Based on the theory of ecological organization, this paper analyzes the operation mechanism of chemical industry park (CIP) ecosystem by means of dynamic simulation. The research shows that the CIP ecosystem is a complex ecological system whose operation mechanism includes two levels, namely individual enterprises and ecosystem. At the level of individual enterprise, there are competition, symbiosis, invasion and other interactions between enterprises in the CIP ecosystem. Through the pre-determined judgment of the competition effect coefficient and the symbiosis effect coefficient, we calculate how the enterprises influence each other, and then generate their respective operation paths, and finally realize the long-term balance of the system. At the level of ecosystem, the CIP ecosystem makes self-organizing and self-adaptive adjustments and changes due to the changes of the external environment in policy, manpower, technology, capital, market, etc., thus reaching the equilibrium state of coordinated operation and stable symbiosis with the external environment.