Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction

Evans, Stephen; Lawrence, Rachel; Ilett, Martha; Burgum, Michael J.; Meldrum, Kirsty; Hondow, Nicole; Jenkins, Gareth; Clift, Martin J. D.; Doak, Shareen H.

doi:10.1016/j.tiv.2022.105415

Cited by 5 publications

(3 citation statements)

References 43 publications

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“…The findings of this study reveal that the occurrence of DNA damage following exposure to ZnO-and Ag-NPs was observed exclusively at cytotoxic concentrations. This contrasts with previously documented outcomes for A549 and TK6 cells, where the same NPs and comet test techniques were employed, yielding essentially identical results [33]. The observed phenomenon can be attributed to the diversity in cell lines, since the HBTEC cells exhibit a lower sensitivity to genotoxic chemicals compared to A549 and TK6 cells.…”

Section: Discussioncontrasting

confidence: 90%

“…After 24 hr of exposure to nanoparticles (5-200 µg/mL), the cytotoxic effect of TiO2-, ZnO-, and AgO-NPs on 2D and 3D HBTEC spheroids was examined using the MTT test to determine the concentration of nanoparticles capable of reaching the ICThe concentration range employed was chosen with both the concentrations of nanoparticles previously observed on 2D bronchial/tracheal cell cultures [32,33] and the typically decreased sensitivity of 3D cultures [34] relative to monolayer cells in consideration. The use of hydrogen peroxide as a positive control was similarly consistent across all three nanoparticles evaluated (Figure 2).…”

Section: Effect Of Nanoparticles Exposure On Cell Viabilitymentioning

confidence: 99%

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Development of Nanoparticles-Induced Genotoxicity in vitro Using Human Airway Epithelial 2D and 3D Spheroid Culture Models

Bordeerat,

Hemmaphan,

Somprasong

2023

Preprint

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New in vitro approaches for the assessment of their toxicity are crucial for creating better risk assessments given the growing use of nanoparticles (NPs) in the production of consumer goods. Humans will be exposed to nanoparticles through the inhalation route from contaminated aerosol. A promising method for evaluating the genotoxicity of NPs in vitro uses human airway epithelial cells. This study aimed to determine whether there were differences in morphology change of human bronchial/tracheal epithelial (HBTEC) cells responded to NPs in 2D and 3D spheroid models and to evaluate genotoxicity in 2D and 3D cultures after 24 hr exposure to TiO2-, Ag- and ZnO-NPs using the comet assay. Our 2D and 3D HBTEC cultures with TiO2-NPs did not show any statistically significant genotoxicity compared to controls for concentration points tested in vitro. HBTEC cells grown as 2D indicated that exposure to ZnO-NPs at low concentrations (10-50 ug/mL) for 24 hr did not cause any genotoxic effect but, at high concentrations, genotoxicity was observed. In contrast, treatment of 2D HBTEC with AgO-NPs led to an elevated in percentage of tail DNA damage from 50 ug/mL to 100 ug/mL, which was concentra-tion-dependent. Using 3D spheroid assays revealed a lower %tail length in ZnO- and AgO-NPs, thus exhibiting in vitro genotoxicity of NPs in this cell model. Spheroids (3D) rather than mono-layer cultures (2D) are believed to more precisely represent in vivo-like cell behavior when evaluating genotoxicity. Together, our findings demonstrate the genotoxic effects of NPs and point to the critical role that cell culture models play in the evaluation of toxicological risk.

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Section: Discussioncontrasting

confidence: 90%

Section: Effect Of Nanoparticles Exposure On Cell Viabilitymentioning

confidence: 99%

Development of Nanoparticles-Induced Genotoxicity in vitro Using Human Airway Epithelial 2D and 3D Spheroid Culture Models

Bordeerat,

Hemmaphan,

Somprasong

2023

Preprint

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“…Cytotoxicity was determined by the physicochemical properties, exposure concentration and time of nanoparticles [ 23 , 41 ]. Coarse particles of titanium dioxide or nanoparticles exposed to low doses for long-term exposure have also shown low cytotoxicity [ 42 , 43 ]. The mechanism of cytotoxicity may be related to oxidative stress, inflammatory response and genotoxicity induced by TiO 2 NPs [ 29 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

DNA Oxidative Damage as a Sensitive Genetic Endpoint to Detect the Genotoxicity Induced by Titanium Dioxide Nanoparticles

et al. 2022

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The genotoxicity of nanomaterials has attracted great attention in recent years. As a possible occupational carcinogen, the genotoxic effects and underlying mechanisms of titanium dioxide nanoparticles (TiO2 NPs) have been of particular concern. In this study, the effect of TiO2 NPs (0, 25, 50 and 100 µg/mL) on DNA damage and the role of oxidative stress were investigated using human bronchial epithelial cells (BEAS-2B) as an in vitro model. After detailed characterization, the cytotoxicity of TiO2 NPs was detected. Through transmission electron microscopy (TEM), we found that TiO2 NPs entered the cytoplasm but did not penetrate deep into the nucleus of cells. The intracellular levels of reactive oxygen species (ROS) significantly increased in a dose-dependent manner and the ratios of GSH/GSSG also significantly decreased. The results of the normal comet assay were negative, while the Fpg-modified comet assay that specifically detected DNA oxidative damage was positive. Meanwhile, N-acetyl-L-cysteine (NAC) intervention inhibited the oxidative stress and genotoxicity induced by TiO2 NPs. Therefore, it was suggested that TiO2 NPs could induce cytotoxicity, oxidative stress and DNA oxidative damage in BEAS-2B cells. DNA oxidative damage may be a more sensitive genetic endpoint to detect the genotoxicity of TiO2 NPs.

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Environmental genotoxicity assessment of nanoparticles using human airway epithelial model

Hemmaphan,

Somprasong,

Bordeerat

2024

Emerging Contaminants

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Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction

Cited by 5 publications

References 43 publications

Development of Nanoparticles-Induced Genotoxicity in vitro Using Human Airway Epithelial 2D and 3D Spheroid Culture Models

Development of Nanoparticles-Induced Genotoxicity in vitro Using Human Airway Epithelial 2D and 3D Spheroid Culture Models

DNA Oxidative Damage as a Sensitive Genetic Endpoint to Detect the Genotoxicity Induced by Titanium Dioxide Nanoparticles

Environmental genotoxicity assessment of nanoparticles using human airway epithelial model

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