Inertial microfluidics in parallel channels for high-throughput applications

Hansson, Jonas; Karlsson, Johannes; Haraldsson, Tommy; Brismar, Hjalmar; Wijngaart, Wouter van der; Russom, Aman

doi:10.1039/c2lc40241f

Cited by 53 publications

(38 citation statements)

References 26 publications

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“…3 New applications have since emerged, with inertial focusing in particular, leading to new methods for high-throughput cytometry. [4][5][6][7][8][9][10] More recently, fluid sculpting via inertial flow has been demonstrated through so-called "pillar programming." 11,12 See Fig.…”

Section: Introductionmentioning

confidence: 99%

Optimization of micropillar sequences for fluid flow sculpting

Stoecklein

Kim

et al. 2016

Physics of Fluids

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Inertial fluid flow deformation around pillars in a microchannel is a new method for controlling fluid flow. Sequences of pillars have been shown to produce a rich phase space with a wide variety of flow transformations. Previous work has successfully demonstrated manual design of pillar sequences to achieve desired transformations of the flow cross section, with experimental validation. However, such a method is not ideal for seeking out complex sculpted shapes as the search space quickly becomes too large for efficient manual discovery. We explore fast, automated optimization methods to solve this problem. We formulate the inertial flow physics in microchannels with different micropillar configurations as a set of state transition matrix operations. These state transition matrices are constructed from experimentally validated streamtraces for a fixed channel length per pillar. This facilitates modeling the effect of a sequence of micropillars as nested matrix-matrix products, which have very efficient numerical implementations. With this new forward model, arbitrary micropillar sequences can be rapidly simulated with various inlet configurations, allowing optimization routines quick access to a large search space. We integrate this framework with the genetic algorithm and showcase its applicability by designing micropillar sequences for various useful transformations. We computationally discover micropillar sequences for complex transformations that are substantially shorter than manually designed sequences. We also determine sequences for novel transformations that were difficult to manually design. Finally, we experimentally validate these computational designs by fabricating devices and comparing predictions with the results from confocal microscopy. C 2016 AIP Publishing LLC. [http://dx

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Section: Introductionmentioning

confidence: 99%

Optimization of micropillar sequences for fluid flow sculpting

Stoecklein

Kim

et al. 2016

Physics of Fluids

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“…34 In a low AR (AR < 1) straight microchannel, Hansson et al demonstrated an even higher efficiency (g > 95%) using 10 lm diameter particles. 35 In terms of collecting large cells, a low AR channel is preferred as the target cells can be collected from a single central outlet, rather than from the two side outlets as in high AR microchannels. While these systems offer good performance in filtration of particles, parameters that are critical in downstream cell analysis, such as viability and proliferation of collected cells, are yet to be explored.…”

Section: Introductionmentioning

confidence: 99%

Modulation of rotation-induced lift force for cell filtration in a low aspect ratio microchannel

et al. 2014

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Cell filtration is a critical step in sample preparation in many bioapplications. Herein, we report on a simple, filter-free, microfluidic platform based on hydrodynamic inertial migration. Our approach builds on the concept of two-stage inertial migration which permits precise prediction of microparticle position within the microchannel. Our design manipulates equilibrium positions of larger microparticles by modulating rotation-induced lift force in a low aspect ratio microchannel. Here, we demonstrate filtration of microparticles with extreme efficiency (>99%). Using multiple prostate cell lines (LNCaP and human prostate epithelial tumor cells), we show filtration from spiked blood, with 3-fold concentration and >83% viability. Results of a proliferation assay show normal cell division and suggest no negative effects on intrinsic properties. Considering the planar low-aspect-ratio structure and predictable focusing, we envision promising applications and easy integration with existing lab-on-a-chip systems. V C 2014 AIP Publishing LLC.

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“…All together, the reported straight channel aspect ratio has been limited to 1:3, with the largest side channel being 100 lm and a maximum flow rate of 200 ll/min. 15,21,22 In this work, we introduce flow through u-shaped channels and demonstrate particle focusing into a single and deterministic lateral position that enables effective extraction of the focused stream at more than 20 times higher flow rate compared to straight channels.…”

Section: High Throughput Particle and Cell Filtrationmentioning

confidence: 99%

“…This is in agreement with our previous work in flow through straight channels up to AR 1:3. 22 In both cases, the particles are focused into a single lateral focusing position when exiting the curvature. In flow through straight, square, and rectangular channels, the absence of particles in the corners in AR $ 1:1 suggests that additional lateral migration effects take place near channel walls.…”

Section: A Flow Through Curved Channelsmentioning

confidence: 99%

“…12 The focusing position can be reduced to two positions located centrally along the longer channel dimension in straight rectangular channels, and have been used to continuously focus and filter particles based on size. 15,21,22 Different curved geometries have been explored to reduce the focusing points, including asymmetrically repetitive curves, 12 spirals, 16,17 and contraction-expansion microchannels. 23 In flow through curved channel geometries, curvature amplifies a lateral instability that drives a secondary cross-sectional flow field (Dean flow) characterized by the presence of two counter-rotating vortices located above and below the horizontal plane of symmetry of the channel.…”

Section: Introductionmentioning

confidence: 99%

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Dean flow-coupled inertial focusing in curved channels

et al. 2014

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Passive particle focusing based on inertial microfluidics was recently introduced as a high-throughput alternative to active focusing methods that require an external force field to manipulate particles. In inertial microfluidics, dominant inertial forces cause particles to move across streamlines and occupy equilibrium positions along the faces of walls in flows through straight micro channels. In this study, we systematically analyzed the addition of secondary Dean forces by introducing curvature and show how randomly distributed particles entering a simple u-shaped curved channel are focused to a fixed lateral position exiting the curvature. We found the lateral particle focusing position to be fixed and largely independent of radius of curvature and whether particles entering the curvature are pre-focused (at equilibrium) or randomly distributed. Unlike focusing in straight channels, where focusing typically is limited to channel cross-sections in the range of particle size to create single focusing point, we report here particle focusing in a large cross-section area (channel aspect ratio 1:10). Furthermore, we describe a simple u-shaped curved channel, with single inlet and four outlets, for filtration applications. We demonstrate continuous focusing and filtration of 10 lm particles (with >90% filtration efficiency) from a suspension mixture at throughputs several orders of magnitude higher than flow through straight channels (volume flow rate of 4.25 ml/min). Finally, as an example of high throughput cell processing application, white blood cells were continuously processed with a filtration efficiency of 78% with maintained high viability. We expect the study will aid in the fundamental understanding of flow through curved channels and open the door for the development of a whole set of bio-analytical applications. V C 2014 AIP Publishing LLC.

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Inertial microfluidics in parallel channels for high-throughput applications

Cited by 53 publications

References 26 publications

Optimization of micropillar sequences for fluid flow sculpting

Optimization of micropillar sequences for fluid flow sculpting

Modulation of rotation-induced lift force for cell filtration in a low aspect ratio microchannel

Dean flow-coupled inertial focusing in curved channels

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