Infant Exposure to Bisphenol A Can Be Quantitatively Assessed by a Simply Improved High-Performance Liquid Chromatography–Tandem Mass-Spectrometry Method

Abstract

To quantitatively measure trace levels of bisphenol A (BPA) in infant urine, a simply improved high-performance liquid chromatography (HPLC)-tandem mass-spectrometry method was developed and validated. In the present work, a ZORBAX SB-C18 column (2.1 × 30 mm, 3.5 μm) was used for trapping and isolating BPA from the HPLC mobile phase. The result showed that the trapping column can provide effective separation from the background BPA to the sample BPA. This method has a limit of detection (LOD) of 0.048 μg/L, spiked recoveries 85.4% (0.50 -10.0 μg/L), and relative standard deviations of 1.8 -15.9%. By using its low-LOD advantage, we firstly reported that Chinese infants (n = 48 with age <6 months) have been ubiquitously exposed to BPA (detection frequency of 93%) with an median level of 0.13 μg/L (ranging from below LOD to 5.04 μg/L).

Detection of bisphenol A and derivatives in human urineviacyclodextrin-promoted fluorescence modulation

“…Reported methods for the detection of BPA and BPA derivatives focus predominantly on the use of mass spectrometry based methods, including gas chromatography-mass spectrometry (GC-MS) 24 and high-pressure liquid chromatography (HPLC) 25 with mass spectrometry detection. 26 While these methods provide exquisite sensitivity for even trace amounts of BPA, their use comes with significant drawbacks in terms of the cost associated with such methods, the level of expertise required to operate this instrumentation, and the time required to achieve high levels of purification that enable such sensitive detection.…”

A Very Simple Method for Detection of Bisphenol A in Environmental Water by Heme Signal Amplification

“…Consequently, accurate quantitative analysis for BPA is critical for protecting human health, the environment and certify safety. [7][8][9][10] In order to detect BPA with sensitivity, numerous analytical techniques have been developed, typical techniques including mass spectrometry accompanying with gas chromatography (GC-MS), 11 mass spectrometry accompanying with liquid chromatography (LC-MS), [12][13][14] high-performance liquid chromatography (HPLC), 15,16 fluorescence detection, 17 immunoassay, 18,19 and electrochemical methods. [20][21][22][23] The simple operation, low-cost, portability, sensitive response and rapid detection make electrochemical techniques ideal for analytical methods.…”