Industrial biotechnology goes thermophilic: Thermoanaerobes as promising hosts in the circular carbon economy

Sitara, Angeliki; Hocq, Rémi; Horvath, Josef; Pflügl, Stefan

doi:10.1016/j.biortech.2024.131164

Bioresource Technology

2024

DOI: 10.1016/j.biortech.2024.131164

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Industrial biotechnology goes thermophilic: Thermoanaerobes as promising hosts in the circular carbon economy

Angeliki Sitara,

Rémi Hocq,

Josef Horvath

et al.

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2024

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References 140 publications

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A megatransposon drives the adaptation ofThermoanaerobacter kivuito carbon monoxide

Hocq,

Horvath,

Stumptner

et al. 2024

Preprint

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Acetogens are promising industrial biocatalysts for upgrading syngas, a gas mixture containing CO, H2and CO2into fuels and chemicals. However, CO severely inhibits growth of many acetogens, often requiring extensive adaptation to enable efficient CO conversion (carboxydotrophy). Here, we adapted the thermophilic acetogenThermoanaerobacter kivuito use CO as sole carbon and energy source. Isolate CO-1 exhibited extremely rapid growth on CO and syngas (co-utilizing CO, H2and CO2) in batch and continuous cultures (μmax~ 0.25 h-1). The carboxydotrophic phenotype was attributed to the mobilization of a CO-inducible megatransposon originating from the locus responsible for autotrophy inT.kivui. Transcriptomics illuminated the crucial role maintaining redox balance likely plays during carboxydotrophic growth. These novel insights were exploited to rationally engineerT.kivuito grow on CO. Collectively, our work elucidates a primary mechanism responsible for the acquisition of carboxydotrophy in homoacetogens and showcases how transposons can orchestrate evolution.

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A megatransposon drives the adaptation ofThermoanaerobacter kivuito carbon monoxide

Hocq,

Horvath,

Stumptner

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Industrial biotechnology goes thermophilic: Thermoanaerobes as promising hosts in the circular carbon economy

Cited by 1 publication

References 140 publications

A megatransposon drives the adaptation ofThermoanaerobacter kivuito carbon monoxide

A megatransposon drives the adaptation ofThermoanaerobacter kivuito carbon monoxide

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