Industrial application of cellulases

Rodrigues, Valerie J.; Odaneth, Annamma A.

doi:10.1016/b978-0-12-821882-2.00007-7

Cited by 6 publications

(3 citation statements)

References 70 publications

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“…As reviewed by Ebaid et al. ( 2019 ) and Rodrigues and Odaneth ( 2021 ), most cellulases currently applied for industrial purposes are derived from mesophilic and thermophilic microorganisms. A couple of claimed cold‐active cellulases are commercialized under the names Primafast® GOLD SHL cellulase and IndiAge® NeutraFlex cellulase (Genencor‐DuPont) for treatment of cellulosic fabrics.…”

Section: Discussionmentioning

confidence: 99%

Metagenomic exploration of cold‐active enzymes for detergent applications: Characterization of a novel, cold‐active and alkali‐stable GH8 endoglucanase from ikaite columns in SW Greenland

Oliva,

Zervas,

Stougaard

et al. 2024

Microbial Biotechnology

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Microbial communities from extreme environments are largely understudied, but are essential as producers of metabolites, including enzymes, for industrial processes. As cultivation of most microorganisms remains a challenge, culture‐independent approaches for enzyme discovery in the form of metagenomics to analyse the genetic potential of a community are rapidly becoming the way forward. This study focused on analysing a metagenome from the cold and alkaline ikaite columns in Greenland, identifying 282 open reading frames (ORFs) that encoded putative carbohydrate‐modifying enzymes with potential applications in, for example detergents and other processes where activity at low temperature and high pH is desired. Seventeen selected ORFs, representing eight enzyme families were synthesized and expressed in two host organisms, Escherichia coli and Aliivibrio wodanis. Aliivibrio wodanis demonstrated expression of a more diverse range of enzyme classes compared to E. coli, emphasizing the importance of alternative expression systems for enzymes from extremophilic microorganisms. To demonstrate the validity of the screening strategy, we chose a recombinantly expressed cellulolytic enzyme from the metagenome for further characterization. The enzyme, Cel240, exhibited close to 40% of its relative activity at low temperatures (4°C) and demonstrated endoglucanase characteristics, with a preference for cellulose substrates. Despite low sequence similarity with known enzymes, computational analysis and structural modelling confirmed its cellulase‐family affiliation. Cel240 displayed activity at low temperatures and good stability at 25°C, activity at alkaline pH and increased activity in the presence of CaCl2, making it a promising candidate for detergent and washing industry applications.

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Section: Discussionmentioning

confidence: 99%

Metagenomic exploration of cold‐active enzymes for detergent applications: Characterization of a novel, cold‐active and alkali‐stable GH8 endoglucanase from ikaite columns in SW Greenland

Oliva,

Zervas,

Stougaard

et al. 2024

Microbial Biotechnology

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“…When taking a look at commercial opportunities, cellulases have a well-established market, at USD 1500 million in 2019. It is expected to reach USD 2320 million worldwide by 2024, showing a proportional increase across varied sectors, including animal feed, textile industry, food and beverages, and biofuels [ 182 ]. So far, cellulosomes do not play a significant role in this business.…”

Section: Selected Biotech Tools To Mitigate Ghg Emissions With Major ...mentioning

confidence: 99%

Eight Up-Coming Biotech Tools to Combat Climate Crisis

et al. 2023

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Biotechnology has a high potential to substantially contribute to a low-carbon society. Several green processes are already well established, utilizing the unique capacity of living cells or their instruments. Beyond that, the authors believe that there are new biotechnological procedures in the pipeline which have the momentum to add to this ongoing change in our economy. Eight promising biotechnology tools were selected by the authors as potentially impactful game changers: (i) the Wood–Ljungdahl pathway, (ii) carbonic anhydrase, (iii) cutinase, (iv) methanogens, (v) electro-microbiology, (vi) hydrogenase, (vii) cellulosome and, (viii) nitrogenase. Some of them are fairly new and are explored predominantly in science labs. Others have been around for decades, however, with new scientific groundwork that may rigorously expand their roles. In the current paper, the authors summarize the latest state of research on these eight selected tools and the status of their practical implementation. We bring forward our arguments on why we consider these processes real game changers.

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“…Glucose released from cellulosic hydrolysis could be subsequently used in other bioprocesses to yield valuable end products including amino acids, single cell protein, butanol, ethanol and methane (Ashwini et. al., 2022;Rodrigues and Odaneth, 2021). Cellulases have been used for several years in animals feed preparation, waste-water treatment, detergent formulation and in many different research based sectors.…”

Section: Introductionmentioning

confidence: 99%

Media formulation for cellulase mediated biotransformation of cellulose from wood sawdust waste: Screening, Extracellular production and Optimization of cellulase from a newly isolated Fungal strain C-2 for prospective industrial use

Bibi

Khan

Aslam

2023

Preprint

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Microorganisms populate the most extreme environments due to their incredible capacities of adaptation and biotransformation, based on a genetic potential and biosynthetic pathways of a great diversity. Industrial or agricultural residues are generated in large quantity all over the world and contribute to the global pollution of our biosphere. The current study implies the recycling and the valorization of agro-industrial carbon wastes to produce industrially valuable extracellular cellulase from a newly isolated fungal strain C-2. Cellulase has numerous applications in various food based bioprocesses such as fruit juices clarification and synthesis of single cell protein, glucose syrup, bioethanol and confectioneries. In the present study, fungal strain C-2 extracellularly released high quantity of cellulase at 30°C and pH-03 after 120 min. Among various agro-industrial wastes, 0.2% wood sawdust proved best inducer for cellulase production. It was noticed that the production of cellulase increased when 0.1% yeast extract and ammonium sulfate were used as nitrogen sources. The productivity of cellulase significantly improved after the addition of 0.1% dihydrogen potassium phosphate, 0.2% sodium chloride, 0.002% iron sulfate and 0.02% magnesium sulfate in fermentation medium. It is evident from this study that the newly synthesized cellulase from fungal strain C-2 can be exploited to meet the increasing demand of various industries.

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Industrial application of cellulases

Cited by 6 publications

References 70 publications

Metagenomic exploration of cold‐active enzymes for detergent applications: Characterization of a novel, cold‐active and alkali‐stable GH8 endoglucanase from ikaite columns in SW Greenland

Metagenomic exploration of cold‐active enzymes for detergent applications: Characterization of a novel, cold‐active and alkali‐stable GH8 endoglucanase from ikaite columns in SW Greenland

Eight Up-Coming Biotech Tools to Combat Climate Crisis

Media formulation for cellulase mediated biotransformation of cellulose from wood sawdust waste: Screening, Extracellular production and Optimization of cellulase from a newly isolated Fungal strain C-2 for prospective industrial use

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