Industrial glucoamylase fed‐batch benefits from oxygen limitation and high osmolarity

Pedersen, Lasse Ebdrup; Hansen, Kim Toft; Nielsen, Jens; Lantz, Anna Eliasson; Thykaer, Jette

doi:10.1002/bit.23287

Cited by 23 publications

(31 citation statements)

References 23 publications

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“…For instance, it is necessary to maintain the dissolved oxygen (DO) tension above 30 % during penicillin fermentation; otherwise, the penicillin synthesis is irreversibly affected [23]. While, in other cases, oxygen limitation strategy is very helpful, e.g., during glucoamylase fermentation using Aspergillus niger [24]. Most interesting is the fact that there are some robust strains and wild-type or engineered strains, less sensitive to environmental changes in a certain range, which to a great extent relieves the burden of process optimization and scale-up [25,26].…”

Section: Theory Of Multi-scale Analysis For Fermentation Processmentioning

confidence: 99%

Advances and Practices of Bioprocess Scale-up

Xia

Wang

Lin

et al. 2015

Advances in Biochemical Engineering/Biotechnology

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: This chapter addresses the update progress in bioprocess engineering. In addition to an overview of the theory of multi-scale analysis for fermentation process, examples of scale-up practice combining microbial physiological parameters with bioreactor fluid dynamics are also described. Furthermore, the methodology for process optimization and bioreactor scale-up by integrating fluid dynamics with biokinetics is highlighted. In addition to a short review of the heterogeneous environment in large-scale bioreactor and its effect, a scale-down strategy for investigating this issue is addressed. Mathematical models and simulation methodology for integrating flow field in the reactor and microbial kinetics response are described. Finally, a comprehensive discussion on the advantages and challenges of the model-driven scale-up method is given at the end of this chapter.

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Section: Theory Of Multi-scale Analysis For Fermentation Processmentioning

confidence: 99%

Advances and Practices of Bioprocess Scale-up

Xia

Wang

Lin

et al. 2015

Advances in Biochemical Engineering/Biotechnology

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“…When μ is lower than 0.1 h −1 under chemostat cultivation, q p is coupled with μ. However, when the specific growth rate is higher than 0.1 h −1 , q p will decrease, which indicates that more precursors and energy flow to cell growth (Pedersen et al 2000(Pedersen et al , 2012. So, it was estimated that sufficient supplement of oxygen accelerates the carbon flux flowing to cell growth of A. niger.…”

Section: Introductionmentioning

confidence: 99%

“…At present, the reports on oxygen limitation in fermentation process of filamentous fungi are mainly about the physiological data and metabolomics to describe the parameter changes during the oxygen-limited stage (Pedersen et al 2012;Diano et al 2006Diano et al , 2009Lu et al 2015), while the global response at transcriptional level is not well studied. In this paper, we analyzed the transcriptomic data from samples of different stages in the process of glucoamylase fermentation using a strand-specific RNA-Seq approach.…”

Section: Introductionmentioning

confidence: 99%

“…During the fermentation of filamentous fungi, the viscosity of the medium increased with the increase of biomass (Pedersen et al 2000), which causes the enhancement of transfer resistance and the reduction of oxygen transfer efficiency, and hence inevitably resulted in the oxygen limitation regardless of high power agitation and aeration (Diano et al 2006). Pedersen et al (2012) simulated actual glucoamylase industrial fermentation process for studying the regulation mechanism of physiological metabolism, and it has been observed that during the whole fermentation process, the yield of glucoamylase on substrate was the highest in oxygen limitation phase. Hence, oxygen limitation has been applied as the most effective strategy for industrial glucoamylase production at present.…”

Section: Introductionmentioning

confidence: 99%

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Global transcriptional response of Aspergillus niger in the process of glucoamylase fermentation

Sui

Ouyang

Chu

et al. 2017

Bioresour. Bioprocess.

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Background: In the phase of oxygen limitation during Aspergillus niger fermentation, the cell growth decreased, while the yield of glucoamylase increased continuously and significantly. Explanation on the changes of transcriptome profile during this process may improve our understanding on mechanisms of cell adaption and enzyme production in A. niger. Results:The transcriptomic data from 4 time points in oxygen limitation process of a glucoamylase production A. niger strain were analyzed. Hierarchical clustering of all samples showed that the strongest transcriptional response occurred between the early and middle stage of oxygen limitation. 515 differentially expressed genes (DEGs) were identified and were clustered into 12 expression patterns. Continuously down-regulated DEGs were significantly enriched in GO terms of the ribosome, translation, and aminoacyl-tRNA biosynthesis, and continuously up-regulated DEGs were mainly involved in GO terms of fatty acid catabolism, N-acetyltransferase activity, lipase activity, and carboxylesterase activity. Pyruvate kinase and asparagine synthetase which related to the biosynthesis of the main amino acid composition of the enzyme were significantly up-regulated. Sterol-regulatory element-binding proteins (SREBP) transcription factor SrbB was one of the most up-regulated proteins, indicating its important roles in hypoxia fermentation of A. niger. Conclusion:Comparative transcriptome data analysis of the fermentation revealed that the overall reduced biosynthesis of translation machine and fatty acid, acceleration of fatty acid catabolism, and increased synthesis of main amino acid compositions of glucoamylase are the key transcriptional changes during the oxygen limitation fermentation process of A. niger.

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“…Highly reproducible, replicate bioreactor experiments that provide quality biomass can now be performed with filamentous fungi, thus tackling one of the major challenges in the field. [9][10][11][12][13] It is therefore not a coincidence that the terms Aspergillus and systems biology are now appearing together with greater frequency in the literature, in 83 articles published since 2007, where the first genomes were published. Although the gap between yeast and filamentous fungi remains, it is clear that systems biology approaches in filamentous fungi are increasing exponentially.…”

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confidence: 99%

Integrated Approaches for Assessment of Cellular Performance in Industrially Relevant Filamentous Fungi

WorkmanMhairi

Andersen

ThykaerJette

2013

Industrial Biotechnology

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The performance of filamentous fungi in submerged cultivation determines their suitability for large-scale industrial biotechnology processes and is the result of complex interplay between the physical and chemical parameters of the process and the cellular biology of the fungi. Filamentous fungi have a natural ability to degrade complex substrates through secretion of a large number of diverse enzymes and to produce a number of metabolites that inhibit or prevent the growth of other species in the surroundings. These features have been exploited by industry, resulting in multi-billion dollar processes for producing enzymes and metabolites of value. However, the wealth of diversity of these species is still not fully represented in large-scale bioprocesses, and thus advancement from discovery to application is one of the challenges for modern biotechnology. Acceleration of the process can be achieved through integrated approaches for assessing cellular performance (quantitative physiology), genetic modification of strains (metabolic engineering), and omics analyses and modeling (systems biology). In this review, we evaluate stateof-the-art of filamentous fungal applications in industrial biotechnology , focusing on physiological aspects of the fungi that provide the basis of their cellular performance. We also discuss the advancement of systems biology approaches and how the establishment of these tools for fungal research has begun to reveal the possibilities for further exploitation of these organisms. Increased future focus on multicellular physiology and relevant assays will lead to fungal cells and processes that are customizable to a greater degree, finally allowing the full potential of these complex organisms and their product diversity to unfold.

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Industrial glucoamylase fed‐batch benefits from oxygen limitation and high osmolarity

Cited by 23 publications

References 23 publications

Advances and Practices of Bioprocess Scale-up

Advances and Practices of Bioprocess Scale-up

Global transcriptional response of Aspergillus niger in the process of glucoamylase fermentation

Integrated Approaches for Assessment of Cellular Performance in Industrially Relevant Filamentous Fungi

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