2017
DOI: 10.1002/smll.201700705
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Inertial Microfluidic Cell Stretcher (iMCS): Fully Automated, High‐Throughput, and Near Real‐Time Cell Mechanotyping

Abstract: Mechanical biomarkers associated with cytoskeletal structures have been reported as powerful label-free cell state identifiers. In order to measure cell mechanical properties, traditional biophysical (e.g., atomic force microscopy, micropipette aspiration, optical stretchers) and microfluidic approaches were mainly employed; however, they critically suffer from low-throughput, low-sensitivity, and/or time-consuming and labor-intensive processes, not allowing techniques to be practically used for cell biology r… Show more

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Cited by 68 publications
(81 citation statements)
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“…The simplicity, controllability, versatility, and biocompatibility of the newly described technology make it highly suitable for multiple applications. These include important applications in physics (e.g., studying complex fluids and soft materials [5][6][7] and generation of droplets [58,59] ), chemistry (e.g., synthesis of various molecules and compounds [11,12] ), and biology (e.g., manipulation of cells, [60,61] performing multistep assays, [13] diagnostics, [62] and developing organ-on-a-chip platforms [63] for studying the mechanobiology of cells [64,65] and the human circulatory system [17,19,66] ). Notably, both customized harmonic and disturbed flow patterns can be created and studied using the newly described technology.…”
Section: Resultsmentioning
confidence: 99%
“…The simplicity, controllability, versatility, and biocompatibility of the newly described technology make it highly suitable for multiple applications. These include important applications in physics (e.g., studying complex fluids and soft materials [5][6][7] and generation of droplets [58,59] ), chemistry (e.g., synthesis of various molecules and compounds [11,12] ), and biology (e.g., manipulation of cells, [60,61] performing multistep assays, [13] diagnostics, [62] and developing organ-on-a-chip platforms [63] for studying the mechanobiology of cells [64,65] and the human circulatory system [17,19,66] ). Notably, both customized harmonic and disturbed flow patterns can be created and studied using the newly described technology.…”
Section: Resultsmentioning
confidence: 99%
“…By controlling the inertial migration of the cells, manipulation of the cells with different sizes can be realized. In practical bioengineering applications, the cells that need to be treated are generally non‐spherical, including discoid red cells, columnar Enterobacteriaceae, ellipsoid algae and so on .…”
Section: Cell Manipulation Approaches By Dielectrophoresismentioning
confidence: 99%
“…Similarly, Deng et al developed a fully automated high-throughput device to measure cell deformability as a metric of breast cancer cell progression to provide new information about distinctive changes in different breast cancer cell lines including MDA-MB-231 and MCF7. Additionally, their device was capable of identifying cell stiffness change that occurred during EMT [ 82 ]. The measurement of cellular electrical properties (e.g., impedance) combined with the quantification of the mechanical properties can provide additional information about the cellular phenotype as another metric to characterize cell heterogeneity.…”
Section: Disease Diagnosticsmentioning
confidence: 99%