Inductively Coupled Plasma-Mass Spectrometry: Practices and Techniques (Taylor, Howard E.)

Sanders, John K.

doi:10.1021/ed078p1465

Cited by 6 publications

(7 citation statements)

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“…99.998% pure argon gas was used during the analyses by inductively coupled plasma mass spectrometric method. As an internal calibration solution, 1 ml of 10 mg/l solution of indium was used (Thomas 2013;Taylor 2001).…”

Section: Sampling and Analysismentioning

confidence: 99%

Determination of heavy metal background concentration in bottom sediment and risk assessment of sediment pollution by heavy metals in the Hrazdan River (Armenia)

2019

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The purpose of this study is to determine background concentrations of heavy metals in bottom sediments, as well as to characterize potential danger of heavy metals in bottom sediments of the Hrazdan River. Linear regression method was used to determine background concentrations of metals in bottom sediments. While using this method, the linear regression of determining metal with a comparative element was used: The cobalt was chosen as a relative element, which is conditioned by the absence of significant sources of cobalt in this river. Risk assessment index of Hakanson was calculated for evaluation of bottom sediments contamination by heavy metals. It is obtained that in this part of the Hrazdan River basin potential ecological risk caused from bottom sediments contamination by heavy metals is not high.

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Section: Sampling and Analysismentioning

confidence: 99%

Determination of heavy metal background concentration in bottom sediment and risk assessment of sediment pollution by heavy metals in the Hrazdan River (Armenia)

2019

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“…7,8 For instance, inductively coupled plasma mass spectrometry (ICP-MS) can sensitively examine metal and metalloid nanoparticles with sensitive quantitative elemental composition, which is a reliable tool for evaluating the material mass within a defined sample volume, although no information concerning intracellular nanoparticle distribution is available. 9,10 Electron microscopy can resolve electron-dense nanoparticles with a high spatial resolution (1 nm or better), followed by a quantitative analysis via stereology. However, sample preparation and nanoparticle quantification may be time-consuming.…”

mentioning

confidence: 99%

“…In past decades, various nanoparticles have been developed for biomedical applications, including bioimaging, biosensing, and drug delivery. − The question of how many nanoparticles are taken up by cells represents a key aspect not only in the evaluation of the efficiency of nanoparticles in performing the desired action but also in the assessment of biological safety . Different techniques can be used to quantify the cellular uptake of nanoparticles, highly dependent on their inherent nature. , For instance, inductively coupled plasma mass spectrometry (ICP-MS) can sensitively examine metal and metalloid nanoparticles with sensitive quantitative elemental composition, which is a reliable tool for evaluating the material mass within a defined sample volume, although no information concerning intracellular nanoparticle distribution is available. , Electron microscopy can resolve electron-dense nanoparticles with a high spatial resolution (1 nm or better), followed by a quantitative analysis via stereology. However, sample preparation and nanoparticle quantification may be time-consuming.…”

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confidence: 99%

Measuring Cellular Uptake of Polymer Dots for Quantitative Imaging and Photodynamic Therapy

et al. 2021

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There is a great deal of interest in the development of nanoparticles for biomedicine. The question of how many nanoparticles are taken up by cells is important for biomedical applications. Here, we describe a fluorescence method for the quantitative measurement of the cellular uptake of polymer dots (Pdots) and a further estimation of intracellular Pdots photosensitizer for fluorescence imaging and photodynamic therapy. The approach relies on the high brightness, excellent stability, minimal aggregation quenching, and metalloporphyrin doping properties of the Pdots. We correlated the single-cell fluorescence brightness obtained from fluorescence spectrometry, confocal microscopy, and flow cytometry with the number of endocytosed Pdots, which was validated by inductively coupled plasma mass spectrometry. Our results indicated that, on average, ∼1.3 million Pdots were taken up by single cells that were incubated for 4 h with arginine 8-Pdots (40 μg/mL, ∼20 nm diameter). The absolute number of endocytosed Pdots of individual cells could be estimated from confocal microscopy by comparing the single-cell brightness with the average intensity. Furthermore, we investigated the cell viability as a result of an intracellular Pdots photosensitizer, from which the half maximal inhibitory concentration was determined to be ∼7.2 × 105 Pdots per cell under the light dose of 60 J/cm2. This study provides an effective method for quantifying endocytosed Pdots, which can be extended to investigate the cellular uptake of various conjugated polymer carriers in biomedicine.

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“…Introducing ICP-MS in a higher level analytical chemistry class is currently needed because ICP-MS has become one of the most powerful multielement trace analysis techniques used in food, 14,15 agricultural, 16,17 environmental, 18,19 and pharmaceutical analysis. 20,21 The articles published in this journal that have reviewed the principles and applications of ICP-based instrumentation 22,23 have focused only on the use of ICP in total elemental analysis 24−28 and did not include a discussion nor demonstration of metal speciation. Finally, it has been shown that real-life applications of instrumental analysis can stimulate the students' interest and generate lively class discussions to answer relevant day-to-day questions.…”

Section: ■ Introductionmentioning

confidence: 99%

Determination of Total Arsenic and Speciation in Apple Juice by Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry: An Experiment for the Analytical Chemistry Laboratory

Colón

Aga

2016

J. Chem. Educ.

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A two-part laboratory experiment was designed for upper-level analytical chemistry students to provide hands-on experience in the use of high performance liquid chromatography (HPLC) for separation and inductively coupled plasma mass spectrometry (ICP-MS) for detection. In the first part of the experiment, the students analyze total arsenic in apple juice purchased from local grocery stores using direct injection ICP-MS. In the second part, different species of arsenic in the same apple juice samples are determined using HPLC–ICP-MS. Quantification is performed based on two methods, standard addition and external calibration curve, to demonstrate how sample matrix can affect the accuracy of the analysis. The experiments provide the opportunity to introduce and/or review several fundamental analytical chemistry concepts: chromatographic separations, mass spectrometry, inductively couple plasma, matrix effects, calibration methods, internal standard, and basic statistical tests. Undergraduate students enrolled in the Instrumental Analysis Laboratory class at the University at Buffalo performed this experiment successfully during the 2015 spring semester.

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Inductively Coupled Plasma-Mass Spectrometry: Practices and Techniques (Taylor, Howard E.)

Cited by 6 publications

References 0 publications

Determination of heavy metal background concentration in bottom sediment and risk assessment of sediment pollution by heavy metals in the Hrazdan River (Armenia)

Determination of heavy metal background concentration in bottom sediment and risk assessment of sediment pollution by heavy metals in the Hrazdan River (Armenia)

Measuring Cellular Uptake of Polymer Dots for Quantitative Imaging and Photodynamic Therapy

Determination of Total Arsenic and Speciation in Apple Juice by Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry: An Experiment for the Analytical Chemistry Laboratory

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