Induction technique of sporulation in vitro of Cercospora beticola

Marcuzzo, Leandro Luiz; Haveroth, Roberto; Nascimento, Andresa Piacezzi

doi:10.1590/0100-5405/2054

Cited by 4 publications

(3 citation statements)

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“…For the Winter 2018 experiment, fungal colonies were grown at 25°C in darkness for 2 weeks. Then the mycelium was inoculated onto a V8 media petri dish (Marcuzzo et al, 2015) to induce sporulation (light intensity of 12.14 µmol s -1 and 25°C). After 1 week, the spores were collected by pouring 10 mL of water with 0.01% Tween 20 onto each plate and gently scraping the spores off with a scalpel.…”

Section: Whole Plant Inoculationmentioning

confidence: 99%

Resistance of garden roses to cercospora leaf spot

Kang¹,

Yan²,

Roundey³

et al. 2019

Acta Hortic.

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Cercospora rosicola is a fungal pathogen that attacks roses and causes spotting on leaves, chlorosis and in severe cases, defoliation. This disease has become more prominent in roses after the widespread use of black spot resistant roses, most likely due to the reduction of fungicide applications on roses. The objective of this thesis is to evaluate garden roses and identify QTLs for resistance to cercospora leaf spot disease. Identification of susceptible and resistant roses to cercospora leaf spot is the first step for breeding cercospora resistant rose cultivars. Cercospora leaf spot was evaluated on a percentage-based rating scale of 0-9 (0=no disease and 9=foliage covered with lesions) during spring, summer and fall of 2016 through 2018 on 130 roses in Overton and College Station, TX. Average cercospora incidence in Overton increased from 2016 to 2017, but decreased in 2018, whereas in College Station, ratings increased three-fold from 2016 (0.6) to 2018 (2.2). Most cultivars showed tolerance to the disease, particularly Rosa rugosa hybrids. However, a number of cultivars including 'American Pillar', 'John Davis', 'Carefree Delight', 'Oso Happy Candy Oh', 'Oso Easy Cherry Pie' and 'Roxanne Veranda' were highly susceptible to cercospora. In College Station, moderately high repeatability and low coefficient of variance occurred in the later months of 2017 and 2018, while in Overton, all the evaluated months in 2016 except for April showed high repeatability and low coefficient of variance. This suggests that ratings during these months are consistent, and may be the more informative months for disease evaluation. Artificial inoculation in a greenhouse setting was also attempted to identify cercospora leaf spot resistant garden roses. Although the pathogen was successfully cultured, the low sporulation, high heat and low humidity during the inoculation iii period slowed disease development. Thus, severe disease symptoms were not observed for this experiment.In 2016, fifteen diploid inter-related rose populations were evaluated for cercospora leaf spot in June, September, October and November in College Station. The estimated broad sense and narrow sense heritability were 0.83 and 0.57, respectively. A pedigree-based analysis using Visual FlexQTL software was conducted on these populations. QTLs found on LG1, LG3 andLG7 appeared in different environments. In the overall analysis, QTLs found on LG1 (0-4cM) and LG3 (36-42cM) explained 8.5% and 7.7% of the total phenotypic variance, respectively.More studies are needed to improve the strength and consistency of QTL detection in this analysis. iv DEDICATION This thesis is dedicated to my father, Yang Woo Kang and my mother, Soo Young Kim for their love and unconditional support. I am also thankful to my sisters Swanie, Serena and Sharon Kang for their patience and encouragement during times of stress. v 2006). Roses are highly heterozygous and prone to moderate inbreeding depression. The inbreeding between modern rose classes may have led to reduced fertility (Zlesa...

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Section: Whole Plant Inoculationmentioning

confidence: 99%

Resistance of garden roses to cercospora leaf spot

Kang¹,

Yan²,

Roundey³

et al. 2019

Acta Hortic.

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“…Soil was infested with R. solani using media containing perlite, corn flour and water in 1:1:5 ratio. Cercospora beticola isolate Ty1, isolated from infected sugar beet leaves grown in Germany, was kept on potato dextrose agar (PDA, DB Difco, Franklin Lakes, NJ, USA) plates at 22 • C in darkness and sporulation was induced on tomato growth extract medium [47]. Botrytis cinerea strain B05.10 [48] was kept on PDA (DB Difco, Franklin Lakes, NJ, USA) and sporulation was induced by 0.5 M NaCl under continuous blue light.…”

Section: Fungal Isolates and Growth Conditionsmentioning

confidence: 99%

The RsRlpA Effector Is a Protease Inhibitor Promoting Rhizoctonia solani Virulence through Suppression of the Hypersensitive Response

Charova

Dölfors

Holmquist

et al. 2020

IJMS

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Rhizoctonia solani (Rs) is a soil-borne pathogen with a broad host range. This pathogen incites a wide range of disease symptoms. Knowledge regarding its infection process is fragmented, a typical feature for basidiomycetes. In this study, we aimed at identifying potential fungal effectors and their function. From a group of 11 predicted single gene effectors, a rare lipoprotein A (RsRlpA), from a strain attacking sugar beet was analyzed. The RsRlpA gene was highly induced upon early-stage infection of sugar beet seedlings, and heterologous expression in Cercospora beticola demonstrated involvement in virulence. It was also able to suppress the hypersensitive response (HR) induced by the Avr4/Cf4 complex in transgenic Nicotiana benthamiana plants and functioned as an active protease inhibitor able to suppress Reactive Oxygen Species (ROS) burst. This effector contains a double-psi beta-barrel (DPBB) fold domain, and a conserved serine at position 120 in the DPBB fold domain was found to be crucial for HR suppression. Overall, R. solani seems to be capable of inducing an initial biotrophic stage upon infection, suppressing basal immune responses, followed by a switch to necrotrophic growth. However, regulatory mechanisms between the different lifestyles are still unknown.

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“…Cendawan genus Cercospora dilaporkan memiliki pertumbuhan yang lambat dengan produksi konidium yang sangat sedikit bila ditumbuhkan pada medium sintetis (Djébali et al 2010;Souza et al 2012;Yuan et al 2015;Uppala et al 2019). Sebagai contoh, pertumbuhan miselium C. beticola membutuhkan waktu lebih dari 20 hari untuk menutupi seluruh cawan petri (80 × 12 mm) dalam inkubasi pada suhu 25 ℃ dengan fotoperiode 12 jam (Marcuzzo et al 2015).…”

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Isolation and Morphological Characterisation of Cercospora janseana Infecting Rice Leaves

Tondok,

Sa’adah

2023

J Fitopatol Indones

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Penyakit bercak cokelat sempit pada tanaman padi menjadi salah satu penyakit yang paling merugikan. Di Indonesia, informasi mengenai penyakit ini masih terbatas dan kurang mendapatkan perhatian petani dan peneliti. Pengetahuan dasar mengenai patogen penyebabnya—Cercospora janseana—diperlukan sebagai acuan dalam tindakan pengendalian dan memahami epidemiologinya di masa mendatang. Penelitian ini bertujuan menentukan teknik isolasi yang sesuai untuk C. janseana serta melakukan karakterisasi morfologinya dari beberapa varietas tanaman padi. Teknik isolasi yang digunakan ialah metode penanaman jaringan, suspensi spora, penyebaran spora, dan penempelan spora. Pengamatan morfologi dilakukan dengan mengamati warna dan pertumbuhan koloni, konidium dan konidiofor, serta pertumbuhan koloni pada medium tumbuh yang berbeda. Hasil penelitian menunjukkan bahwa teknik isolasi yang sesuai untuk C. janseana ialah dengan penempelan spora pada agar-agar air dan menumbuhkannya pada medium agar-agar Martin. Teknik ini lebih baik dibandingkan dengan tiga teknik isolasi lainnya dan berhasil memudahkan proses isolasi C. janseana hingga diperoleh isolat murni.

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Induction technique of sporulation in vitro of Cercospora beticola

Cited by 4 publications

References 0 publications

Resistance of garden roses to cercospora leaf spot

Resistance of garden roses to cercospora leaf spot

The RsRlpA Effector Is a Protease Inhibitor Promoting Rhizoctonia solani Virulence through Suppression of the Hypersensitive Response

Isolation and Morphological Characterisation of Cercospora janseana Infecting Rice Leaves

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