Industrial PE-2 strain of Saccharomyces cerevisiae: from alcoholic fermentation to the production of recombinant proteins

Soares-Costa, Andréa; Nakayama, Darlan Gonçalves; Andrade, Letícia de Freitas; Catelli, Lucas Ferioli; Bassi, Ana Karolina Zamprônio; Ceccato-Antonini, Sandra Regina; Henrique-Silva, Flávio

doi:10.1016/j.nbt.2013.08.005

Cited by 7 publications

(6 citation statements)

References 25 publications

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“…The ethanol yield of the PE2 strain was more than double the average calculated across all strains. The PE2 strain has been reported to have a high CO 2 production, final ethanol production (close to 95% of the theoretical yield in E. globulus wood hydrothermal hydrolysate anaerobic fermentation), fast sugar utilization and faster degradation of furfural and 5-hydroxymethyl-2-furaldehyde ( Pereira et al, 2010 , 2014 ; Soares-Costa et al, 2014 ). Furthermore, PE2 has also been shown to dominate and persist in Brazilian distilleries probably because of its high viability and high specific growth rate ( Raghavendran et al, 2017 ; Araújo et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Performance and robustness analysis reveals phenotypic trade-offs in yeast

Trivellin,

Rugbjerg,

Olsson

2023

Life Sci. Alliance

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To design strains that can function efficiently in complex industrial settings, it is crucial to consider their robustness, that is, the stability of their performance when faced with perturbations. In the present study, we cultivated 24Saccharomyces cerevisiaestrains under conditions that simulated perturbations encountered during lignocellulosic bioethanol production, and assessed the performance and robustness of multiple phenotypes simultaneously. The observed negative correlations confirmed a trade-off between performance and robustness of ethanol yield, biomass yield, and cell dry weight. Conversely, the specific growth rate performance positively correlated with the robustness, presumably because of evolutionary selection for robust, fast-growing cells. The Ethanol Red strain exhibited both high performance and robustness, making it a good candidate for bioproduction in the tested perturbation space. Our results experimentally map the robustness–performance trade-offs, previously demonstrated mainly by single-phenotype and computational studies.

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Section: Resultsmentioning

confidence: 99%

Performance and robustness analysis reveals phenotypic trade-offs in yeast

Trivellin,

Rugbjerg,

Olsson

2023

Life Sci. Alliance

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“…Fungi are used in medicine industry to obtain important drugs such as antibiotics, immunosuppressant, vitamins, steroids, and alkaloids. Genetically modified fungi can also be used as bio-factories for the production of numerous enzymes with the most diverse biotechnological applications [ 6 , 7 , 8 ]. However, some fungi can harm human life, causing many infections that can be classified into superficial/subcutaneous (dermatophytosis, pityriasis versicolor, and sporotrichosis) and systemic mycoses (candidiasis, aspergillosis, and cryptococcosis).…”

Section: Introductionmentioning

confidence: 99%

Marine-Derived Compounds and Prospects for Their Antifungal Application

et al. 2020

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The introduction of antifungals in clinical practice has an enormous impact on the provision of medical care, increasing the expectancy and quality of life mainly of immunocompromised patients. However, the emergence of pathogenic fungi that are resistant and multi-resistant to the existing antifungal therapy has culminated in fungal infections that are almost impossible to treat. Therefore, there is an urgent need to discover new strategies. The marine environment has proven to be a promising rich resource for the discovery and development of new antifungal compounds. Thus, this review summarizes more than one hundred marine natural products, or their derivatives, which are categorized according to their sources—sponges, bacteria, fungi, and sea cucumbers—as potential candidates as antifungal agents. In addition, this review focus on recent developments using marine antifungal compounds as new and effective approaches for the treatment of infections caused by resistant and multi-resistant pathogenic fungi and/or biofilm formation; other perspectives on antifungal marine products highlight new mechanisms of action, the combination of antifungal and non-antifungal agents, and the use of nanoparticles and anti-virulence therapy.

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“…The yeast Saccharomyces cerevisiae is a promising unicellular eukaryotic organism for the toxicological evaluation of xenobiotics such as herbicides because its cellular structure and functional organization present many similarities to those of higher-level organisms. Furthermore, S. cerevisiae is a eukaryotic organism that is generally regarded as safe (GRAS), providing for its safe handling during the tests performed in this study [4]. However, the toxicity of phenylurea and triazine herbicides in yeast is still poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Effects of atrazine, isoproturon and diuron on glutathione metabolism of Saccharomyces cerevisiae

Alves-Pereira¹,

Nunes²,

Candeias³

et al. 2015

Green Processing and Synthesis

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Triazines and phenylureas, mainly used in agricultural applications for the selective control of germinating grasses and broad-leaved weeds, are often found in contaminated groundwater, surface water and the effluents of wastewater treatment plants. The toxicity of these herbicides in eukaryotic cells is poorly understood. Saccharomyces cerevisiae is a promising unicellular organism for the toxicological evaluation of xenobiotics because its metabolism is similar to that of higher-level organisms. Consequently, the aim of this study was to compare the effects of three herbicides on yeast-cell growth and the glutathione cycle. Saccharomyces cerevisiae grown in the presence of 5 or 50 μM atrazine, diuron or isoproturon were compared with control cells grown in a rich medium. The results show that the glutathione-dependent buffer capacity decreased significantly in S. cerevisiae grown in the presence of both levels of any of the three herbicides, except in cells exposed to 50 μM isoproturon. In addition, chlorine herbicides inhibited cell growth, probably due to a decrease in antioxidant power and glutathione reductase activity. Isoproturon at 50 μM induced yeast-cell growth, increasing the buffer capacity mediated by glutathione as well as glutathione reductase and glutathione peroxidase activities of UE-ME3 strain. This strain may be useful in studies of isoproturon degradation.

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Industrial PE-2 strain of Saccharomyces cerevisiae: from alcoholic fermentation to the production of recombinant proteins

Cited by 7 publications

References 25 publications

Performance and robustness analysis reveals phenotypic trade-offs in yeast

Performance and robustness analysis reveals phenotypic trade-offs in yeast

Marine-Derived Compounds and Prospects for Their Antifungal Application

Effects of atrazine, isoproturon and diuron on glutathione metabolism of Saccharomyces cerevisiae

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