Infected malnourished children displayed changes in early activation and lymphocyte subpopulations

Nájera-Medina, Oralia; Valencia-Chavarría, Fernando; Cortés-Bejar, Consuelo del Carmen; Palacios-Martínez, Monika; Rodríguez-López, Carmen; González-Torres, María Cristina

doi:10.1111/apa.13930

Cited by 7 publications

(4 citation statements)

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“…Studies of undernourished children in sub-Saharan Africa have found lower NK cell numbers than in adequately-nourished children, despite similar NK cell proportions [summarized by Rytter et al (45)]. In a small number of undernourished Mexican children with infections, NK cell numbers were also lower than adequately-nourished children with or without infections; numbers were not restored by nutritional rehabilitation (107). Defects in NK cell numbers in a Nigerian cohort coincided with low serum interferon levels and hyporesponsiveness to interferon stimulation in vitro (108); both defects normalized after nutritional recovery (109).…”

Section: Introductionmentioning

confidence: 99%

Current Understanding of Innate Immune Cell Dysfunction in Childhood Undernutrition

2019

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Undernutrition affects millions of children in low- and middle-income countries (LMIC) and underlies almost half of all deaths among children under 5 years old. The growth deficits that characterize childhood undernutrition (stunting and wasting) result from simultaneous underlying defects in multiple physiological processes, and current treatment regimens do not completely normalize these pathways. Most deaths among undernourished children are due to infections, indicating that their anti-pathogen immune responses are impaired. Defects in the body's first-line-of-defense against pathogens, the innate immune system, is a plausible yet understudied pathway that could contribute to this increased infection risk. In this review, we discuss the evidence for innate immune cell dysfunction from cohort studies of childhood undernutrition in LMIC, highlighting knowledge gaps in almost all innate immune cell types. We supplement these gaps with insights from relevant experimental models and make recommendations for how human and animal studies could be improved. A better understanding of innate immune function could inform future tractable immune-targeted interventions for childhood undernutrition to reduce mortality and improve long-term health, growth and development.

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Section: Introductionmentioning

confidence: 99%

Current Understanding of Innate Immune Cell Dysfunction in Childhood Undernutrition

2019

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“…Then, 3 mL of lysis solution was added and incubated for 10 min. After washing, cells were fixed with 1% p-formaldehyde and stored under conditions of darkness until analyzed through flow cytometry (within the first 24 h) [32,33].…”

Section: Leukocyte Measurementmentioning

confidence: 99%

DASH Diet as a Proposal for Improvement in Cellular Immunity and Its Association with Metabolic Parameters in Persons with Overweight and Obesity

et al. 2021

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The development of obesity entails a chronic low-grade inflammatory state with increased pro-inflammatory cells, mainly in visceral adipose tissue (VAT). Additionally, dietary patterns have an influence on the regulation of chronic inflammation. Dietary Approaches to Stop Hypertension (DASH) include foods with an anti-inflammatory profile and that have positive impacts on body composition (BC), suggesting improvements in inflammatory processes. Objective: To analyze the impact of the DASH diet on cellular immunity, anthropometric, biochemical and BC parameters in patients with overweight and obesity, who could present metabolic syndrome. Methodology: Lymphocyte subpopulations, biochemical parameters, anthropometric parameters, and BC before and 8 weeks after intervention with the DASH diet in persons with overweight and obesity were measured. Results: Fifty-nine young adults participated in the study. After the intervention, no significant changes in biochemical parameters were observed, although a significant decrease in nearly all of the anthropometric and BC variables was found: waist circumference (p < 0.001), percentage and kilograms of fat (p < 0.001 and p < 0.025, respectively), VAT (p < 0.020), and weight (p < 0.001), as well as total lymphocytes and double-positive TCD4+ cells. A relation between changes in leukocyte subpopulations (monocytes, natural killer, helper and cytotoxic lymphocytes, and naive TCD4+ cells) and metabolic improvements (glucose, triglycerides, total cholesterol and LDL-c) was also found. Conclusions: The DASH diet promotes positive changes in lymphocyte subpopulations, anthropometric parameters and BC in persons with overweight and obesity. Future studies should elucidate the cellular and molecular mechanisms through which the DASH diet produces inmunometabolic improvement.

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“…Posteriormente a la incubación, la solución de lisis fue añadida a cada tubo para la eliminación de eritrocitos, y las células fueron lavadas con PBS. Las células fueron fijadas usando paraformaldehido al 1%(Nájera-Medina et al, 2017).ISSN online 2007-9621 Palacios Martínez, M., Nájera Medina, O., González López, M., Ramírez Navarro, K., Solís Chávez, P. C., & Gutiérrez Cárdenas, E. M. Evaluación de subpoblaciones linfocitarias en individuos seropositivos a Helicobacter pylori | 1-11 Análisis por citometría de flujo Los datos fueron obtenidos y analizados usando el software FACSDiva y el citómetro de flujo modelo FACSCanto II (Becton Dickinson Imumunocytometry Systems; San Jose, CA), calibrado con perlas CaliBRATE. En cada evento se analizaron 10 000 células.…”

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Evaluación de subpoblaciones linfocitarias en individuos seropositivos a Helicobacter pylori

Martínez

Medina²,

López³

et al. 2022

Acta Universitaria

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Las infecciones por Helicobacter pylori (H. pylori) son consideradas un problema de salud pública debido a su altoíndice de morbilidad en la población mexicana. Por ello, este trabajo está enfocado a la detección de anti- IgA-H.pylori por medio de la prueba de ELISA y la evaluación de subpoblaciones linfocitarias por citometría de flujo en sangreperiférica de población adulta de una zona rural del estado de Morelos. Se trabajó con 79 participantes adultos (edadde 42 ± 13.7 años), de los cuales 57% (n = 45) fueron seropositivos y 43% (n = 24) seronegativos. No se encontrarondiferencias estadísticamente significativas en leucocitos, ni en células efectoras, solo una pequeña tendencia a aumentar las células de memoria CD4+ y CD8+ en individuos seropositivos a H. pylori, así como una disminución en células CD8+ dobles positivos (CD8+CD45RA+CD45RO+, p < 0.05). Probablemente no existen diferencias relevantes debido a la capacidad de la bacteria para evadir la respuesta inmunológica.

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Infected malnourished children displayed changes in early activation and lymphocyte subpopulations

Abstract: After therapy, malnourished infected children showed poor NK cell responses during the infection's resolution phase, suggesting a persistent malnutrition-mediated immunological deficiency.

Cited by 7 publications

References 24 publications

Current Understanding of Innate Immune Cell Dysfunction in Childhood Undernutrition

Current Understanding of Innate Immune Cell Dysfunction in Childhood Undernutrition

DASH Diet as a Proposal for Improvement in Cellular Immunity and Its Association with Metabolic Parameters in Persons with Overweight and Obesity

Evaluación de subpoblaciones linfocitarias en individuos seropositivos a Helicobacter pylori

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