10.1590/s0100-204x2006000800006

Cited by 49 publications

(25 citation statements)

References 26 publications

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“…These results confirm the antibacterial activity of the sanitizer against the phytobacteria, which was already expected, since quaternary ammonium based compounds possess fungicidal, bactericidal, algicidal and virucidal action (Mcbain et al, 2004;Oosterhof et al, 2006;Abreu et al, 2008). The antagonists probably acted on the reduction of the pathogen population by multiple mechanisms, such as competition for space and nutrients, antibiosis, and, possibly, resistance induction (Lindow & Brandl, 2003;Monier & Lindow, 2004Halfeld-Vieira et al, 2006).…”

Section: Resultssupporting

confidence: 79%

“…Although ISR has not been investigated in this research, Halfeld-Vieira et al (2006) relate the phenomenon for the epiphytic Bacillus cereus in tomato plants, which controlled the bacterium Pseudomonas syringae pv. tomato.…”

Section: Resultsmentioning

confidence: 99%

“…Biosurfactants, antibiotics, bacteriocins and volatile organic compounds (VOCs) synthesis, siderophores and competition for space and nutrients are related to the antagonistic effects of epiphytic bacteria on the phytopathogen growth (Beattie & Lindow, 1999;Lindow & Brandl, 2003). Recent surveys demonstrate that epiphytic bacteria also act as elicitors of the induced systemic resistance (ISR) in plants (Halfeld-Vieira et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Bacterial spot and early blight biocontrol by epiphytic bacteria in tomato plants

Filho

Silva

Alves

2010

Pesq. agropec. bras.

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-The objective of this work was to evaluate in vitro and in vivo biocontrol of bacterial spot (Xanthomonas vesicatoria) and early blight (Alternaria solani) by the epiphytic bacteria Paenibacillus macerans and Bacillus pumilus. Tomato plants were previously sprayed with epiphytic bacteria, benzalkonium chloride and PBS buffer and, after four days, they were inoculated with A. solani and X. vesicatoria. To determine the phytopathogenic bacteria population, leaflet samples were collected from each treatment every 24 hours, for seven days, and plated on semi-selective medium. The effect of epiphytic bacteria over phytopathogens was performed by the antibiosis test and antagonistic activity measured by inhibition zone diameter. The epiphytic and benzalkonium chloride drastically reduced the severity of early blight and bacterial spot in comparison to the control (PBS). In detached leaflets, the epiphytic bacteria reduced in 70% the number of phytopathogenic bacteria cells in the phylloplane. The antibiosis test showed that the epiphytic bacteria efficiently inhibit the phytopathogens growth. In all the bioassays, the epiphytic bacteria protect tomato plants against the phytopathogens.Index terms: Alternaria solani, Bacillus pumilus, Paenibacillus macerans, Xanthomonas vesicatoria, biological control, epiphytic bacteria. Biocontrole da mancha-bacteriana e da pinta-preta por bactérias epifíticas em tomateiro Resumo -O objetivo deste trabalho foi avaliar o biocontrole in vitro e in vivo dos fitopatógenos manchabacteriana (Xanthomonas vesicatoria) e pinta-preta (Alternaria solani) pelas bactérias epifíticasPaenibacillus macerans e Bacillus pumilus. Plantas de tomate foram previamente pulverizadas com as bactérias epifíticas, cloreto de benzalcônio e tampão PBS e, após quatro dias, receberam inoculação com A. solani e X. vesicatoria. A fim de determinar a população da bactéria fitopatogênica, amostras de folíolos foram coletadas de cada tratamento em intervalos de 24 horas, durante sete dias, e inoculadas em meio semisseletivo. O efeito das bactérias epifíticas sobre os fitopatógenos foi realizado pelo teste de antibiose e atividade antagônica avaliada pelo diâmetro da zona de inibição. As bactérias epifíticas e o cloreto de benzalcônico reduziram drasticamente a severidade da pinta-preta e da mancha-bacteriana, comparado com o controle (PBS). Em folíolos destacados, as bactérias epifíticas reduziram em até 70% o número de células da bactéria fitopatogênica no filoplano. As bactérias epifíticas inibem eficientemente o crescimento dos fitopatógenos em meio de cultura. Em todos os bioensaios, as bactérias epifíticas protegem as plantas de tomate contra os fitopatógenos.Termos para indexação: Alternaria solani, Bacillus pumilus, Paenibacillus macerans, Xanthomonas vesicatoria, controle biológico, bactéria epifítica.

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Section: Resultssupporting

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bacterial spot and early blight biocontrol by epiphytic bacteria in tomato plants

Filho

Silva

Alves

2010

Pesq. agropec. bras.

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“…It involves the activation of plant latent defense mechanisms in response to treatment with biotic agents like viable or inactivated microorganisms (STANGARLIN; PASCHOLATI, 1994) or abiotics like aminobutyric acid, 2,6-dichloroisonicotinic acid and benzothiadiazoles (HALFELD-VIEIRA et al, 2006). Plant resistance to pathogens can be and have been obtained from the application of synthetic chemical products on the plants.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of pyraclostrobin and acibenzolar-S-methyl on common bacterial blight of snap bean

Vigo¹,

Maringoni

Camara

et al. 2012

Sem. Ci. Agr.

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Assays were done under greenhouse conditions in order to evaluate the effect of pyraclostrobin (0.0375, 0.0750 and 0.150 mL.L -1 ) and acibenzolar-S-methyl (ASM) (0.025 g.L -1 ) in common bacterial blight on leaves of snap beans cultivar Bragança. These chemicals were sprayed at three different times: five days before; five days before + five days after; and five days after leaf inoculation with an isolate of Xanthomonas axonopodis pv. phaseoli. They were determinate the levels of polyphenoloxidase, peroxidase and total soluble proteins on inoculated and non-inoculated leaves of snap beans sprayed with pyraclostrobin (0.075 g.L -1 ) and ASM (0.025 g.L -1 ). All concentration of pyraclostrobin and ASM reduced the area under the disease progress curve (AUDPC) on leaves of snap beans, and the least AUDPC value was observed when this products were sprayed five days before + five days after inoculation. Higher levels of polyphenoloxidase, peroxidase and the total soluble proteins were observed on leaves sprayed with pyraclostrobin or ASM. Key words: Phaseolus vulgaris, Xanthomonas axonopodis pv. phaseoli, protein ResumoEnsaios foram conduzidos sob condições de casa-de-vegetação para avaliar o efeito de pyraclostrobin (0,0375; 0.0750 e 0,150 mL.L -1 ) e acibenzolar-S-methyl (ASM) (0.025 g.L -1 ) sobre o crestamento bacteriano comum em folhas de feijão-vagem cultivar Bragança. Os produtos foram pulverizados em três diferentes períodos: cinco dias antes, cinco dias antes + cinco dias após e cinco dias após a inoculação dos folíolos com um isolado de Xanthomonas axonopodis pv. phaseoli. Foram determinados os teores de polifenoloxidase, peroxidase e proteínas solúveis totais em folhas inoculadas e não-inoculadas de feijão-vagem pulverizadas com pyraclostrobin (0,075 g.L-1) e ASM (0,025 g.L-1). Todas as concentrações de pyraclostrobin e ASM reduziram os valores da área abaixo da curva do progresso da doença (AUPDC) e o menor valor da AUPDC foi observado para a aplicação dos produtos cinco dias antes + cinco dias após a inoculação. Altos teores de polifenoloxidase, peroxidase e proteínas solúveis totais foram observados nas folhas pulverizadas com pyraclostobin e ASM. Palavras-chave: Phaseolus vulgaris, Xanthomonas axonopodis pv. phaseoli, proteína

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“…Uma estirpe de Bacillus cereus, pulverizada na superfície foliar de tomateiro, foi capaz de reduzir a severidade da mancha-bacteriana pequena, causada por Pseudomonas syringae pv. tomato (Halfeld-Vieira et al, 2006). Em um estudo sobre uma estirpe de B. cereus e uma de Pseudomonas putida, pulverizadas na parte aérea de feijoeiro, observou-se que os dois antagonistas foram capazes de reduzir o progresso da ferrugem (Uromyces appendiculatus) (Vieira Júnior, 2005).…”

Section: Introductionunclassified

Biocontrole da mancha-angular do feijoeiro por antagonistas bacterianos

Garcia

Silva²

2011

Pesq. agropec. bras.

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(2) In memoriam Resumo -O objetivo deste trabalho foi avaliar o efeito de isolados de Bacillus cereus e Pseudomonas putida sobre a severidade da mancha-angular do feijoeiro, em condições de campo. Quatro experimentos foram conduzidos em épocas favoráveis à mancha-angular (Pseudocercospora griseola), com as cultivares Pérola e Ouro Negro. Os experimentos foram realizados em delineamento de blocos ao acaso, com seis repetições. Os tratamentos foram pulverizações sobre as plantas com três estirpes de B. cereus (UFV-172, UFV-101 e UFV-75) e duas estirpes de P. putida (UFV-53 e UFV-Pp), acibenzolar-S-metil, fungicida clorotalonil e controle pulverizado com água, no total de oito tratamentos. Os tratamentos foram aplicados por atomização no filoplano de plantas de feijoeiro, semanalmente, a partir do 15 o dia após a emergência até o fim do ensaio. Calculou-se a área abaixo da curva de progresso da mancha-angular do feijoeiro; a doença ocorreu naturalmente. A produção foi avaliada pela massa média de sementes por planta. Os antagonistas foram eficientes em reduzir a severidade da doença, em comparação à testemunha, e obtiveram o mesmo nível de controle do tratamento químico. O menor índice de severidade da doença propiciou melhor produção do que o tratamento controle.Termos para indexação: Bacillus cereus, Phaseolus vulgaris, Pseudocercospora griseola, Pseudomonas putida. Biocontrol of bean angular leaf spot by bacterial antagonistsAbstract -The objective of this work was to evaluate the effect of Bacillus cereus and Pseudomonas putida isolates on the severity of the angular leaf spot of common bean under field conditions. Four trials were carried out in favorable seasons to angular leaf spot (Pseudocercospora griseola), using the cultivars Pérola and Ouro Negro. The assays were assembled in a randomized block design with six replicates. The treatments were plant-sprayings with three strains of B. cereus (UFV-172, UFV-101 and UFV-75) and two strains of P. putida (UFV-53 and UFV-Pp), acibenzolar-S-methyl, chlorothalonil fungicide and the control with water, in a total of eight treatments. The treatments were applied weekly by spraying the phylloplane of bean plants, starting from the 15 th day after seedling emergency until the end of the assay. The area under the disease progress curve of the angular leaf spot was calculated; the disease occurred naturally. Yield was evaluated by the average seed mass per plant. The antagonists were effective in reducing the severity of the disease, in comparison to the control, and they had the same control level as the chemical treatment. The lowest index of the disease severity provided the best yield, in comparison to the control treatment.

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Induction of systemic resistance in tomato by the autochthonous phylloplane resident Bacillus cereus

Cited by 49 publications

References 26 publications

Bacterial spot and early blight biocontrol by epiphytic bacteria in tomato plants

Bacterial spot and early blight biocontrol by epiphytic bacteria in tomato plants

Evaluation of pyraclostrobin and acibenzolar-S-methyl on common bacterial blight of snap bean

Biocontrole da mancha-angular do feijoeiro por antagonistas bacterianos

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