Induction of T cell-mediated immune response by dendritic cells pulsed with mRNA of sphere-forming cells isolated from patients with gastric cancer

Bagheri, Vahid; Abbaszadegan, Mohammad Reza; Memar, Bahram; Motie, Mohammad Reza; Asmar, M; Mahmoudian, Reihaneh Alsadat; Gholamin, Mehran

doi:10.1016/j.lfs.2019.01.016

Cited by 20 publications

(10 citation statements)

References 34 publications

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“…A study announced that plasmacytoid DCs in peripheral blood and tumor tissue forecasted poor outcomes of GC patients ( Liu X. et al, 2019 ). Whereas, another study indicated DCs loaded with mRNA of CSC-like cells from GC patients might stimulate effective immune responses ( Bagheri et al, 2019 ). Hereto, we could conclude that GCSC markers would affect the clinicopathological features and even prognosis of GC patients by directly affecting cancer cells and modulating the immune microenvironment.…”

Section: Discussionmentioning

confidence: 99%

Human Gastric Cancer Stem Cell (GCSC) Markers Are Prognostic Factors Correlated With Immune Infiltration of Gastric Cancer

Lin

Peng

Mai

et al. 2021

Front. Mol. Biosci.

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The prognosis of patients with gastric cancer (GC) is still unsatisfying. Numerous markers of gastric cancer stem cells (GCSCs) have been identified and were thought to be related to cancer aggressiveness. However, the roles of GCSC markers in GC patients’ prognosis and immune infiltration remain unknown. Expression of GCSC markers was analyzed using Oncomine and Gene Expression Profiling Interactive Analysis (GEPIA). Their associations with clinicopathological parameters were analyzed using UALCAN and LinkedOmics. Alternations and protein expression of GCSC markers were analyzed by cBioPortal and the Human Protein Atlas databases, respectively. The prognostic significance of GCSC markers was evaluated using Kaplan-Meier plotter. Correlations between the expression of GCSC markers and immune infiltration along with biomarkers of tumor-infiltrating immune cells (TIICs) were assessed combined Tumor Immune Estimation Resource and GEPIA. GeneMANIA was used to discover the interactive genes of GCSC markers, and enrichment analysis was performed using Database for Annotation, Visualization, and Integrated Discovery server. We identified six GCSC markers significantly up-expressed in GC, compared with normal stomach tissues. Among them, the overexpression of ICAM1, THY1, and CXCR4 significantly indicated adverse, while EPCAM indicated beneficial clinicopathological features of GC patients. The up-regulation of CXCR4 showed unfavorable prognostic significance, whereas EPCAM and TFRC showed the opposite. The six GCSC markers were all correlated with the infiltration and activation of distinct TIICs. Especially, ICAM1, THY1, and CXCR4 showed strongly positive correlations with tumor-associated macrophages. Besides, chemokine, Toll-like receptor, NF-kappa B, and HIF-1 signaling pathways might be involved in the regulation of GCSC markers on cancer development. This study proposed that GCSC markers might be promising targets of GC treatment to weaken cancer stem-like properties and strengthen anticancer immunity.

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Section: Discussionmentioning

confidence: 99%

Human Gastric Cancer Stem Cell (GCSC) Markers Are Prognostic Factors Correlated With Immune Infiltration of Gastric Cancer

Lin

Peng

Mai

et al. 2021

Front. Mol. Biosci.

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“…Bagheri et al generated DCs by culturing monocytes with GM-CSF plus IL-4 (GM/IL-4 DCs) while maturing them by the cytokine mixture (TNF-α, IL-1β, IL-6, and PEG2). Matured DCs pulsed with CSC mRNA induced Ifng gene expression in T cells ( 15 ). The cytotoxic activity of primed T cells with CSC antigens was significantly enhanced compared to control groups.…”

Section: Advances In Dendritic Cell-based Immunotherapy In Gastric Ca...mentioning

confidence: 99%

Advances in Human Dendritic Cell-Based Immunotherapy Against Gastrointestinal Cancer

2022

Front. Immunol.

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Dendritic cells (DCs), the strongest antigen-presenting cells, are a focus for orchestrating the immune system in the fight against cancer. Basic scientific investigations elucidating the cellular biology of the DCs have resulted in new strategies in this fight, including cancer vaccinology, combination therapy, and adoptive cellular therapy. Although immunotherapy is currently becoming an unprecedented bench-to-bedside success, the overall response rate to the current immunotherapy in patients with gastrointestinal (GI) cancers is pretty low. Here, we have carried out a literature search of the studies of DCs in the treatment of GI cancer patients. We provide the advances in DC-based immunotherapy and highlight the clinical trials that indicate the therapeutic efficacies and toxicities related with each vaccine. Moreover, we also offer the yet-to-be-addressed questions about DC-based immunotherapy. This study focuses predominantly on the data derived from human studies to help understand the involvement of DCs in patients with GI cancers.

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“…Gastric cancer (GC) is one of the five most common cancers worldwide as well as the third biggest cause of cancer-related mortality ( 83 ). Previous studies have shown that pDCs play a crucial role in GC ( 66 ).…”

Section: Role Of Pdcs In Cancersmentioning

confidence: 99%

The Role of Plasmacytoid Dendritic Cells in Cancers

2021

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Plasmacytoid dendritic cells (pDCs) are a special subtype of dendritic cells with the morphology of plasma cells. pDCs produce massive amounts of type I interferon (IFN-I), which was originally found to play an extremely pivotal role in antiviral immunity. Interestingly, accumulated evidence indicates that pDCs can also play an important role in tumorigenesis. In the human body, most of the IFN-α is secreted by activated pDCs mediated by toll-like receptor (TLR) stimulation. In many types of cancer, tumors are infiltrated by a large number of pDCs, however, these pDCs exhibit no response to TLR stimulation, and reduced or absent IFN-α production. In addition, tumor-infiltrating pDCs promote recruitment of regulatory T cells (Tregs) into the tumor microenvironment, leading to immunosuppression and promoting tumor growth. In this review, we discuss recent insights into the development of pDCs and their roles in a variety of malignancies, with special emphasis on the basic mechanisms.

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Induction of T cell-mediated immune response by dendritic cells pulsed with mRNA of sphere-forming cells isolated from patients with gastric cancer

Cited by 20 publications

References 34 publications

Human Gastric Cancer Stem Cell (GCSC) Markers Are Prognostic Factors Correlated With Immune Infiltration of Gastric Cancer

Human Gastric Cancer Stem Cell (GCSC) Markers Are Prognostic Factors Correlated With Immune Infiltration of Gastric Cancer

Advances in Human Dendritic Cell-Based Immunotherapy Against Gastrointestinal Cancer

The Role of Plasmacytoid Dendritic Cells in Cancers

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