Inexpensive fed-batch cultivation for high poly(3-hydroxybutyrate) production by a new isolate of Bacillus megaterium

Efficient polyhydroxybutyrate production from Bacillus thuringiensis using sugarcane juice substrate

“…B. thuringiensis B417-5 grew slowly in nitrogen-deficient medium containing soluble starch and sugarcane molasses as the carbon source, perhaps due to the low amylase activity of this isolate or perhaps due to the effect of toxic compounds, such as furfural and phenolic compounds in the sugarcane molasses (Takara et al, 2007). Our results contradicted those of Gowda and Shivakumar (2014), who reported that B. thuringiensis IAM 12077 produced a high amount of PHB in a medium using starch as the carbon source (2.8 g/L, 41.50% of DCW), and Kulpreecha et al (2009), who reported that B. megaterium BA-019 produced a high amount of PHB (4.16 g/L, 50.50% of DCW) in a medium using 2% (w/v) sugarcane molasses as the carbon source. However, B. thuringiensis R1 was reported to produce a low amount of PHB (0.72 g/L, 23.06% of DCW) when using 1% (w/v) sugarcane molasses as the carbon source (Rohini et al, 2006).…”

Exploring biodegradable polymer production from marine microbes

“…A wide range of industrial by-products has been used for PHAs production like agricultural, household waste materials, sugars, lignocellulosic raw materials, fats, and oils. Among those, extensive research is focused on wastes, sucrose [108], starch [109], glucose [110][111][112], soy molasses and hydrolysed soy [113,114], sugar cane molasses [115][116][117], waste rapeseed oil [118,119], sunflower meal hydrolysates [120], glycerol [121,122], rice bran and corn starch [74], lard oil, butter oil, and coconut oil [123], palm oil and its products [124], sugar beet molasses [125], spent palm oil [126], cellulose and cellulose hydrolysates [127], sugarcane bagasse hydrolysates [128,129], casein hydrolysate [130], rapeseed meal hydrolysates [131], triacylglycerides (TAG) [132], sugarcane liquor [133], acetic acid [111,134], corn steep liquor [135], fish peptone medium [136], galactose, mannose and rhamnose [137], cheese whey and hydrolysed whey [115,138,139], urea [117], oil [124], wheat based biorefinery [140,141], xylose [128], arabinose …”

Utilization of palm oil mill effluent for polyhydroxyalkanoate production and nutrient removal using statistical design

“…The local desirability (d) in this study was Table 4 Statistical analysis and numerical optimization of different factors used in the optimization study for the PHAs production, organic, and nutrient removal in the fed-batch reactor using Therefore, the curve line has a sharp shape compared to other influence factors. As a conclusion, carbon ratio must be well controlled in order to reach both PHA production and nutrient removal (Kulpreecha et al 2009). At the same time, the study also suggests that the AFR, CL and SFR should be operated at 0.59 L/min, 20 h, and 22 mL/min, respectively.…”