Induction of triploidy in the zebrafish, Brachydanio rerio (Hamilton)

Kavumpurath, Soosamma; Pandian, T. J.

doi:10.1111/j.1365-2109.1990.tb00468.x

Cited by 26 publications

(31 citation statements)

References 15 publications

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“…53 came from diploid fish heterozygous for any one of 14 rp mutations (on 11 different chromosomes), and 49 were isolated from diploid fish homozygous for tp53 M214K . In addition, given that MPNSTs have a near-triploid copy number [43] and triploid zebrafish are viable [46], we also analyzed 45 tumors from triploid tp53 M214K homozygotes to determine whether starting with a triploid genome would alter the genomic content of the resultant tumors. Interestingly, MPNSTs arising in triploid tp53 M214K homozygotes had a pseudo-triploid chromosome number similar to MPNSTs from diploid fish, arguing strongly that this represents the preferred genomic state of this tumor type.…”

Section: Resultsmentioning

confidence: 99%

“…Stocks were maintained as described previously and genotypes were determined by PCR at 8 to 18 weeks of age as described in [67]. Of the zebrafish homozygous for the tp53 M214K point mutation, half were triploid and were made according to previously published methods [46]. Ploidy was determined by measuring DNA content of fish tail cells using propidium iodide (40 µg/ml) staining-based FACS analysis.…”

Section: Methodsmentioning

confidence: 99%

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Comparative Oncogenomic Analysis of Copy Number Alterations in Human and Zebrafish Tumors Enables Cancer Driver Discovery

et al. 2013

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The identification of cancer drivers is a major goal of current cancer research. Finding driver genes within large chromosomal events is especially challenging because such alterations encompass many genes. Previously, we demonstrated that zebrafish malignant peripheral nerve sheath tumors (MPNSTs) are highly aneuploid, much like human tumors. In this study, we examined 147 zebrafish MPNSTs by massively parallel sequencing and identified both large and focal copy number alterations (CNAs). Given the low degree of conserved synteny between fish and mammals, we reasoned that comparative analyses of CNAs from fish versus human MPNSTs would enable elimination of a large proportion of passenger mutations, especially on large CNAs. We established a list of orthologous genes between human and zebrafish, which includes approximately two-thirds of human protein-coding genes. For the subset of these genes found in human MPNST CNAs, only one quarter of their orthologues were co-gained or co-lost in zebrafish, dramatically narrowing the list of candidate cancer drivers for both focal and large CNAs. We conclude that zebrafish-human comparative analysis represents a powerful, and broadly applicable, tool to enrich for evolutionarily conserved cancer drivers.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Comparative Oncogenomic Analysis of Copy Number Alterations in Human and Zebrafish Tumors Enables Cancer Driver Discovery

et al. 2013

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“…The gonads of selected male and female triploids were frozen and sectioned in a freezing microtome (IEC, Needham, MA, U.S.A.). The sections (each 8–10 μm thickness) were stained following standard procedures (Kavumpurath & Pandian, 1990, 1992).…”

Section: Methodsmentioning

confidence: 99%

Maternal and paternal hybrid triploids of tetras

David

Pandian

2006

Journal of Fish Biology

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Hybrid maternal triploids were generated by crossing grey-body and red or yellow-tailed Buenos Aires tetra Hemigrammus caudovittatus (BT) males with albino or black Gymnocorymbus ternetzi [widow tetra (WT)] females and retaining the second polar body by heat shock (HS) or cold shock (CS). Paternal triploids were also generated using 2Á5% polyethylene glycol (PEG) incubated BT semen to facilitate the entry of two sperm into an egg of WT. Optimum temperature for CS was identified as 6°C and the optimum age for thermal shocking the zygote as 3 min after fertilization. At hatching, survival was 25, 17 and 5% for the HS, CS and paternal triploids, respectively. At maturity, it was further reduced to >2% and the maturity inordinately delayed. With the presence of undifferentiated 'steriles' and almost total absence of females, the expected sex ratio was distorted. Triploid males produced spermatocytes, spermatids but not spermatozoa. Triploidy was confirmed by phenotypic markers, karyotyping, erythrocyte measurement and molecular markers. The fusiform body shape of WT was a dominant phenotypic trait over the typical piscine slender body shape of BT. Polymerase chain reaction products of the genomic DNA of the triploids amplified by OPF6 primer were 300, 450 and 1000 bp length, characteristic of BT, and 500 and 800 bp, typical of WT and thereby confirmed the biparental genomic contribution to the triploids. Analyses of genomic DNA of selected progenies using DMRT-1 marker showed that (1) like the diploid BT males, the hybrid triploid males were also true genetic males, and amplified 237 and 300 bp products but (2) the triploid steriles amplified all the three products of 100, 237 and 300 bp indicating that they were mosaics but the diploid steriles failed to amplify.

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“…Ploidy was estimated on the basis of the erythrocyte nucleus area according to Kavumpurath & Pandian (1990) as follows: blood samples were taken from the aorta dorsalis of 122 individuals from nine habitats of natural population of silver crucian carp and were mixed with 3% KCl solution. The nucleus was stained with the fluorescent dye propidium iodide (SIGMA P4170) on a microscopic slide.…”

Section: Methodsmentioning

confidence: 99%

Genetic analysis of offspring from intra‐ and interspecific crosses of Carassius auratus gibelio by chromosome and RAPD analysis

Tóth

Várkonyi

Hidas

et al. 2005

Journal of Fish Biology

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The ploidy of silver crucian carp Carassius auratus gibelio individuals, originating from nine natural habitats of Hungary, was estimated by erythrocyte nucleus area analysis. On the basis of DNA polymorphism, the genetic homogeneity or heterogeneity and the chromosome number of different offspring derived from the crossing of triploid and diploid populations and of two types of silver crucian carp females with other cyprinid males (Cyprinus carpio, Carassius carassius, Carassius auratus and Barbus conchonius) were determined. The results of chromosome and RAPD analysis demonstrated that diploid females could reproduce sexually with silver crucian carp and other cyprinid males and that the offspring of intra-and interspecific crosses contained the paternal DNA. Triploid females usually reproduced by gynogenesis and their offspring were clones, however, in very rare cases paternal genes were actually transmitted (i.e. paternal leakage) to the offspring and the progeny were triploid interspecific hybrids. RAPD analysis showed that while the paternal DNA appeared in the offspring, the maternal phenotype was strongly expressed. # 2005 The Fisheries Society of the British Isles

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Induction of triploidy in the zebrafish, Brachydanio rerio (Hamilton)

Cited by 26 publications

References 15 publications

Comparative Oncogenomic Analysis of Copy Number Alterations in Human and Zebrafish Tumors Enables Cancer Driver Discovery

Comparative Oncogenomic Analysis of Copy Number Alterations in Human and Zebrafish Tumors Enables Cancer Driver Discovery

Maternal and paternal hybrid triploids of tetras

Genetic analysis of offspring from intra‐ and interspecific crosses of Carassius auratus gibelio by chromosome and RAPD analysis

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