Induction of tissue-specific stem cells by reprogramming factors, and tissue-specific selection

Noguchi, Hirofumi; Saitoh, Issei; Tsugata, Takako; Kataoka, Hitomi; Watanabe, Masami; Noguchi, Yasufumi

doi:10.1038/cdd.2014.132

Cited by 27 publications

(49 citation statements)

References 38 publications

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“…A recent report has shown that transient expression of the reprogramming factors is able to generate tissue-specific stem cells from pancreatic cells and liver cells (55) …”

Section: Discussionmentioning

confidence: 99%

Induction of Pluripotency in Astrocytes through a Neural Stem Cell-like State

Nakajima-Koyama

Lee

Ohta

et al. 2015

Journal of Biological Chemistry

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It remains controversial whether the routes from somatic cells to induced pluripotent stem cells (iPSCs) are related to the reverse order of normal developmental processes. Specifically, it remains unaddressed whether or not the differentiated cells become iPSCs through their original tissue stem cell-like state. Previous studies analyzing the reprogramming process mostly used fibroblasts; however, the stem cell characteristics of fibroblasts made it difficult to address this. Here, we generated iPSCs from mouse astrocytes, a type of glial cells, by three (OCT3/4, KLF4, and SOX2), two (OCT3/4 and KLF4), or four (OCT3/4, KLF4, and SOX2 plus c-MYC) factors. Sox1, a neural stem cell (NSC)-specific transcription factor, is transiently up-regulated during reprogramming, and Sox1-positive cells become iPSCs. Somatic cells can be reprogrammed into a pluripotent state through ectopic expression of defined transcription factors, such as Oct3/4, Klf4, Sox2, and c-Myc (OKSM) 4 (1). Since the generation of induced pluripotent stem cells (iPSCs), many studies have been done to elucidate the mechanism and nature of molecular changes underlying the reprogramming process (2). However, many questions still remain unanswered. One of the fundamental questions to be answered is whether reprogramming reverses normal developmental processes or not. A recent report showed that human somatic cells are reprogrammed through a mesendoderm-like state (3). In contrast, several reports demonstrated that cells in the intermediate state of fibroblast reprogramming express developmental regulators and cell type-specific genes that are not involved in the fibroblast lineage (4 -6). In addition, several reports have shown transient expression of epidermis genes during fibroblast reprogramming (7-9). These results suggest that the reprogramming process is not simply the reversal of normal developmental processes. However, it remains unclear whether the routes to iPSCs are related to the reverse order of normal developmental processes, and thus, it also remains unknown whether differentiated cells become iPSCs through their original tissue stem cell-like state or not.Most previous studies dealing with the reprogramming process used mouse embryonic fibroblasts (MEFs) (10 -13), which are heterogeneous and contain both mesenchymal and non-mesenchymal cells. Moreover, MEFs themselves are a type of tissue stem cell, and the cell lineage of MEFs is largely unknown. Therefore, the use of MEFs as donor cells may not be suitable for the study addressing the question of whether or not differentiated cells become iPSCs through their original tissue stem cell-like state.It has been reported that various types of cells can be reprogrammed to iPSCs (14). However, the characteristics of intermediate-state cells during reprogramming from donor cell types other than MEFs have been poorly understood. In this study, we used mouse astrocytes. Astrocytes are a type of glial cells, which are differentiated from neural stem cells (NSCs), and embryonic stem cells (ESCs) d...

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“…A recent report has shown that transient expression of the reprogramming factors is able to generate tissue-specific stem cells from pancreatic cells and liver cells (55) …”

Section: Discussionmentioning

confidence: 99%

Induction of Pluripotency in Astrocytes through a Neural Stem Cell-like State

Nakajima-Koyama

Lee

Ohta

et al. 2015

Journal of Biological Chemistry

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“…Cells or cell clumps isolated from a normal pancreas, or those in vitro-produced as a result of transdifferentiation through forced expression of exogenous pancreas/duodenum homeobox protein 1 (Pdx1), Neurogenin-3 (Ngn3), and V-maf musculoaponeurotic fibrosarcoma oncogene homolog A (Mafa) in acinar cells [7] are suitable donors for IPPCT. Tissue-specific stem cells obtained by reprogramming of normal [8] or NOD [9] mouse-derived pancreatic cells, all of which have a potential to produce insulin, would also be suitable donors for IPPCT. Elevated serum levels of insulin, decreased fasting blood glucose levels and recovery of healthy islets in those IDDM model animals may be good indicators of recovery from hyperglycemia associated with IDDM.…”

Section: Experimental Example-2: In Vivo Functional Analysis Of Cellsmentioning

confidence: 99%

Intrapancreatic Parenchymal Cell Transplantation as a Possible Model for the Development of a Cell-based Therapy for Type I Diabetes Mellitus

Inada¹,

Nakamura²,

Saitoh³

2018

obm transplant

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Background: Transplantation of isolated islets is one of the most powerful approaches to cure insulin-dependent (type I) diabetes mellitus (IDDM). Currently, the most widely used transplantation strategy is percutaneous perfusion into the liver via the portal vein. However, this approach has several drawbacks and is often limited by the large number of islets required and hypoxic damage to the grafts. Methods: In this article, we posit intrapancreatic parenchymal cell transplantation (IPPCT) as a promising strategy for cell-based therapy for IDDM. IPPCT is a very simple and convenient

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“…We place such importance on cell adhesion because many molecular biological techniques for cells are performed under cell adhesion conditions. Regenerative medicine associated with the establishment of pluripotent stem cells [9][10][11][12] and gene therapy [13][14][15] may offer new treatments for severe pancreatic diseases, such as diabetes mellitus.…”

Section: Introductionmentioning

confidence: 99%

“…16 It is also difficult to establish pancreatic human induced pluripotent stem cells (iPSCs) and iPSC-like cells. 9,10 Developmentally, both exocrine tissue and pancreatic islets are differentiated from pancreatic buds. Recently, exocrine tissue has been reported to be essential for inducing the differentiation of pancreatic islets.…”

Section: Introductionmentioning

confidence: 99%

Adhesion characteristics of porcine pancreatic islets and exocrine tissue to coating materials

Nakashima

Miyagi-Shiohira

Kobayashi

et al. 2018

Islets

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Since the report of the Edmonton protocol in 2000, islet transplantation has been implemented worldwide, and xenotransplantation using porcine islets has also been reported. In addition, many basic experiments using pancreatic islets and exocrine tissue after isolation have been reported. Recently, exocrine cells have been found to be essential for inducing the differentiation of pancreatic islets. Therefore, the importance of the culture conditions for pancreatic tissue when conducting experiments using pancreatic tissue is also increasing. In this study, we focused on the coat material and examined the adhesive properties of porcine pancreatic islets and exocrine tissue after isolation. Porcine islet isolation was performed, and isolated islets (purity ≥95%) and exocrine tissue (purity ≥99%) were used to achieve adhesion to several extracellular matrixes, fibronectin, collagen type I, collagen type IV, laminin I, fibrinogen, and bovine serum albumin (BSA). DMEM with 0.5% FBS was used as the assay medium. For exocrine tissue, the adhesion was promoted in fibronectin, collagen type I, laminin I, and fibrinogen. The adhesive ability to fibronectin was more than twice that to BSA, while the adhesive ability to collagen type I, laminin I, and fibrinogen was less than twice that to BSA. For islets, the adhesive ability to fibronectin was weaker than that of exocrine tissue. Furthermore, the adhesion effect in fibronectin was obtained within 30 minutes and in medium containing little serum for both islets and exocrine tissues. These data suggest that fibronectin may be useful for the adhesion of pancreatic tissue.

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Induction of tissue-specific stem cells by reprogramming factors, and tissue-specific selection

Cited by 27 publications

References 38 publications

Induction of Pluripotency in Astrocytes through a Neural Stem Cell-like State

Induction of Pluripotency in Astrocytes through a Neural Stem Cell-like State

Intrapancreatic Parenchymal Cell Transplantation as a Possible Model for the Development of a Cell-based Therapy for Type I Diabetes Mellitus

Adhesion characteristics of porcine pancreatic islets and exocrine tissue to coating materials

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