Induction strategies in fed-batch cultures for recombinant protein production in Escherichia coli: Application to rhamnulose 1-phosphate aldolase

Process optimization of high-level extracellular production of alkaline pectate lyase in recombinant Escherichia coli BL21 (DE3)

“…Furthermore, it has been reported that the induction method and transitional temperature also lead to changes in the cellular physiology and metabolism [28][29][30]. Thus, in the present study, when the cultivations started to induce at different temperatures, the glycerol feeding rate decreased to 10 or 8 mL/L/h according to its decrease in the glycerol consumption rate when IPTG or lactose was employed as the inducer, respectively.…”

Novel integration strategy coupling codon and fermentation optimization for efficiently enhancing sarcosine oxidase (SOX) production in recombinant Escherichia coli

“…With the dual roles of lactose (inducer and carbon source), the substrate inhibition of high concentration system was also significant, especially in the induction expression phase (Pinsach et al 2008a). Thus, it was essential to maintain a moderate lactose concentration via optimal fedbatch strategy.…”

“…Sequentially, in the fermentation process, how to better balance the cell growth and expression becomes another key problem . Much research suggested that the fermentation efficiency was greatly influenced by the induction conditions, cell growth and substrate inhibition (Donovan et al 1996;Pinsach et al 2008a;Overton 2014). It was reported that the induction conditions were closely related with cell growth and metabolic response (Gombert and Kilikian 1998;Chen et al 2013).…”

Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli

“…A microburette was used for the glucose limiting discrete feeding [2,25]. The feeding was performed such that the specific growth rate ( ) was maintained constant and fixed, through exponential feeding profile previously defined from a model based on mass balances and substrate uptake [3]. Specific growth rate and biomass concentration at the induction moment were varied for each set of experiments as indicated in Section 3.…”

“…Several induction strategies have been proposed, but pulse induction is the most commonly used due to its simplicity [3]. Modeling and optimization of such processes require a mathematical model about inducer transport in order to relate its intracellular cell concentration with the protein production rate.…”