Induction of the SOS response in Escherichia coli by heavy ions

Kozubek, Stanislav; Krasavin, E. A.; Soska, I.; Drasil, V.; Amirtayev, K.G.; Tokarova, B.; Bonev, M.

doi:10.1016/0027-5107(89)90216-9

Cited by 17 publications

(2 citation statements)

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“…An example is given for ␥ rays: in the Ames mutatest, the lower limit of detection, i.e., the dose required to double the number of revertants over the spontaneous level, is in the range of 10 to 15 Gy for S. typhimurium TA98 and TA100 and 3 to 4 Gy for strain TA102 (9,12). Similarly low detection limits are given for the SOS chromotest, with a range of 5 to 10 Gy (10,11,29). With 2.6 Gy for the SOS lux test, the detection limit was even lower than for the SOS chromotest.…”

Section: Discussionmentioning

confidence: 99%

“…This includes the synthesis of a number of proteins involved in mutagenesis, such as RecA and UmuCD (31,38). In the SOS chromotest, Escherichia coli PQ37 cells with the structural gene for ␤-galactosidase, lacZ, under the control of an SOS-controlled gene, sulA, are used as a test system for genotoxicity (11,13,27,29). The umu test makes use of a recombinant S. typhimurium TA 1535(pSK1002) strain with a fused umuC::lacZ operon carried on a plasmid (22)(23)(24).…”

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confidence: 99%

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A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells

Ptitsyn¹,

Horneck²,

Komova³

et al. 1997

Appl Environ Microbiol

141

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A genetically controlled luminescent bacterial reporter assay, the SOS lux test, was developed for rapid detection of environmental genotoxins. The bioassay is based on the recombinant plasmid pPLS-1, which was constructed as a derivative of pBR322, carrying the promoterless luxCDABFE genes of Photobacterium leiognathi downstream of a truncated cda gene from ColD with a strong SOS promoter. E. coli recA ؉ strains containing this construction are inducible to high levels of light production in the presence of substances or agents that cause damage to the DNA of the cells. The light signal, reflecting the SOS-inducing potency, is recorded from the growing culture within 1 s, and the test results are available within 1 to 2 h. Induction of bioluminescence was demonstrated by treatment of E. coli C600(pPLS-1) with 6 genotoxic chemicals (mitomycin C, N-methyl-N-nitro-N-nitrosoguanidine, nalidixic acid, dimethylsulfate, hydrogen peroxide, and formaldehyde) and with UV and ␥ radiation. A clear dose-response relationship was established for all eight genotoxins. The sensitivity of the SOS lux test is similar to that of other bioassays for genotoxicity or mutagenicity, such as the SOS chromotest, umu test, and Ames mutatest. These results indicate that the SOS lux test is potentially useful for the in situ and continuous detection of genotoxins.

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