Induction of the human CDC45 gene promoter activity by natural compound trans‑resveratrol

Abstract

GGAA motifs in the human TP53 and HELB gene promoters play a part in responding to trans -resveratrol (Rsv) in HeLa S3 cells. This sequence is also present in the 5′-upstream region of the human CDC45 gene, which encodes a component of CMG DNA helicase protein complex. The cells were treated with Rsv (20 µM), then transcripts and the translated protein were analyzed by quantitative RT-PCR and western blotting, respectively. The results showed that the CDC45 gene and protein expression levels were induced after the treatment. To examine whether they were due to the activation of transcription, a 5′-upstream 556-bp of the CDC45 gene was cloned and inserted into a multi-cloning site of the Luciferase (Luc) expression vector. In the present study, various deletion/point mutation-introduced Luc expression plasmids were constructed and they were used for the transient transfection assay. The results showed that the GGAA motif, which is included in a putative RELB protein recognizing sequence, plays a part in the promoter activity with response to Rsv in HeLa S3 cells.