Induksi Kalus Gaharu dengan NAA dan BAP Secara In Vitro

Wahyuni, Amalina; Satria, Benni; Zainal, Aprizal

doi:10.20961/agsjpa.v22i1.36007

Cited by 5 publications

(8 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Research Karyanti et al (2014) reported the addition of BA 1 mg/L in callus Jatropha curcas L. which was 10 weeks old produced 100% callus sprouts on solid media within 4 weeks after subculture. Furthermore, in the study of Karlianda et al (2010) and Wahyuni et al (2020) stated that the combination treatment of 0.1 mg/L NAA + 2.5 mg/L BAP was the best treatment that produced the highest number of shoots compared to other treatments in the agarwood callus subculture (Aquilaria malaccensis Lamk).…”

Section: Callus Developmentmentioning

confidence: 99%

Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus

et al. 2020

View full text Add to dashboard Cite

Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.

show abstract

Section: Callus Developmentmentioning

confidence: 99%

Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Komposisi media yang tepat juga sangat berpengaruh terhadap biomassa kalus serta metabolit sekunder yang dihasilkan (Pono et al 2021). Faktor ketiga yang mendukung pertumbuhan eksplan adalah zat pengatur tumbuh (ZPT) (Wahyuni et al 2020). Zulkarnain (2011) menyatakan bahwa aplikasi ZPT sangat berpengaruh nyata dalam teknik kultur jaringan.…”

Section: Pendahuluanunclassified

“…Sejalan dengan pernyataan Widiyastuti (2015), pertumbuhan kalus ditandai dengan adanya pembengkakan dan terdapat jaringan berwarna putih kehijauan pada permukaan eksplan. Terbentuknya kalus disebabkan adanya rangsangan luka yang menyebabkan keseimbangan pada dinding sel berubah arah, sebagian protoplas mengalir ke luar sehingga mulai terbentuk kalus (Wahyuni et al 2020). Handoyowati (2016) menyatakan salah satu indikator adanya pertumbuhan dalam kultur jaringan adalah munculnya kalus pada eksplan.…”

Section: Waktu Muncul Kalusunclassified

“…Indah & Ermavitalini (2013a) menyebutkan bahwa tekstur kalus merupakan salah satu penanda yang digunakan untuk menilai pertumbuhan suatu kalus. Struktur kalus remah memiliki ruang antar sel yang besar dan ikatan antar sel yang renggang, sehingga kalus mudah dipisahkan, sedangkan struktur kalus kompak memiliki ikatan antar sel yang padat dan tidak mudah dipisahkan (Wahyuni et al 2020).…”

Section: Warna Dan Tekstur Kalusunclassified

“…Pada | 126 penelitian ini, kalus T. paniculatum menghasilkan tekstur kalus yang kompak pada semua perlakuan. Wahyuni et al (2020) menyatakan tekstur kalus yang kompak merupakan efek dari auksin dan sitokinin yang mempengaruhi potensial air di dalam sel.…”

Section: Warna Dan Tekstur Kalusunclassified

See 2 more Smart Citations

INISIASI KALUS SECARA IN VITRO DARI DAUN Talinum paniculatum (Jacq.) Gaertn.

Afiyah

Surya²,

Ismaini³

et al. 2022

BKR

View full text Add to dashboard Cite

Ginseng Jawa (Talinum paniculatum (Jacq.) Gaertn.) merupakan terna yang daunnya memiliki kandungan metabolit sekunder dan dapat digunakan sebagai obat. Beberapa penelitian kultur jaringan terkait sterilisasi dan penambahan zat pengatur tumbuh pada ginseng Jawa masih belum banyak dilakukan. Tujuan penelitian ini adalah mengetahui metode sterilisasi yang tepat serta melakukan optimalisasi zat pengatur tumbuh Naphthalene Acetic Acid (NAA) dan Benzylaminopurine (BAP) untuk menginisiasi kalus ginseng Jawa. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan Kebun Raya Cibodas – BRIN pada bulan Agustus sampai November 2021. Metode penelitian yang digunakan adalah metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) non faktorial dengan sembilan kombinasi perlakuan penambahan NAA dan BAP yang diulang sebanyak tiga kali. Eksplan daun berdiameter 7 mm ditanam pada botol kultur berisi media Murashige and Skoog yang mengandung kombinasi konsentrasi zat pengatur tumbuh NAA (0,5; 1; dan 1,5 ppm) dan BAP (0,5; 1; dan 1,5 ppm). Hasil penelitian menunjukkan bahwa eksplan yang ditanaman mampu menghasilkan kalus. Waktu kemunculan kalus antara 11-14 hari setelah inisiasi dan memiliki tekstur kompak pada semua perlakuan. Selain itu, empat warna kalus yang dihasilkan dari perlakuan ini, yaitu light olive brown, olive gray, gray brown, dan olive. Kombinasi konsentrasi terbaik diperoleh pada perlakuan N2B2 (1 ppm NAA + 1 ppm BAP). Perlakuan tersebut memberikan pertumbuhan optimal pada parameter panjang kalus sebesar 3,13 cm dan berat basah kalus sebesar 2,27 g.

show abstract

Callus induction of Calotropis gigantea using BAP and 2,4-D in vitro

Muthiah

Sakya

Setyawati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Calotropis gigantea is a plant not widely used but has potential as a medicinal plant. The phytochemical compound can be produced faster using a tissue culture and a combination of growth regulators. The research objectives were to study the effect and determine the best concentration of BAP and 2,4-D in callus induction of Calotropis gigantea. This research was carried out in the Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sebelas Maret University, from September to November 2021. A month-old Calotropis gigantea stem was used as an explant. The research used three replicates of a completely randomized design with 25 combination treatments. The culture medium was MS with BAP (0; 0.5; 1; 1.5; 2 ppm) and 2,4-D (0; 0.5; 1; 1.5; 2 ppm) supplements. Parameters observed in this research were callus formation time, callus colour, and callus texture. The results showed that a combination of BAP 0.5 ppm + 2,4-D 1.5 ppm could induce callus faster than other treatments, 6 days. Callus morphology with green color and compact texture resulted from a combination of BAP 1.5 ppm + 2,4-D 1.5 ppm treatment.

show abstract

Induksi Kalus Gaharu dengan NAA dan BAP Secara In Vitro

Cited by 5 publications

References 2 publications

Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus

Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus

INISIASI KALUS SECARA IN VITRO DARI DAUN Talinum paniculatum (Jacq.) Gaertn.

Callus induction of Calotropis gigantea using BAP and 2,4-D in vitro

Contact Info

Product

Resources

About