INFECÇÃO POR Mycoplasma pulmonis EM RATOS WISTAR PROVENIENTES DE BIOTÉRIO

Infecção por micoplasma e efeitos inflamatórios em roedores criados em laboratório para pesquisa experimental Infección por micoplasma y efectos inflamatorios en roedores criados en laboratorio para investigación experimental ABSTRACT Mycoplasma pulmonis is the agent of murine respiratory mycoplasmosis and is the most frequently isolated species from laboratory rats. One of the important consequences of its infection is the reduction of breeding rates and significant interferences in the experiments using these animals. Its experimental infection is also considered a model for studying human infection by M. pneumoniae. We have recently reported M. pulmonis infection rates of up to 75% in rodents bred in facilities for experimental research; however, its effect in the animals' immune system was not evaluated. Therefore, the aim of this work was to investigate the effect of M. pulmonis in rats bred at the animal facility of University of Blumenau, by polymerase chain reaction and culture, in order to verify the viability of the use of these animals for experimentation. Inflammatory parameters, such as C-reactive protein (CRP) and alpha-1 acid glycoprotein (A1GP), as well as haematological parameters were determined from blood samples. It was observed a positivity rate of 80% of M. pulmonis infection. It was also noticed that mycoplasma infection was associated with increased CRP and A1GP levels, as well as blood monocytes. Considering that the possibility of mycoplasma infection is a well-known issue, and that the routine examinations and preventive measures have been well standardised internationally for many years, the findings of this study indicate that the use of the evaluated animals is inacceptable for experimental research. It is strongly advisable that laboratories which conduct this kind of research should be assured of the absence of mycoplasma infection in the animals were used.

show abstract

Mycoplasma infection and inflammatory effects on laboratory rats bred for experimental research

Ceola

Sampaio

Blatt

et al. 2016

Rev Pan-Amaz Saude

View full text Add to dashboard Cite

show abstract

Parasitological, epidemiological, and hematological findings in Spix's yellow-toothed cavy (Galea spixii Wangler, 1831) in the State of Paraiba, northeastern Brazil

Gomes

Vieira²,

Coradi³

et al. 2022

Semina: Ciênc. Agrár.

View full text Add to dashboard Cite

Brazil is composed of large ecosystems with vast biodiversity in fauna and flora. Agriculture and livestock farming provides an interaction between humans and domestic and wild animals, which favors the dissemination of infectious and parasitic agents. Wild rodents present a wide ecological diversity; therefore, it is necessary to know which species may be carriers of pathogens. This study aims to identify endo and ectoparasites, and hemoparasites in Spix's yellow-toothed cavy (Galea spixii Wangler, 1831), and determine the hematological parameters of this species. The study was carried out with 16 adult specimens from both sexes, captured in the rural areas of Remigio and Areia, Paraiba State, northeastern Brazil. All Spix's yellow-toothed cavies presented ectoparasites, Gliricola quadrisetosa, Gyropus ovalis, Laelaps sp. and Chirodiscoides caviae, and endoparasites identified by the presence of Nematode eggs, Cestode eggs, and coccidian oocysts in the stool samples. To the best of our knowledge, this is the first report of parasitism by Gyropus ovalis, Laelaps sp. and Chirodiscoides cavia in Spix's yellow-toothed cavy. Animals tested negative by PCR for hemotropic Mycoplasma sp., Babesia sp., Ehrlichia sp., and Bartonella sp. For the first time, the hematological profile of these animals was determined. These results can be the basis for future research with the species, which can be used as a sample animal in experiments in clinical settings as an epidemiological guide since this species is routinely used by impoverished populations in the Northeastern region of Brazil as pets, as well as a source of protein.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

INFECÇÃO POR Mycoplasma pulmonis EM RATOS WISTAR PROVENIENTES DE BIOTÉRIO

Cited by 2 publications

References 16 publications

Mycoplasma infection and inflammatory effects on laboratory rats bred for experimental research

Mycoplasma infection and inflammatory effects on laboratory rats bred for experimental research

Parasitological, epidemiological, and hematological findings in Spix's yellow-toothed cavy (Galea spixii Wangler, 1831) in the State of Paraiba, northeastern Brazil

Contact Info

Product

Resources

About