Induksi Mutasi Kara Benguk (Mucuna pruriens L.) Menggunakan Iradiasi Sinar Gamma

Saragih, Siti Hartati Yusida; Rizal, Khairul; Sitanggang, Kamsia Dorliana

doi:10.20961/agsjpa.v22i2.44151

Cited by 4 publications

(4 citation statements)

References 2 publications

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“…The comparation of seedling between M1 mutans and Cipedak as a control was indicated that treated seedlings were decrease in the variable of petiole length, leaf blade length, and leaf blade width. The changes in the size of plant organ to become shorter due to mutations caused by gamma ray irradiation also occurred in the study of mutations in winged-bean (Saragih et al, 2020), sunflower (Monikasari et al, 2018), leunca (Saragih & Aisyah, 2019), avocado (El-mageid & Al-Kfrawey, 2018), and mangosteen (Widiastuti et al, 2010). The dendogram of 14 avocado accessions that were tested based on macro-morphological characters showed phenotypic differences up to a coefficient of 33%.…”

Section: Resultsmentioning

confidence: 85%

Effect of Gamma Rays Irradiation to Cipedak Avocado Genetic Diversity

Ihsan¹,

Ashari

Soegianto

et al. 2023

Agrivita.J.Agr.Sci

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Avocado cv. Cipedak is a superior cultivar that match the preferences of Indonesian consumers. However, it has several weaknesses, such as low edible portion, thin skin, and short fruit shelf life. One effort to improve the character weakness is by mutation breeding using gamma ray irradiation. Induction of mutation by gamma ray irradiation at a dose of 10 Gy was conducted on Cipedak avocado shoots in October 2021 which produced 13 first generation avocado mutants (M1). Observation on leaves morphological characters and DNA analysis were needed to find out the changes level of genetical diversity. The research was conducted in Cukurgondang Research Station and Molecular Laboratory of Indonesian Tropical Fruits Research Institute from January to March 2022. The plant material used involved 13 M1 progenies and cv. Cipedak. The material used were 20 weeks old grafted plants. The observed variables were macro and micromorphological characters as well as DNA-RAPD analysis. The results showed that there were phenotypic and genotypic changes in 13 M1 avocados seedlings. The coefficients of differences on macro and micromorphological characters were up to 33% and 28%, respectively and the coefficient of difference for DNA-RAPD analysis was 74%.

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Section: Resultsmentioning

confidence: 85%

Effect of Gamma Rays Irradiation to Cipedak Avocado Genetic Diversity

Ihsan¹,

Ashari

Soegianto

et al. 2023

Agrivita.J.Agr.Sci

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“…However, sword bean seeds had the highest cutting strength value compared to velvet bean and other legume species (Figure 11). Previous studies on velvet beans showed that the LD50 was achieved at 281 Gy, and the highest genetic diversity was obtained at 350 Gy [11]. This means that sword bean need a higher gamma-ray radiation dose than 200 Gy to reach the LD50 and plant genetic diversity.…”

Section: Resultsmentioning

confidence: 91%

“…[13] reported that LD50 on KKL-1 lima bean (Phaseolus lunatus) variety in India was 10 kR (100 Gy). While, LD50 on hyacinth bean was reported by [11] and [12]. There were ranged for 281.5 Gy -512 Gy, depend on the variety.…”

Section: Resultsmentioning

confidence: 99%

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Gamma-Ray Radiation Effect on Two Species of Canavalia Performance

Putri,

Utomo,

Soehendi

2023

IOP Conf. Ser.: Earth Environ. Sci.

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In Indonesia, sword beans are still only occasionally grown since the superior variety is not readily available. An alternative breeding technique to enhance plant characteristics is mutation. The goal of this study was to determine how gamma radiation affected the growth and performance of the two Canavalia species. Four accessions of two Canavalia species each received one of five gamma-ray treatments (0, 50, 100, 150, and 200 Gy). Plant morphology during the germination phase, growth type, plant capacity to produce flowers and pods, flowering age, maturity age, seed weight per plant, seed weight per plot, and seed weight per hundred seeds were all observational factors. Using a factorial randomized complete block design, the data were examined. Microsoft Excel was used for the descriptive analysis, whereas STAR software was used for the analysis of variance and further analyses. Gamma-ray doses of up to 200 Gy have not been successful in altering the characteristics or reaching the LD50 in sword bean. The weight of the seeds per plant and per hundred seeds varied significantly, but the dose and accession variables were inconsistent, making it impossible to determine the ideal combination of the two parameters at this time.

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Effect of gamma irradiation dosage on green bean growth and yield

Supanjani,

Alexander,

Setyowati

2024

BIO Web Conf.

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Gamma radiation is a particular approach for increasing green bean (Vigna radiataL.) growth and yield. Gamma radiation at the appropriate dose enhances plant growth and yield. The research attempts to determine the proper dose of gamma radiation on the growth and yield of green beans. The research was carried out at Talang Kering, Muara Bangkahulu, Bengkulu, Indonesia. The design employed was a Complete Randomized Block Design (RCBD) with two factors. The first factor was the green bean variety, consisting of Vima-1 and Vima-4. The second factor was the radiation dose, which was 0, 50, 100, 200, 400, or 800 Gy. The data were analyzed using the F-test with a 5% significant level and further tested using the Duncan Multiple Range Test (DMRT) at a 5% level. The study's findings revealed that gamma irradiation of up to 800 Gy on green bean seeds resulted in higher plant height, number of pods, number of seeds/plants, and seed weight/plant. The Vima-1 variety of green beans produced a higher weight of 100 seeds when irradiated with gamma rays at 100 Gy, while the Vima-4 variety at a dose of 200 Gy. These findings provide valuable insights for optimizing green bean cultivation practices.

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Induksi Mutasi Kara Benguk (Mucuna pruriens L.) Menggunakan Iradiasi Sinar Gamma

Cited by 4 publications

References 2 publications

Effect of Gamma Rays Irradiation to Cipedak Avocado Genetic Diversity

Effect of Gamma Rays Irradiation to Cipedak Avocado Genetic Diversity

Gamma-Ray Radiation Effect on Two Species of Canavalia Performance

Effect of gamma irradiation dosage on green bean growth and yield

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