2021
DOI: 10.1101/2021.08.18.21256786
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Inexpensive workflow for simultaneous monitoring of HIV viral load and detection of SARS-CoV-2 infection

Abstract: Background: COVID-19 pandemic interrupted routine care for individuals living with HIV, putting them at risk of becoming virologically unsuppressed and ill. Often they are at high risk for exposure to SARS-CoV-2 infection and severe disease once infected. For this population, it is urgent to closely monitor HIV plasma viral load (VL) and screen for SARS-COV-2 infection. Method: We have developed a non-proprietary method to isolate RNA from plasma, nasal secretions (NS), or both. HIV, SARS-CoV-2, and human RP t… Show more

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Cited by 3 publications
(2 citation statements)
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“…RNA at 10 10 copies/l was stored in nuclease-free water in single-use aliquots. SARS-CoV-2 RNA was prepared and quantified as previously described (35,36).…”
Section: Methodsmentioning
confidence: 99%
“…RNA at 10 10 copies/l was stored in nuclease-free water in single-use aliquots. SARS-CoV-2 RNA was prepared and quantified as previously described (35,36).…”
Section: Methodsmentioning
confidence: 99%
“…5 μL of extracted RNA was added to the 15 μL RT-PCR mixture and subjected to 5 min at 55°C, 1 min of 94°C and 45 cycles of 1 s 94°C and 30 s at 57°C and read using FAM channel on a CFX96. Each clinical sample was run with one technical replicate for each N1, N2, or RP assay, along with standards using synthetic RNA templates prepared in-house and quantified using ddPCR as described ( 35 ). Cq and SQ values were exported from Bio-Rad CFX Maestro 1.1 software using the RFU threshold of 50 across all data sets.…”
Section: Methodsmentioning
confidence: 99%