Industrial-Scale Production of Inactivated Poliovirus Vaccine Prepared by Culture of Vero Cells on Microcarrier

Montagnon, B.; Fanget, B.; Vincent-Falquet, J. C.

doi:10.1093/clinids/6.supplement_2.s341

Cited by 76 publications

(20 citation statements)

References 2 publications

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“…The production systems generally used for anchorage-dependent cells are those providing large surfaces. Apart from the fluidized bed reactor 36,37 and the microcarrier culture systems, 38 which have a large scale-up potential, all other reactor systems developed for surface attached growth (CellCube, hollow fiber system, fixed bed reactor system) are limited in their scale. The use of suspension culture systems (stirred tank reactor system) has the main advantage of an almost unlimited potential of scale-up which is proven in theory and practice.…”

Section: Discussionmentioning

confidence: 99%

Development of a suspension packaging cell line for production of high titre, serum-resistant murine leukemia virus vectors

Pizzato

Merten

Blair³

et al. 2001

Gene Ther

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Section: Discussionmentioning

confidence: 99%

Development of a suspension packaging cell line for production of high titre, serum-resistant murine leukemia virus vectors

Pizzato

Merten

Blair³

et al. 2001

Gene Ther

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“…This culture was certified by the World Health Organization for the manufacture of viral vaccines [12]. Proliferative activity of cells remained high during culturing in nutrient media with 5% FBS.…”

Section: Resultsmentioning

confidence: 99%

Morphological and Proliferative Characteristics of Vero and MDCK Cells during Culturing in Nutrient Media on the Basis of Hydrolysates of Plant Proteins

et al. 2009

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Morphological and proliferative characteristics of cultured Vero and MDCK cells were compared after growth in nutrient media of the basis of enzymatic hydrolysates of rice flour proteins and soybean flour proteins or control media (DMEM and Axcevir-MDCK). These media had a strong stimulatory effect on the growth of cultured Vero cells (addition of 3% fetal bovine serum, Gibco), but did not modulate the morphology of this culture. Culturing of MDCK cells in nutrient media on the basis of enzymatic hydrolysates of rice and soybean flour proteins with low content of fetal bovine serum (2%, Gibco) was accompanied by insignificant changes in the index of proliferation and morphological characteristics of cultured cells.

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“…Several unit operations have been described for the removal of solid matter from virus-containing cell culture supernatant: microfiltration [42], depth filtration [43,44] and centrifugation [11,43,45,46]. In the present study, clarification in batch centrifuges was evaluated as a model system for the clarification in manufacturing-scale continuous centrifuges.…”

Section: Centrifugationmentioning

confidence: 99%

DNA Depletion by Precipitation in the Purification of Cell Culture‐Derived Influenza Vaccines

et al. 2010

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A pilot study for the purification of cell culture-derived human influenza virus is presented, which focuses on the early removal of DNA by precipitation. Strains of influenza virus were propagated using Madin Darby canine kidney cells as a host. A harvesting time of about 72 h postinfection was chosen to minimize the level of impurities (host cell DNA and protein). Cell culture supernatant was clarified by centrifugation and the performance of this operation was characterized on the basis of Sigma theory. An average clarification efficiency of 93 % (based on turbidity) and a product yield of 85 % (based on hemagglutination activity) were obtained at a load of 1.6 · 10 -8 m s -1. Furthermore, the applicability of Sigma theory for scale-up studies using two different laboratory centrifuges was verified. Selective precipitation of DNA was achieved by the addition of polyethyleneimine (PEI). Full factorial design was applied to optimize selectivity considering pH, ionic strength, and the concentration and molecular weight of PEI. Under optimized conditions, treatment with PEI resulted in a reduction of DNA to 15 % of the initial amount, while 86 % of virions (based on neuraminidase activity) were recovered. The subsequent concentration of virions was realized by tangentialflow ultrafiltration. Recovery based on hemagglutination activity was determined to 63 % on average. Including the previous precipitation step, overall reduction in DNA after tangential-flow ultrafiltration was 500-fold. These results indicate that the suggested unit operations are suited for the early depletion of DNA in cell culture-derived influenza vaccine production.

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Industrial-Scale Production of Inactivated Poliovirus Vaccine Prepared by Culture of Vero Cells on Microcarrier

Cited by 76 publications

References 2 publications

Development of a suspension packaging cell line for production of high titre, serum-resistant murine leukemia virus vectors

Development of a suspension packaging cell line for production of high titre, serum-resistant murine leukemia virus vectors

Morphological and Proliferative Characteristics of Vero and MDCK Cells during Culturing in Nutrient Media on the Basis of Hydrolysates of Plant Proteins

DNA Depletion by Precipitation in the Purification of Cell Culture‐Derived Influenza Vaccines

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