Industrial Biocatalysis: an Insight into Trends and Future Directions

Tripathi, Pushplata; Sinha, Sujata

doi:10.1007/s40518-020-00150-8

Cited by 16 publications

(10 citation statements)

References 29 publications

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“…Economic and, increasingly, ecologic criteria play a dominant role when working with enzyme cascades. This includes processes in which few investments have to be made but the highest possible output is produced, ideally in compliance with environmental protection [26]. In this context, a rapid adjustment to market desires is beneficial [27].…”

Section: Optimization Goalsmentioning

confidence: 99%

Getting the Most Out of Enzyme Cascades: Strategies to Optimize In Vitro Multi-Enzymatic Reactions

et al. 2021

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In vitro enzyme cascades possess great benefits, such as their synthetic capabilities for complex molecules, no need for intermediate isolation, and the shift of unfavorable equilibria towards the products. Their performance, however, can be impaired by, for example, destabilizing or inhibitory interactions between the cascade components or incongruous reaction conditions. The optimization of such systems is therefore often inevitable but not an easy task. Many parameters such as the design of the synthesis route, the choice of enzymes, reaction conditions, or process design can alter the performance of an in vitro enzymatic cascade. Many strategies to tackle this complex task exist, ranging from experimental to in silico approaches and combinations of both. This review collates examples of various optimization strategies and their success. The feasibility of optimization goals, the influence of certain parameters and the usage of algorithm-based optimizations are discussed.

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Section: Optimization Goalsmentioning

confidence: 99%

Getting the Most Out of Enzyme Cascades: Strategies to Optimize In Vitro Multi-Enzymatic Reactions

et al. 2021

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“…Bioprocesses are increasingly elaborated in the industrial world and have been already established in several sectors such as agrochemicals, bioremediations, and drug production. 1 Making these processes sustainable is a major issue that scientists try to address. Enzymes' biodegradable and renewable nature offers the major advantage of environmental sustainability and costeffectiveness.…”

Section: ■ Introductionmentioning

confidence: 99%

Development of a ZnO Nanowire Continuous Flow Microreactor with β-Glucosidase Activity: Characterization and Application for the Glycosylation of Natural Products

Gkantzou

Govatsi

Chatzikonstantinou

et al. 2021

ACS Sustainable Chem. Eng.

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Microreactor technology is being increasingly utilized in the field of biocatalysis, offering cost-effectiveness and environmental sustainability. On the other hand, enzyme immobilization on nanomaterials can provide biocatalytic systems with the operational stability demands of an intensified bioprocess. Combining the methodology for ZnO nanowire growth on glass surfaces with enzyme immobilization techniques, we developed a novel continuous-flow microbioreactor system, with β-glucosidase activity. Morphological, spectroscopic, and biocatalytic characterization of the developed enzyme microreactor is presented. The immobilized enzyme exhibited almost 100% remaining hydrolytic activity after 1000 cycles of continuous use and more than 70% residual activity after 24 h of exposure to different organic solvents. The system productivity was enhanced up to 10 times compared to the free enzyme form and 30 times compared to the batch immobilized system. Compared to previous studies in batch reactor systems, the enzyme microbioreactor exhibited a productivity enhancement of 315 and 12 times, respectively, for the continuous glycosylation of the natural compounds perillyl alcohol and tyrosol toward the synthesis of their bioactive glycosylated derivatives. The entire arrangement seems to provide a proper microenvironment for enzyme stabilization, under the scope to broaden the biocatalyst’s potentiality.

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“…The use of biocatalysts in industrial processes is gaining wider acceptance for the purposes of eco-friendliness and cost-effectiveness [21]. Biocatalytic processes meet the needs of sustainability, by development of safer and more environmentally friendly chemistry, which equally integrates the priorities of economic competitiveness and societal concerns, also allowing the design of a new generation of polymers [22,23].…”

Section: Introductionmentioning

confidence: 99%

Achievements and Trends in Biocatalytic Synthesis of Specialty Polymers from Biomass-Derived Monomers Using Lipases

et al. 2021

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New technologies for the conversion of biomass into high-value chemicals, including polymers and plastics, is a must and a challenge. The development of green processes in the last decade involved a continuous increase of the interest towards the synthesis of polymers using in vitro biocatalysis. Among the remarkable diversity of new bio-based polymeric products meeting the criteria of sustainability, biocompatibility, and eco-friendliness, a wide range of polyesters with shorter chain length were obtained and characterized, targeting biomedical and cosmetic applications. In this review, selected examples of such specialty polymers are presented, highlighting the recent developments concerning the use of lipases, mostly in immobilized form, for the green synthesis of e-caprolactone co-polymers, polyesters with itaconate or furan units, estolides, and polyesteramides. The significant process parameters influencing the average molecular weights and other characteristics are discussed, revealing the advantages and limitations of biocatalytic processes for the synthesis of these bio-based polymers.

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Industrial Biocatalysis: an Insight into Trends and Future Directions

Cited by 16 publications

References 29 publications

Getting the Most Out of Enzyme Cascades: Strategies to Optimize In Vitro Multi-Enzymatic Reactions

Getting the Most Out of Enzyme Cascades: Strategies to Optimize In Vitro Multi-Enzymatic Reactions

Development of a ZnO Nanowire Continuous Flow Microreactor with β-Glucosidase Activity: Characterization and Application for the Glycosylation of Natural Products

Achievements and Trends in Biocatalytic Synthesis of Specialty Polymers from Biomass-Derived Monomers Using Lipases

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